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Citations for Roche :
1451 - 1500 of 1666 citations for Recombinant Mouse Cd79a protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Cancer Biology 2024Quote: Total proteins were extracted from cultured NB cells using RIPA buffer supplemented with protease and phosphatase inhibitor cocktail (Roche). The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2020Quote: ... Signals were detected using anti-mouse secondary antibodies conjugated with alkaline phosphatase using BCIP/NBT substrates (Roche Biochemical, Mannheim). Similarly ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... roots were incubated overnight at 4°C in anti-myc-mouse primary antibody (1:250, Roche in blocking solution), washed 3 times in PBST ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred into a new tube and was mixed with mouse monoclonal anti-GFP antibodies (11814460001, Roche) that had been pre-conjugated with Protein-G Dynabeads with dimethyl pimelimidate using a protocol described in (Unnikrishnan et al. ...
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bioRxiv - Cell Biology 2021Quote: ... transferred to PVDF membranes (Amersham Hybond) and probed with following antibodies: mouse anti-HA-HRP (1:1000) (12013819001, Roche) rabbit anti-EMC6 (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked with 3% skim milk in TBS for 30 minutes and then probed with mouse anti-GFP (1:1,000, Roche) or rabbit anti-aldolase (Mesén-Ramírez et al. ...
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bioRxiv - Cell Biology 2022Quote: ... with SDS-PAGE and immunoblots performed by standard methods using the following mouse monoclonal antibodies: anti-GFP (11814460001, Roche) and anti-MYC (clone 4A6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfer membranes were then incubated with a 1:10,000 dilution of primary mouse anti-6xHis antibody (Roche, cat. #11922416001) for 1 h ...
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bioRxiv - Pathology 2020Quote: Adult mouse pancreas from Sox9: eGFP transgenic mice (33) were harvested and digested in 1.4 mg/mL collagenase-P (Roche) at 37 °C for 20-30 min ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for in donkey anti-mouse FITC antibody diluted in 10 X blocking buffer (1:100; Roche) + 0.3 % Triton-X100 for 1 h ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... the nonspecific staining was blocked with 10% normal donkey serum in PBS with 1% BSA and 0.3% triton for 1 hour and then incubated with mouse anti-BrdU (1: 1000, Roche), rabbit anti-Ki67 (1 ...
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bioRxiv - Molecular Biology 2022Quote: Both primary neuron and mouse tissues were lysed in Radioimmunoprecipitation Assay (RIPA) buffer and a cOmplete protease inhibitor (Roche). Tissues were lysed using a hand lyser ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG, RRID:AB_262044 or monoclonal mouse anti-GFP, 1:1,000, Roche #11814460001, RRID:AB_390913) at 4°C ...
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Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... primary antibodies used to detect GRASP signals was mouse monoclonal anti-GFP (1:200, Cat# 11814460001, Roche Applied Science). Samples were then washed in PBS-T at 4 ℃ for 10 min for 3 times ...
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bioRxiv - Neuroscience 2023Quote: ... then transferred for 48 hrs in a solution of mouse anti-synaptophysin (1:20, Roche Applied Science, Indianapolis, IN). Sections were then washed in PBS-t 3x10 min ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Microbiology 2020Quote: ... The predicted structure was send to the FunFOLD2 server for the prediction of protein–ligand interactions (Roche et al., 2013). In addition ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Cell Biology 2020Quote: ... post-nuclear lysates pre-cleared using CL-4B Sepharose beads followed by immunoprecipitation with anti-HA-antibody (12CA5) and Protein G agarose (Roche). Beadbound radiolabelled substrates were resuspended in 2 x Laemmli buffer (+20 mM DTT) ...
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Cell Biology 2020Quote: ... generation of total protein from MEF cells was contained by homogenization of cells in lysis buffer containing protease inhibitor (Complete, Roche). For determination of G/F-actin ratio fractionation was performed as described in Huang et al ...
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bioRxiv - Genomics 2021Quote: Reversal of crosslinks and protein degradation: The chromatin samples were incubated with 200 μl proteinase K digestion buffer containing 30 μg Proteinase K (Roche), 0.2 M NaCl and 30 mM Tris-HCl at 65°C for 12 hours.
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified for 30min at 15,000xg and the supernatant was immunoprecipitated for 2h at 4°C with Dynabeads-protein G covalently coupled to the rat anti-HA antibody (Roche). The beads were washed 3×5 min ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were extracted from cells using ice-cold RIPA buffer with complete protease inhibitor mixture (Roche Applied Science, 04693124001). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Lysis buffer was supplemented with protease and protein phosphatase inhibitor tablets (04-693-159-001 and 04-906-845-001 respectively, Roche). Protein content was determined with the BCA Protein Assay kit (23225 ...
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bioRxiv - Developmental Biology 2022Quote: ... Collected tissues were ground in ice-cold protein lysis buffer (Beyotime, Shanghai, China) containing protease inhibitors cocktail (Roche, Basel, Switzerland) for protein extraction ...
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bioRxiv - Biochemistry 2019Quote: ... 50 µl of the supernatant was kept as input and the remaining supernatant was used for immunoprecipitation with the indicated antibody coupled to protein A-Agarose (Roche) or in case of FLAG with anti-FLAG M2 Affinity gel (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: Total proteins were extracted in RIPA buffer containing 1 mM sodium orthovanadate and Complete Protease Inhibitor Cocktail (Roche Applied Science) and quantified using the Pierce™ BCA protein assay kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Cell Biology 2019Quote: For MS analyses immunoprecipitates on Protein-G Dybabeads were washed 2x with TEAB Buffer (100mM) and incubated with Trypsin (Roche) at 37°C for 18 hours ...
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified by centrifugation and incubated with α-Pk clone SV5-Pk1 (AbSerotec) orα-HA clone 12CA5 (Roche) antibodies for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Microbiology 2020Quote: Further protein extraction steps for each of 10 samples were carried out in solutions supplemented with cOmplete protease inhibitors (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Neuroscience 2022Quote: ... At DIV 17 cells were washed with ice-cold PBS and immediately extracted with ice-cold T-PER protein extraction buffer (Pierce) supplemented with inhibitors for proteases and phosphatases (Roche). The lysates were centrifuged at 15000 g for 15 minutes at 4°C and the supernatants were used for further analysis.
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bioRxiv - Neuroscience 2022Quote: ... Lysates were diluted to a final total soluble protein concentration of 1 mg/ml in TBS containing cOmplete mini protease inhibitor tablet (Roche). For AD ...
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were isolated from cell cultures in mid-exponential phase in Hepes 50mM supplemented with EDTA-free protein inhibitor cocktail (Roche). Cell walls were broken by three 30-s cycles at 10000 rpm in the presence of micro glass beads (200-400 µm diameter Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...