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Citations for Roche :
1451 - 1500 of 5982 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... sections were blocked with 10% HI-serum for 2 h and incubated overnight at 4°C with alkaline phosphatase-labeled sheep anti-dig antibody (11093274910, Roche; 1:2500). After washing ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues were minced and then digested at 37°C for 20 min with an enzyme cocktail (collagenase A, dispase and DNaseI, Roche Applied Science). Single-cell suspensions were prepared by filtering through a 70-μm strainer and passing through a nylon mesh ...
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bioRxiv - Immunology 2021Quote: Adipose and other tissues were removed from isolated thymi which were subsequently subjected to three rounds of enzymatic digestion for which cut up lobes were incubation at 37°C in Liberase digestion buffer (PBS Gibco 1X, Liberase Roche 2.5 mg/ml, DNase Roche 10 mg/ml). Cells were incubated with anti-CD45 beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... cut into small pieces and digested for another 1 h at 37°C in 1 mg/ml Collagenase D (Roche, Basel, Switzerland). To obtain single-cell suspensions ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated alone or with ipICP4/pICP4 or BSA for 1 h at 4°C and subsequently fluorescence intensity was measured in a LightCycler II (Roche, Milan, Italy) by observing 6-carboxyfluorescein (6-FAM ...
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bioRxiv - Biophysics 2019Quote: ... or for confocal in a alkaline-washed glass-bottomed chamber coated with human plasma fibronectin (25 µg/mL at 4°C overnight in 1/3 X MBS; Roche Molecular Biochemicals) in DFA supplemented with antibiotic/antimycotic (Sigma) ...
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bioRxiv - Physiology 2021Quote: Accurate measuring of plasma urea and creatinine was performed by the central laboratory of the University Hospital of Cologne using a Cobas C 702 (Roche Diagnostics, Germany). Calculation of sample size was performed by P ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Plant Biology 2022Quote: ... and digested into peptides using one round of overnight incubation at 37°C with 1:50 (enzyme:protein) trypsin (Roche, Cat. No. 03708969001) and a second round of incubation for 4 hours at 37°C with trypsin and Lys-C (Wako Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.). After ligation ...
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bioRxiv - Microbiology 2023Quote: ... LAMP amplification reactions were heated at 65° C for an hour and a half in either a LightCycler 96 (Roche, NC, U.S.A.) or a heat block ...
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bioRxiv - Genomics 2023Quote: ... 3 million crosslinked cells were resuspended in 1 mL of ice-cold Hi-C lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.2% NP-40, and 1× Roche protease inhibitors Cat#11697498001) and rotated at 4 °C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: Cell lysates for intracellular chemokine quantification were obtained via repeated freeze-thaw cycles at −80°C of cells suspended in media containing protease inhibitor cocktail (Roche Applied Science) and final centrifugation at 8000g to pellet debris ...
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bioRxiv - Immunology 2023Quote: Pelleted crypts were kept at -80°C and lysed with RIPA buffer supplemented with fresh cOmplete™ protease inhibitor cocktail (Roche 11836170001) and PhosSTOP™ phosphatase inhibitor cocktail (Roche 4906837001) ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... under agitation and then digested at 37°C for 30 minutes with Liberase (0.15mg/ml Liberase TL-Roche 246749-in HBSS/PBS). For both skin and intestine ...
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bioRxiv - Neuroscience 2023Quote: ... in Tris-EDTA buffer pH 7.8 at 95°C for 64 min in standard cell condition solution one (CC1) using a Ventana Discovery ULTRA (Roche Indianapolis IN). Blocking was then performed in an inhibitor solution for 12 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and the striatal slices were incubated at 30°C in Krebs-HCO3-buffer (Mg2+ free) with 10 μg/mL adenosine deaminase (Roche, Basal, Switzerland) for 30 min under constant oxygenation with 95% O2/5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Cell Biology 2024Quote: ... just after the resection the tissue was dissociated into small pieces (∼2 mm × 2 mm × 2 mm) and then digested for 30 minutes at 37°C with Collagenase (2 mg/mL, Roche, Basel, Switzerland) and DNase I (400 U/mL ...
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bioRxiv - Microbiology 2024Quote: ... The engorged individuals were maintained for two days at 25°C and then stored in tissue lysis buffer (Roche, Prague, Czech Republic) at - 20°C in pools of 5 females for subsequent DNA isolation and nested PCR.
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bioRxiv - Molecular Biology 2020Quote: ... targeting the HBV_circ_1 B site was labelled with DIG-11-dUTP by digoxin using a Dig high primer DNA labelling kit and detection starter kit II (Roche). Hepatic tissue paraffin sections of HCC patients were treated with dewaxing and then digested with protease K ...
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bioRxiv - Neuroscience 2021Quote: ... quantified using High Sensitivity DNA Kit run on Agilent 2100 Bioanalyzer and also KAPA Illumina library quantification kit (Roche, 7960140001) run on LightCycler 480 ...
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bioRxiv - Developmental Biology 2021Quote: Final RNA-seq libraries were quantified using the KAPA Library Quantification Kits for Illumina sequencing platforms (Roche, Kit code KK4824). 100bp paired-end sequencing reads (Illumina HiSeq2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from each sample using RNeasy Mini Kit (QUIAGEN) and cDNA was synthesized using Transcriptor First Strand cDNA Synthesis Kit (Roche). qPCR was performed in SYBR Green I Master Mix with sequence specific primers for genes of interest (Table 1. ...
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bioRxiv - Neuroscience 2021Quote: ... was used to capture lncRNAs in accordance with the SeqCap EZ Hybridization and Wash Kit and SeqCap EZ Accessory Kit (Roche). Captured lncRNA libraries were sequenced on an Illumina HiSeq 4000 platform with a read length of 150 bp x 2.
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA was amplified using ResolveDNA Whole Genome Amplification Kit (BioSkryb Genomics) and then libraries were prepared for sequencing (Kapa Hyperplus Kit, Roche). All libraries were sequenced on either an Illumina MiniSeq or NextSeq instrument (Illumina ...
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bioRxiv - Pathology 2022Quote: Total RNA was purified from cells using a Tiangen Easy Fast RNA Extraction kit and reverse-transcribed using Transcriptor First Strand cDNA Synthesis kit (Roche). Expression of mtDNA encoded genes were determined by quantitative real-time RT-PCR using SYBR Green PCR Master Mix (Roche Applied Science) ...
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bioRxiv - Neuroscience 2020Quote: Tissue samples were homogenized and RNA was extracted using an RNAeasy Mini Kit (Quiagen, Hilden, Germany) and reverse transcribed using a Transcriptor First-Strand cDNA Synthesis kit (Roche Diagnostics ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified by gel electrophoresis and extracted from the gel via a commercial kit (HighPure PCR Product Purification Kit, Roche, DE). Sample preparation (Illumina TruSeq amplicon library ...
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bioRxiv - Plant Biology 2019Quote: ... The blots were hybridized with probes that were labeled using PCR DIG Probe Synthesis Kit and hybridization was detected with DIG Luminescent Detection Kit following the manufacturer’s instructions (Roche, Switzerland).
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bioRxiv - Genomics 2021Quote: ... Both libraries were quality controlled using a Fragment analyzer NGS kit (AATI) and qPCR with the Kapa library quantification kit (Roche) prior to sequencing.
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bioRxiv - Genomics 2022Quote: ... whole transcriptome mRNA-enriched RNA-Seq libraries were constructed from cell lysates using a KAPA mRNA Capture Kit and mRNA HyperPrep Kit (Roche) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ug of total RNA was used for the construction of ribosome- depleted sequencing libraries using the KAPA RNA HyperPrep Kit with RiboErase kit (HMR, Roche) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2023Quote: ... anti-DIG antibody (Anti-Digoxigenin-AP Fab fragments) and Chemiluminescent detection with CSPD following the instructions of the kit (all products and kits from ROCHE).
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA with the MagAttract HMW DNA extraction kit and prepared short-inserted paired-end libraries for the whole genome with the KAPA HyperPrep kit (Roche) PCR-free protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting library was assessed with Fragment Analyzer (Advanced Analytical Technologies) using the Genomic DNA Kit DNF-467 and quantified with KAPA Library Quantification Kit (Roche). A single lane of HiSeq3000 was used for sequencing with 2×151 bp read length in presence of 1% PhiX (Illumina) ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... the amount of LDH released during the in vitro experiments were quantified using the kit Cytotoxicity Detection Kit (LDH) provided by Roche. Triton X-100 (Applichem ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAB staining was detected using the UltraView Universal DAB Detection Kit or the OptiView DAB IHC Detection Kit (all from Roche). The following antibodies were used ...
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bioRxiv - Zoology 2021Quote: ... quantified by qPCR Kapa Library quantification kit (Roche) and sequenced on an Illumina MiSeq (PE300 ...
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bioRxiv - Cell Biology 2020Quote: ... Signal was detected using LumiLight Plus Kit (Roche) on the Chemidoc MP imaging system (Biorad).
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bioRxiv - Genetics 2021Quote: ... and a High Pure Plasmid Isolation Kit (Roche), respectively.
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bioRxiv - Microbiology 2021Quote: ... quantified using a Kapa qPCR Library Kit (Roche), and sequenced using a MiSeq 500 v2 nano run (MiSeq Reagent Kit v2 ...
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bioRxiv - Microbiology 2019Quote: ... The Light Cycler 480 probe master kit (Roche) was used to amplify RSV cDNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the KAPA Library Quantification Kit (Kapa Biosystems). Data from sperm cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... quantified using the KAPA library quantification kit (Roche) and sequenced on an Illumina HiSeq 4000 with 2 × 150 bp paired-end runs.
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bioRxiv - Immunology 2020Quote: ... KAPA Hyper Prep Kit for Illumina (KAPA Biosystems), and NEXTflex DNA barcodes (Bioo Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... using KAPA SYBR FAST qPCR KIT (Kapa Biosystems). Fold change expression was calculated using the ΔΔct method ...
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bioRxiv - Molecular Biology 2020Quote: ... and KAPA Library Quantification Kit (Kapa Biosystems, 07960140001). All samples were sequenced on one Illumina HiSeq 2500 lane.