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Citations for Roche :
101 - 150 of 254 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates (100–200 μg) were then immunoprecipitated with GFP antibody (Roche Applied Science). Ubiquitination was detected as described previously (38).
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were prepared using the IP lysis buffer (0.5% NP40 (Roche, Basel, Switzerland), 1x cOmplete protease inhibitor (Roche ...
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bioRxiv - Genetics 2020Quote: ... fly head or cell lysates were lysed in RIPA buffer with protease inhibitors (Roche) and passed through a 27-gauge needle to shear DNA ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared by lysing in RIPA buffer with protease inhibitor cocktail (Roche) and protein concentrations were normalized using a BCA assay (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were diluted with 500uL of RIPA buffer with protease inhibitor cocktail (Roche) and subjected to immunoprecipitation with G1 anti-DYKDDDDK affinity resin overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Cell lysates were prepared in RIPA buffer supplemented with complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth ...
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bioRxiv - Biophysics 2023Quote: ... The lysate supernatant was mixed with 200 µL of Ni-nitrilotriacetic acid resin (Roche Complete His-Tag purification resin ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysates were then supplemented with a 1× solution of protease inhibitor (Roche Diagnostics) and 1× solution of PhosSTOP.
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Bioengineering 2023Quote: Cell lysate or conditioned medium were harvested and supplemented with protease inhibitor cocktail (Roche). After centrifugation at 14,000xg for 30 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: We then targeted the N-(nucleoprotein) gene of SARS-CoV-2 virus with Roche Lightcycler 480 RNA Master Hydrolysis Probes (Roche Basel, Switzerland) with primers developed in house ...
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bioRxiv - Microbiology 2020Quote: We obtained OP and NP samples from one hospitalised COVID-19 patient who had tested virus-positive three weeks earlier (cobas® SARS-CoV-2 test, Roche diagnostics), from three individuals who had previously tested SARS-CoV-2 positive ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
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bioRxiv - Pathology 2022Quote: RNA was reverse-transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (LifeTechnologies, Carlsbad, CA) on the LightCycler 480 or LC96 instrument (Roche, Indianapolis, IN), and quantified by interpolation onto a standard curve made up of serial tenfold dilutions of in vitro transcribed RNA ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were made in RIPA-buffer supplemented with Protease inhibitor cocktail (11836170001, Roche), 1mM NaF and 1mM NaVO3 ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with 30 μl pre-washed rat anti-HA Affinity Matrix (Roche) for 3 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... protein lysates were prepared in RIPA buffer supplemented with complete protease inhibitor (Roche Applied Science). Proteins were then separated on 6%–15% SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: Lysate destined for Ribo-seq was incubated with 1500 units of micrococcal nuclease (Roche 10107921001) and 6 uL of SUPERase-In at 25 C for 1 hour and shaken at 1400 rpm ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates were prepared in RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Lysates were subjected to SDS PAGE electrophoresis and transferred onto PDVF membrane (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: Whole-cell lysates were obtained using RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). An amount of 10 µg of protein was then resolved on 10-12.5% polyacrylamide gels and transferred to nitrocellulose membranes using the semi-dry technique ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates sampled with phospho-specific antibodies were also lysed with phosphatase inhibitors (phosSTOP™, Roche). Lysates were then centrifuged at 20,000 g ...
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bioRxiv - Biochemistry 2019Quote: ... Clear lysate was affinity-purified by incubating it with cOmplete His-Tag purification resin (Roche) for 2 hours (4°C) ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The cell lysate was pre-cleaned with protein G agarose beads (Roche Diagnostic, Basel, Switzerland) for 1 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2022Quote: ... Remaining lysate was rotated O/N with 25 µl of Protein-A-agarose beads (Roche) and 2 µg of mouse anti-HA antibody (Supplementary Table S1) ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 cell lysates were prepared in RIPA buffer containing protease inhibitors (Roche Diagnostics, Indianapolis, IN). To examine mitochondrial-associated proteins ...
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bioRxiv - Developmental Biology 2024Quote: ... The remaining lysate was rotated overnight with 50 µl of Protein A-agarose beads (Roche) and 2 µg of mouse anti-HA (monoclonal ...
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Total DNA was isolated from L3 larvae or 5-days old adults of Iduna homozygous mutants and control sequencing strain using the Roche genomic DNA extraction kit (Roche). To confirm the mutant line ...
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bioRxiv - Microbiology 2019Quote: ... AHSV4 WT strain (isolated in Morocco in 1990 (80)) and EEV3 WT strain (isolated in South Africa in 1974 (81)) were amplified by RT-PCR (Roche) from purified infected-cell RNAs ...
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bioRxiv - Microbiology 2022Quote: ... The ietAS system was amplified from genomic DNA of the donor strain using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601) with primers OSM646 and OSM647 (Table S6) ...
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bioRxiv - Genomics 2023Quote: ... All strains were sequenced using an Illumina MiSeq platform and the KAPA LTP library preparation kit (KAPA Biosystems, Wilmington, MA). The pooled libraries were loaded into a MiSeq system and sequenced using a MiSeq reagent kit v2 with 2 × 250 cycles (Illumina ...
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bioRxiv - Immunology 2021Quote: Splenocytes lysates were prepared using Cell Lysis Buffer (#9803, CellSignaling Technology) supplemented with protease inhibitors (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Genetics 2019Quote: ... Lysates were diluted in RIPA buffer with 1 µM PMSF and Complete Protease Inhibitor Cocktail (Roche) to a concentration of 1.68 mg/ml and 500 µl was used for capture of biotinylated proteins on streptavidin beads and subsequent mass spectrometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysates (1 μg/μL) were enzymatically digested with 50 μg/mL pronase E (Roche) at 37°C for 1 hour followed quenching with protease inhibitors and SDS-PAGE loading buffer ...
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bioRxiv - Molecular Biology 2019Quote: Total protein lysates were prepared using RIPA lysis buffer containing protease inhibitors (Complete Ultra Tablets, Roche). Equal amounts of total lysates were separated by SDS-PAGE (4–15% ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell lysate was harvested with RIPA buffer containing protease inhibitors (protease inhibitor cocktail tablets, Roche) on the third day ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Cell Biology 2020Quote: ... whole cell lysate of primary myoblasts was prepared by lysing buffer containing protease inhibitor cocktail (Roche). Protein concentration was determined with the BCA Protein Assay Reagent (Pierce Biotechnology ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3 h at 4 °C ...
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bioRxiv - Pathology 2022Quote: Tissue and cell lysates were produced in RIPA buffer supplemented with PhosSTOP (Roche Diagnostics, Indianapolis, IN) and protease inhibitor cocktail (Roche Diagnostics) ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...