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Citations for Roche :
1451 - 1500 of 8559 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... one microgram of the extracted RNA was converted into complementary DNA (cDNA) using Reverse Transcriptase (Roche-4897030001, Basel, Switzerland), a high-quality enzyme known for its reliable performance ...
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bioRxiv - Cancer Biology 2023Quote: ... One hundred nanograms of total RNA was used for library preparation with the KAPA mRNA Hyperprep Kit (KK8581) (Roche). Each resulting in a uniquely dual-indexed library was quantified and quality accessed by Qubit and TapeStation ...
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bioRxiv - Developmental Biology 2019Quote: ... BMP4 and 6 were prepared by in vitro transcription (DIG RNA labeling kit; Roche Diagnostics). Plasmids to generate cRNA probe for Smad4 was gifted by Prof ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Glucose was measured in the supernatant using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche), for calculation of starch content in glucose equivalents ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were introduced into HEK293T cells using the FuGENE 6 transfection reagent (Roche Applied Science). Cells were selected with puromycin (1 μg/ml) ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed in 12 μL containing 6 μL Kapa HRM-Fast Master mix (Roche), supplemented with 1.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection of HEK-293 cells was performed with FuGene 6 transfection reagent (Roche, Mannheim, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and the released glucose was assayed using the hexokinase/glucose-6-phosphate dehydrogenase assay (Roche). For mature grains ...
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bioRxiv - Neuroscience 2023Quote: qPCR was performed on a QuantStudio 6 Flex using the SYBR Green Master mix (Roche). In all cases ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were resuspended in approximately five volumes of lysis buffer (50 mM NaH2PO4 pH 8, 1 M NaCl, 10 mM Imidazole, 2 mM PMSF and protease inhibitor cocktail (Roche #4693132001). Cells were lysed by sonication on ice for 12 x 30 seconds at 30 W with one minute on ice in between ...
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bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
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bioRxiv - Plant Biology 2021Quote: ... 90 mM KCl, 2 mM EDTA, 0.5 mM spermidine, 0.2 mM spermine, 0.5 mM EGTA, 1× Roche Complete protease inhibitor cocktail). Nuclei were isolated by sucrose density gradient centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... mouse Hepa 1-6 and rat Fao hepatoma cells at 75% confluency were incubated with a mixture of oleate and palmitate (ratio 2:1, total concentration 1.2 mM) coupled to FA-free Bovine Serum Albumin (BSA) (Roche Applied Sciences). All fatty acid stocks were initially reconstituted in absolute ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours at room temperature with an anti-FL antibody conjugated with HRP (1/500, Roche Diagnostics) followed by reaction with TSA biotin reagents (Perkin Elmer ...
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bioRxiv - Neuroscience 2019Quote: ... 0.5% Triton X-100, 100 mM NaF, 1 mM ortho-vanadate, 2 mM EDTA, and a protease inhibitor cocktail [Roche 11836153001]). Lysates were vortexed and cleared by centrifugation (15,000 x g for 15 min at 4°C) ...
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bioRxiv - Molecular Biology 2019Quote: ... primary or re-amplified WTA products) added to 1 μl Expand Long Template Buffer 2 (Expand Long Template PCR System, Roche Diagnostics), 1 μl dNTPs (10 mM each) ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 150 mM NaCl, 1% NP-40, 2 mM EGTA, and complete protease and phosphatase inhibitors from Roche, Basel, Switzerland). Cell lysate was cleared by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... 1 M KCl, 2 mM EDTA, 1 mM DTT, 0.01 U/ml benzonase and 1x EDTA-containing protease inhibitor cocktail [Roche Applied Sciences]). The lysate was cleared by centrifugation at 18,000 x g for 45 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... plus phosphatase and protease inhibitors (2 mM NaVO4, 10 mM NaF, and 1 mM PMSF) and Complete protease inhibitor cocktail (Roche, CA). For tissue extracts ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteins from mixed samples (14N-flg22/15N-mock and 14N-mock/15N-flg22) were extracted with IP buffer 2 with 1× Protease Inhibitor (Pierce) and phosphatase inhibitor (Roche, PhosSTOP), and centrifuged for 1700g for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were extracted in ice-cold lysis buffer (150 mM NaCl, 2 mM EDTA, 40 mM HEPES, and 1% Triton X-100) supplemented with phosphatase (Roche #04906837001) and protease inhibitor cocktail ...
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bioRxiv - Physiology 2022Quote: ... 2 mM EDTA, 20 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF, 1X cOmplete™ Protease Inhibitor Cocktail, Roche) was added to the fragmented chromatin ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT, 2 μM MG-132 proteasome inhibitor (#S2619, SelleckChem) and cOmplete® ULTRA EDTA-free inhibitor cocktail (#5892953001, Roche). Approximately 0.1 ml of 0.6 mm glass beads were added and thoroughly mixed ...
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bioRxiv - Neuroscience 2023Quote: ... followed incubation with blocking solution for 2 hours before incubation with alkaline phosphatase (AP)-conjugated anti-DIG Fab fragments antibody (1:1000, Roche, #11093274910) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The tissue was weighed and digested with 10μL mg−1 DISC lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% TritonX-100, 10% glycerol, H2O, protease inhibitor cocktail tablet: Roche, Basel, Switzerland). The tissue was lysed with TissueLyser85300 (Qiagen ...
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bioRxiv - Systems Biology 2021Quote: The digested lysates were mixed 1:1 with 2x IP buffer (200 mM Tris, pH 8.0; 600 mM NaCl; 4% Triton X-100; 2x Roche Complete EDTA-free protease inhibitors), and then kept on ice for no more than a few hours prior to antibody addition ...
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bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes were hybridized at 56°C overnight and subsequently detected by anti-DIG at 4°C overnight (1:1000; Roche 11-207-733-910) with secondary tyramide signal amplification for 2h at room temperature in the dark (AKOYA Biosciences ...
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bioRxiv - Zoology 2024Quote: ... Samples were pre-incubated for 2 h in blocking solution (25% deactivated goat serum in PBT) and incubated overnight at 4°C in blocking solution with anti-DIG antibody conjugated to alkaline phosphatase (Roche Diagnostics, Germany; 1:2000). After several washes in PBT ...
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bioRxiv - Cell Biology 2022Quote: ... pellets were resuspended in 100 µL of lysis buffer (50 mM Tris pH 7.5, 1 mM EDTA, 3 mM DTT, 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche], 1.1 mM PMSF, and 1X Pepstatin A) and beaten on a bead-beater for 5 minutes at room temperature with 100 µL of acid-washed glass beads (cat ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed with 4% SDS lysis buffer (4% SDS, 150 mM NaCl, 50 mM triethanolamine pH 7.4, Roche protease inhibitor, benzonase). Protein concentrations were determined by the BCA assay (Pierce) ...
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bioRxiv - Developmental Biology 2021Quote: ... The sample was incubated for 2 h with 2 μl of 20 mg/ml ProteinasK (Roche), and subjected to AMPure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were blocked in 2% TNB (2% blocking reagent (Roche, REF 11 096 176 001) in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50 ...
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bioRxiv - Immunology 2023Quote: ... the medium was supplemented with 30 IU/mL recombinant human interleukin 2 (IL-2) (Proleukin, Roche). Electroporated T cells were kept in culture ON and then used directly or frozen.
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bioRxiv - Microbiology 2024Quote: ... interleukin-2 (IL-2; specific activity 10 U/ng) at concentration of 20 ng/mL (Roche) and phytohemagglutinin (PHA ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... 4 cOmplete EDTA-free Protease Inhibitor Cocktail tablets (Roche), 500 U benzonase ...
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bioRxiv - Cancer Biology 2019Quote: ... for 4 minutes and Bluing reagent (Roche #760-2037) for 4 minutes.
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bioRxiv - Cancer Biology 2021Quote: ... 4 mL of Red Blood Cell Lysis Buffer (Roche) were added before being gently rocked for 10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... (4) we used KAPA HiFi master mix (Roche, 07958935001) and 19 cycles of PCR.
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bioRxiv - Cancer Biology 2024Quote: ... SDS 0.4%) + Proteinase-K 4 mg/ml (Roche, 3115887001) and incubated for 2h at 55°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μL of 20x PhosStop® phosphatase inhibitors (Roche), 4 μL of 20x cOmplete® protease inhibitors (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μL of 20x cOmplete® protease inhibitors (Roche) and 1.6 μL of 1 M DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... dry milk or 2% BSA (Roche), membranes were probed with primary antibody (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA fraction V (Roche Diagnostics), 50 μM 2-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... 2 mg/mL Collagenase VIII (Roche) and 0.5 mg/mL DNAse I (Roche ...