Labshake search
Citations for Roche :
101 - 150 of 2737 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... End point cell proliferation was assayed with ELISA 5-bromo-2′ -deoxyuridine (BrdU) kit from Roche Diagnostics (Sigma– Aldrich) ...
-
bioRxiv - Immunology 2022Quote: ... was added and cells were centrifuged at 350g for 7 min at 4°C and resuspended in PBS with 0.5% BSA (Roche) and 1% FBS (Sorting Buffer) ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated for 15 minutes with 4 μL of X-treme Gene HP DNA transfection reagent (Roche). Transfected cells were selected for by hygromycin resistance ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein lysate of 10-20 μg was run on 10–15% SDS-PAGE gel and transferred toa PVDF membrane (Roche, USA). The membrane was incubated for 60 min at room temperature in 5% BSA solution ...
-
bioRxiv - Systems Biology 2022Quote: ... 15-μl reactions (10 ng cDNA) were performed on a LightCycler® 96 System (Roche). The change in the gene expression was calculated using 2(-ΔΔCt ...
-
bioRxiv - Microbiology 2023Quote: ... 15 μg RNA in 50 μl water was digested with 10 U DNAse I (Roche). The quality of the RNA was controlled by determination of the RNA Integrity Index using Agilent BioAnalyzer High Sensitivity DNA Assays ...
-
bioRxiv - Molecular Biology 2020Quote: Proliferative capacity of cells was analysed using 5-bromo-2’-deoxy-uridine (BrdU) labelling (Roche, Basel, Switzerland). Cells seeded on coverslips were pulsed with 10 μM BrdU for 60 min at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... dahliae Ls.17 (using the KAPA HiFi DNA polymerase, Roche) for complementing hex1 deletion strains ...
-
bioRxiv - Molecular Biology 2022Quote: ... blots were hybridized with 10 pmol/ml DIG-labeled (CAG)7 (5′-gcAgCagcAgca-3′) at 70°C for 4 h in hybridization buffer (5 × SSC, 1% block solution [Roche, Switzerland] ...
-
bioRxiv - Physiology 2024Quote: ... from two bees were pooled and homogenized in 250 µL of phosphate-buffered saline (7 mM NaCl, 2.7 mM KCl, and 10 mM PO4, pH 7.4) containing cOmplete protease inhibitors (Roche) at kept on ice until further processing ...
-
bioRxiv - Cancer Biology 2023Quote: Proliferation was measured by using 5-bromo-2’-deoxy-uridine (BrdU) labelling and detection kit (Roche, BSL, CH) as described earlier (25) ...
-
bioRxiv - Cell Biology 2022Quote: Sense and anti-sense DIG-labelled RNA probes were synthesised from 5’ and 3’ regions of tert using DIG RNA Labelling Mix (Roche). A 562bp 3’ region of tert ...
-
bioRxiv - Genetics 2024Quote: ... Nuclei were extracted by resuspending in 100 μL nuclei extraction buffer per 1-2.5 M cells (20 mM HEPES pH 7.5, 10 mM NaCl, 0.5 mM spermidine, 0.1% BSA, 0.1% NP-40, and 1 tablet Roche mini protease inhibitor per 10-15 mL) and incubating for 10 minutes on ice ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Tissues were washed in three changes of 0.2× SSC at 44°C for 15 minutes each and blocked in 4×SSC containing 1% blocking reagent (Roche) in 4× SSC for 1 hour at RT ...
-
bioRxiv - Plant Biology 2024Quote: ... the lysate was clarified by centrifugation at 12,000 x g and 4°C for 15 min and then filtered using a 0.45-µm syringe filter (Roche). The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were washed in MABTr and then incubated in NBT (nitro blue tetrazolium)/BCIP (bromo-chloro-indolyl-phosphate, Roche) for colour development ...
-
bioRxiv - Neuroscience 2023Quote: ... cells and sections were processed for BrdU immunodetection following the manufacturer’s recommendations (5-Bromo-2′-deoxy- uridine Labelling and Detection Kit I, Roche). The slides were mounted for fluorescent microscopy in ProLong® Diamond Antifade Mountant with DAPI (Life Technologies).
-
bioRxiv - Physiology 2024Quote: Fibroblast proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) incorporation assay (colorimetric) from Roche (Indianapolis, IN, USA) for 48 h following the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
-
bioRxiv - Immunology 2024Quote: ... Epidermal sheets were digested in Liberase™ (13 U/ml, Roche), for 1.5h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Transfected DRG neurons were fixed 15 min in 4 % paraformaldehyde (PFA) and hybridized with DIG-labeled GFP cRNA probes (Roche) as described previously (Merianda et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 μg/ml insulin (Roche, #11376497001), and 15 mg/ml transferrin (Roche ...
-
bioRxiv - Plant Biology 2024Quote: ... 15 mM MgCl2, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, 10 mM β-mercaptoethanol, 2 mM PMSF and Roche cOmplete Protease Inhibitor Cocktail)) were added in the ground leaves and incubated for 30 min at 4 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate; Roche) until the staining appeared (overnight or up to 48 h) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3×10−4 M MTG and 300 μg/ml human transferrin (Roche, 10652202001)) in a triple vent petri 10 cm dishes (Thermo fisher ...
-
bioRxiv - Cell Biology 2022Quote: ... MEFs were incubated for 4 h with 10 ng/ml colcemid (Roche, 10295892001). Cells were then collected and incubated for 15 min at 37 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were extracted using extraction buffer (50 mM Tris-HCl pH 7.5, 20 mM NaCl, 10% glycerol, 1% Triton, and a 1:7 ratio of protease inhibitor; 04693124001, Roche, Basel, Switzerland), based on a previously described extraction buffer (55) ...
-
bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
-
bioRxiv - Cancer Biology 2020Quote: Cell (1 × 104) proliferation was measured using the 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit III (Roche, Mannheim, Germany) (Jin ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche, 03010040001). The membranes were probed with the indicated primary antibodies followed by the appropriate HRP-conjugated secondary antibodies ...
-
bioRxiv - Immunology 2022Quote: ... tissues were minced and placed into gentleMACS C tubes (Miltenyi 130-093-237) containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001] ...
-
bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
-
bioRxiv - Developmental Biology 2024Quote: ... cut into small pieces and placed into 15 mL tubes containing 10 mL sterile DMEM (without serum) supplemented with 0.5 mg/mL Liberase DL (Roche). Digestion was performed at 37°C for 90 minutes while shaking ...
-
bioRxiv - Cancer Biology 2022Quote: ... 7 μl proteinase inhibitor 7X (Roche, 04693159001) and 5 μl phosphatase inhibitor 10X (Roche ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.025% pronase (Roche, 7 U/mg)] in complete DMEM/F-12 medium in a spinner flask ...
-
bioRxiv - Cancer Biology 2021Quote: ... and TP53 (DO-7) (platform Ventana, Roche).
-
bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 10 % (v/v) glycerol) supplemented with: cOmplete protease inhibitor (Roche), 1 mM PMSF ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total RNA was reverse-transcribed using 2.5×10-4 U/μl hexanucleotide mix (Roche), 0.4mM deoxynucleotide mix (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2020Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
-
bioRxiv - Molecular Biology 2022Quote: ... washed in PBS and resuspended in 0.5 mL lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg·mL-1 lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany) and incubated at 37°C for 20 min ...
-
bioRxiv - Neuroscience 2020Quote: ... SE was stopped after 1H by two injections of Diazepam (Valium) with a 15 min interval (10 mg/kg, i.p. Roche, France). Pilocarpine-treated animals were then observed periodically for general behavior and occurrence of spontaneous seizures ...
-
bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... Any contaminating DNA in the samples was removed by treating 15 µg of RNA with 10 U of DNase I (Roche) for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio) ...
-
bioRxiv - Cancer Biology 2023Quote: ... ssDNA deamination activities were measured using WCE prepared using 100 μL HED lysis buffer per 1 million cells (25 mM HEPES, 15 mM EDTA, 10% Glycerol, 1 mM DTT, and 1 protease inhibitor tablet [Roche]). Samples were sonicated in a water bath sonicator to ensure complete lysis ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
-
bioRxiv - Cell Biology 2024Quote: ... and the tissue was oxygenated in Leibovitz’s L-15 medium containing 10 mM BDM and 0.75 mg/ml of Collagenase/Dispase® mixture (Roche, 10269638001), then placed in a shaking incubator for 30 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... 15 µg/ml transferrin (Roche), 450 µM monothioglycerol (Sigma) ...
-
bioRxiv - Genomics 2022Quote: ... 15 U/μL TdT (Roche), 0.1 U/μL RNase H (QIAGEN ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... CaCo-2 and Vero cells seeded at a density of 5 × 103 cells and 7 x 103 per well in 200 μl media containing 10% FBS in a 16-well E-plate (Roche Applied Science, Germany), respectively ...