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Citations for Roche :
101 - 150 of 910 citations for Yellow Fever virus Envelope protein sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or p89.6) and an envelope deficient HIV-1 backbone vector (pSG3Δenv) using XtremeGENE HP DNA Transfection Reagent (Roche). After 24 hours of incubation at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... pCW57.1_yeastAOX 32 with the Delta-Vpr packaging plasmids and the VSV-G envelope plasmid (DNA concentration ratio, 1.2: 1: 0.3) into HEK293T cells using X-tremeGENE 9 Transfection Reagent (Roche). Lentivirus-containing media was harvested at 24 hr after fresh media change ...
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bioRxiv - Neuroscience 2021Quote: ... 20% heat-inactivated sheep serum) and incubated with anti-DIG antibody (1:2’000, Roche) overnight ...
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bioRxiv - Neuroscience 2020Quote: ... DIG-labeled nucleotides were detected using Anti-Digoxigenin-Fluorescein (sheep polyclonal, Roche, 1:75) incubated overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and secondly with anti-DIG3 (sheep anti-digoxigenin fluorescein conjugate, 1:200, Roche Diagnostics) and streptavidin-Cy3 conjugate (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... embryos were incubated with primary antibodies at 1:375 dilution (sheep anti-DIG, Roche cat #11333089001 and mouse anti-Biotin ...
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bioRxiv - Molecular Biology 2019Quote: ... labeled with alkaline phosphatase–conjugated sheep anti-DIG FAB fragments (11093274910, Roche, Basel, Switzerland), before visualized with Liquid Permanent Red (DAKO ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with alkaline phosphatase-conjugated sheep anti-digoxigenin antibody (1:2000; Roche) and the secondary antibodies overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by incubation with sheep anti-DIG antibody conjugated to alkaline phosphatase (Roche, Switzerland) (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... The slides were incubated in blocking solution (10% sheep serum, 2% blocking reagent (Roche), 0.3% Tween-20 in MAB ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... Sections were incubated with sheep anti-FITC-POD (1:2000, Roche cat# 18 11426346910) or sheep anti DIG-POD (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was generated by co-transfecting HEK293T cells with the lentiviral construct pCMV-dR8.91 (Δ8.9) plasmid and the pMDG envelope-expressing plasmid using X-tremeGENE 9 DNA Transfection Reagent (Roche, 6365779001). Viral supernatant was collected within 24-48 h post-transfection for subsequent infection of the target cells.
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bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
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bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
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bioRxiv - Microbiology 2020Quote: ... and incubated overnight with rat anti-HA tag (Roche) and mouse anti-Actin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... and incubated with cOmplete His-Tag purification resin (Roche) for 16 hours on a tube roller (Starlab ...
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bioRxiv - Molecular Biology 2022Quote: ... HA tag (Roche 11666606001, 1:1,000 or 1:3,000), His tag (Abcam ab18184 ...
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bioRxiv - Plant Biology 2020Quote: ... 12 µl of complete His-Tag Purification Resin (Roche) was added and incubated for 15 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... or Complete His-Tag Purification Resin (Roche, Basel, Switzerland), that had been prewashed with respective lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... then incubated with His6-tag Purification Resin (Roche, 05893682001) at 4 °C for 1 hour ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... the tissues were immediately incubated with sheep anti-digoxigenin-AP (alkaline phosphatase; Roche Diagnostics, Germany) at a dilution of 1:1000 in PBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were incubated with sheep anti-digoxigenin-alkaline phosphatase (AP) antibody (1:3000, Roche) for 90 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with the alkaline phosphatase-conjugated sheep anti-DIG (1:500; 11093274910, Roche Diagnostics) and biotinylated rabbit anti-DNP (1:100 ...
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bioRxiv - Systems Biology 2019Quote: ... along with the packing plasmid psPAX2 (16.2 μg) and envelope plasmid pMD2.G (1.8 μg) were transfected using Fugene 6 (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Cells were then transfected with the Tet-pLKO-puro plasmids targeting either UHRF1 or DNMT1 transcripts and the accompanying lentiviral packaging (psPAX2) and envelope (pMD.2G) plasmids with Opti-MEM and Xtreme HP Gene transfection reagent (Roche) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
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bioRxiv - Microbiology 2022Quote: ... using the LightCycler Multiplex DNA Virus Master kit (Roche Diagnostics, Mannheim, Germany) on a LightCycler 480 instrument (Roche Diagnostics).
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bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... including 5 μL template using LightCycler Multiplex RNA Virus Master (Roche, Switzerland). All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Cell Biology 2019Quote: ... 3) 2hr RT incubation in blocking solution (5% Sheep Serum, 1% Roche Blocking Buffer in PBST); 4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digoxigenin FISH signals were amplified using sheep anti-digoxigenin fluorescein fab fragments (1:50, Roche 11207741910) and fluorescein rabbit anti-sheep antibodies (1:100 ...
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bioRxiv - Biophysics 2022Quote: ... The AFS chamber was next functionalized with polyclonal anti-digoxigenin from sheep (Sigma, Roche cat. 11333089001) by injection of 50 μL of a 200 μg/mL anti-Dig solution (20 min incubation) ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were incubated with horseradish peroxidase (HRP) conjugated sheep anti-digoxigenin antibody (1:100, Roche) overnight at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin-labelled probes were then detected by incubating with sheep Anti-Digoxigenin-POD Fab fragments (Roche) at a 1:50 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... FITC-labelled probes were detected first by incubating with sheep Anti-Fluorescein-POD Fab fragments (Roche) at a 1:50 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... an anti-HA-tag antibody (#11867431001) was purchased from Roche, an anti-Flag-tag antibody (#012-22384 ...
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bioRxiv - Cell Biology 2019Quote: ... rat anti-HA-tag mAb (clone 3F10, Roche, no. 11867423001), rabbit anti-SEC61A mAb (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Clarified supernatants were rocked with Complete His-Tag resin (Roche), 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Ulp1 and cleaved affinity tag were removed by NiNTA (Roche). The NiNTA flow through was adjusted to 250mM NaCl and purified on HiTRAP-SP (20mM HEPES pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 4mL cOmplete His-Tag Purification Resin (Roche) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml of the cOmplete His-Tag purification resin (Roche) equilibrated with the Tris buffer 4 containing 25 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using cOmplete His-Tag Purification Resin (Roche, Switzerland) and His-Bind Purification kit (Novagen ...
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bioRxiv - Developmental Biology 2021Quote: ... Signals of transcripts were detected using Anti-DIG-AP (alkaline phosphatase) and Fab fragments from sheep (Roche) and developed in Fast Red TR and naphthol-AS-MX-phosphate in 0.1 M Tris-HCl pH 8.2 (tablet set ...
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bioRxiv - Molecular Biology 2021Quote: ... which was labelled with digoxygenin (DIG) and detected with sheep anti-DIG FITC (11207741910, Roche, RRID: AB_514498) and rabbit anti-sheep FITC antibodies (FI-6000 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... For NBT/BCIP staining sections were incubated overnight with sheep anti-DIG-AP (1:1000, Roche 11093274910). After washing staining was performed using NBT/BCIP in NTMT until satisfactory intensity ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation with HRP-conjugated rabbit anti-sheep antibodies and detection using ECL reagents (Roche Diagnostic GmbH). A series of timed exposures were undertaken to ensure that densitometric analyses were performed at exposures within the linear range ...
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bioRxiv - Physiology 2020Quote: ... The sections were incubated with sheep anti-DIG antibody (1:200, Roche Applied Science; cat. no 1333 089), biotinylated donkey anti-sheep antibody (1:200 ...