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Citations for Roche :
1451 - 1500 of 6590 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 150 mM KAc, 1 mM MgCl2, 1 mM EGTA, 1 mM DTT, 0.1 mM ATP, 20 mM PMSF, and 10 Roche protease inhibitor tablets per L) and lysed by a dounce homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... 300 mM NaCl, 7.5 mM MgCl2, 0.25 mM EDTA, 1 mM DTT, 1 M Urea, 1 % NP-40, 1x Roche complete, 20 U/mL RNase Out), pipetting up and down 30 times ...
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bioRxiv - Neuroscience 2021Quote: ... and alkaline phosphatase-conjugated anti-DIG antibody (diluted 1:1,000 in DIG-1, Roche Diagnostics) for 2 h and washed twice with DIG-1 for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr at RT and incubated with anti-DIG-POD (1:500, 11207733910, Roche) and rabbit anti-RFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... Then WST-1 assay was performed by adding WST-1 reagent (cell proliferation reagent, Roche) directly to the culture wells ...
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bioRxiv - Neuroscience 2019Quote: ... 1% NP-40 and 1 mM dithiothreitol supplemented with protease (Roche, Welwyn Garden City, UK) and phosphatase (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were subjected to blocking solution (1% Bovine Serum Albumin (Roche, 9048-49-1), 5% Normal Goat Serum (Thermoscientific ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% SDS) including 1 mM PMSF and 1×protease inhibitors (cOmpleteTM protease inhibitor cocktail, Roche) and stored at -20°C in 1 Laemmli buffer until usage ...
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bioRxiv - Cell Biology 2022Quote: EV preparations were lysed using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche) and EV concentration was determined using Pierce™ MicroBCA protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... After blocking for 1-2h with the 1% blocking reagent (Roche Applied Science, cat# 10057177103), sections were incubated with alkaline phosphatase-conjugated anti-DIG antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were immunolabeled with anti-bromodeoxyuridine (BrdU) (Roche 11170376001; 1:250 to 1:500 dilution) to visualize BrU signal.
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bioRxiv - Immunology 2020Quote: ... at 1:1,000 dilution in 1% BSA in PBS and One-step ABTS substrate (Roche). Non-PfCSP reactive antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM NA3VO4 and 1 µg/ml leupeptin supplemented with complete mini protease inhibitor (Roche). Denatured proteins (20-40 µg ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM MgSO4) supplemented with 1 mg/ml lysozyme and cOmplete Protease Inhibitor Cocktail (Roche), and subsequently lysed using sonication ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated overnight with 1:1000 anti-digoxigenin-alkaline phosphate (11093274910 Roche, 1:1000) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM MgSO4) supplemented with 1 mg/ml lysozyme and cOmplete Protease Inhibitor Cocktail (Roche). Cells were lysed by sonication ...
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bioRxiv - Genomics 2021Quote: ... pronase (0.4 mg mL-1) and DNase I (0.1 mg mL-1, Roche, Cat#10104159001), and then incubated for digestion at 37 °C in a water bath for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% Triton and Complete® EDTA-free protease inhibitor cocktail (1 tablet/50 ml; Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated in a 1:1000 dilution of 1 mg/ml DAPI (Roche Diagnostics) in PBS for 5 min followed by three washes with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4) with 1 mM TCEP and 1 tablet complete EDTA-free protease inhibitor (Roche). The lysate was cleared by centrifugation at 30,000 g for 30 min at 4 °C and subsequently filtered through a 0.45 μm filter before incubation with 2.5 ml glutathione-conjugated sepharose beads (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 1 tablet/50 ml cOmplete proteinase inhibitor and 1 mM Pefabloc SC (Roche)) supplemented with 1 % formaldehyde ...
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bioRxiv - Molecular Biology 2023Quote: ... with protease inhibitor (1 mM phenylmethanesulfonyl fluoride and 1 x Complete Protease Inhibitor Cocktail; Roche), then sonicated with a Bioruptor (Diagenode) ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... 1 mM EGTA and 1 mM EDTA) supplemented with cOmplete™ protease inhibitors (Roche, #4693116001) and PhosSTOP™ phosphatase inhibitors (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA) supplemented with 1 mM DTT and protease and phosphatase inhibitors (Roche). Protein concentrations were measured with the Bradford assay (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM MgCl2 and 1%NP-40) supplemented with a protease inhibitor cocktail (Roche, 1:10000). Cell lysates were centrifuged at 10,000xg for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT) containing protease inhibitors (1 mM PMSF, 1x Roche complete protease inhibitor mix). The cells were lysed on ice for 10 min ...
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bioRxiv - Genetics 2023Quote: ... 1% Triton X-100 and 1× cOmplete™ ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor (Roche). After removal of cell debris by centrifugation (twice 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... Western Blotting was performed on 14 day old seedlings to determine protein expression using anti-HA high affinity antibody (Roche, Penzberg, Germany). Primer sequences for RxL21 and RxL21 deletion variants are in Table S9 ...
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bioRxiv - Cancer Biology 2020Quote: ... protein lysates were prepared from HCC1428 cells with control or E2-E10 siRNA knockdown using modified Tissue Protein Extraction Reagent (TPER) (Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The protein lysates were then diluted into 0.5 mg/ml of total protein in SDS sample buffer and denatured on the same day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2021Quote: ... the pellet washed in 1x PBS then re-suspended in 1x protein loading buffer (4x NuPAGE® LDS sample buffer [Invitrogen], 1x PBS and 25 uL ß-mercaptoethanol, 2x Roche cOmplete Mini protease inhibitor cocktail tablets ...
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bioRxiv - Neuroscience 2020Quote: Cells from mouse brain or spleen tissues were lysed with T-PER Tissue Protein Extraction Reagent (Thermo) containing Complete Protease Inhibitor Cocktail (Roche Applied Science). Protein concentrations were determined with BCA protein assay reagents (Pierce) ...
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bioRxiv - Immunology 2022Quote: Anti-SARS-CoV-2 (N protein) IgM/IgG levels in the serum were measured using an Elecsys Anti-SARS-CoV-2 with cobas8000 (Roche Diagnostics KK) at the Department of Clinical Laboratory ...
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bioRxiv - Microbiology 2022Quote: ... The gene encoding this protein was amplified from genomic DNA of the SECphi4 phage using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers AS330+AS331 (Table S6) ...
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bioRxiv - Cancer Biology 2022Quote: Total protein cell extracts were obtained by lysing the cells with RIPA buffer supplemented with 1x protease inhibitors (PI) (Roche, Milan, Italy). Proteins were quantified using the BCA method (Pierce ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Physiology 2023Quote: ... Protein extracts were prepared using T-PER (Pierce Chemical Co., IL) in the presence of protease inhibitors (Roche Applied Science, Barcelona, Spain), and subjected to Western blot analyses with anti-rat anti-ABCA1 antiserum (kindly donated by Dr ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates of 20 μg protein were separated in a SDS-12% polyacrylamide gel (PAGE) followed by transferring proteins to a polyvinylidene difluoride (PVDF) membrane (Roche, catalog 3110040). After blocking in 5% milk (in 1xphosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2022Quote: Mouse liver tissues and cells were homogenized and lysed in Protein Extraction Reagent (P0013, Beyotime Biotechnology) supplemented with protease inhibitor cocktail (04693159001, Roche, Basel, Switzerland). Equal amounts of protein lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... The supernatant was discarded and the pellet was resuspended in a 1:1 solution of 100 mM Tris-HCl pH 7.4 with EDTA-free protease inhibitor cocktail (Roche, 1 tablet in 10ml of buffer) and 4x NuPage LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were lysed in RIPA buffer (50 mM Tris-HCl, 150 mM NaCl, 1% Nonidet P-40, 01% SDS, 1 mM EDTA, supplemented with protease inhibitor cocktail [Complete Roche Mini, 1 tablet per 50 ml]) and spun at 20.000 g to eliminate cell debris ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT) supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche) and lysed by sonication ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT and 1 mM EDTA) containing EDTA-free protease inhibitor cocktail (mini-complete, Roche), 20 μM MG132 and 25 μg/ml ALLN followed by boiling in SDS sample buffer ...