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Citations for Roche :
101 - 150 of 10000+ citations for Thyroxine T4 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... BrdU incorporation was carried out according to the manufacturer′s specifications (Cell Proliferation ELISA, Roche) with an incubation time of 5 hours as previously described.23 Quantification was performed by measuring the absorbance using Power Wave 340 ELISA reader from Bio-Tek.
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bioRxiv - Synthetic Biology 2019Quote: ... Restriction enzymes were purchased from New England Biolabs (Ipswich, MA, USA) and T4 ligase (Roche, Basilea, Switzerland) was used for ligation purposes ...
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bioRxiv - Cell Biology 2020Quote: ... 3 min) and plated onto 1.5 % agar TAP plate containing 10 µg mL-1 hygromycin B (Roche) and 20 µg mL-1 of paromomycin sulfate (Fisher BioReagents).
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bioRxiv - Genomics 2020Quote: ... Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche) in a total volume of 16µl ...
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bioRxiv - Cancer Biology 2020Quote: ... CAL33-c5 and CAL33-c11were seeded in 96-well plates and cell viability was determined with the colorimetric Cell Proliferation Kit II (XTT) (Roche) 24 ...
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bioRxiv - Molecular Biology 2022Quote: ... The clones were washed off the plates and the total plasmid was extracted using High-pure plasmid isolation kit (Roche).
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bioRxiv - Microbiology 2023Quote: ... five microliter of elution was loaded into a 96-well plate with the Light Cycler RNA master hybridization Probe kit (Roche) and amplification was performed in a Lightcycler 480 machine (Roche ...
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bioRxiv - Physiology 2020Quote: ... in 384-well plates (Roche) and each reaction was run in duplicate ...
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bioRxiv - Microbiology 2024Quote: ... in 384-well plates (Roche). The total reaction volume per well was 3 µl ...
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bioRxiv - Immunology 2022Quote: Full-length HIV clones were quantified by reverse transcriptase (RT) enzyme-linked immunosorbent assay (ELISA) (Roche) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 900 µl of ligation mix was added (120 µl 10x ligase buffer, 50 units of T4 ligase (Roche) and 770 µl MilliQ ...
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bioRxiv - Cancer Biology 2022Quote: ... Embryos were transferred to 24 well plates and NTMT-NCP-BCIP mix (1:200 dilution NCP-BCIP:NTMT (Roche)) was added ...
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bioRxiv - Biochemistry 2023Quote: ... LDH kit and WST-1 reagent were purchased from Roche. Bleomycin sulphate was purchased from Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed in 384-well plates (LightCycler 480 Multiwell Plates 384, white, Roche Diagnostics) on the Light Cycler 480 II instrument (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: Adherent macrophages in 6-well plates were washed with PBS containing 1 mM sodium orthovanadate and 10 mM 1,10-phenanthroline (Roche) on ice prior to lysis ...
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bioRxiv - Immunology 2021Quote: ... and 384-Well Reaction Plates (Roche). Primer sequences are as follows:
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bioRxiv - Microbiology 2023Quote: ... using Lightcycler 480 multiwell plates (Roche) and Luna Universial qPCR Master Mix (NEB ...
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bioRxiv - Genomics 2019Quote: ... 1μg of this material was then ligated in a volume of 20 μL with 1μl of T4 DNA ligase (Roche, 10799009001) at 16°C to generate a randomized library of large fragments ...
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bioRxiv - Bioengineering 2020Quote: ... The corresponding fragments were inserted in miRVec expression plasmid (restricted with the same enzymes) by ligation with T4 ligase (Roche) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The mixture was re-incubated 20 min at 75°C and the purified DNA product further incubated overnight at 16°C with a T4 DNA ligase (Roche).
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bioRxiv - Molecular Biology 2021Quote: ... pre-let-7 and pre-miR-16 molecules (miRVana) were radioactively 5’-labeled with 32P isotopes by incubation with 32P-ƴATP and the phosphorylating enzyme T4 PNK (Roche) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche), and transformed into XL10-Gold E ...
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bioRxiv - Genomics 2022Quote: Plasmatic free T3 and free T4 were quantified on a Cobas 6000 automat with the Cobas e601module (Roche, ECL analysers).
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bioRxiv - Microbiology 2023Quote: ... a 2.3 kb-homologous DNA fragment was cloned into a unique SpeI site of a non-replicative plasmid (pIJ10700) using T4 DNA ligase (Roche). The fragment was amplified using Q5® DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... as recommended by the manufacturer (Cell Proliferation Kit 1, 11465007001 Roche). After overnight incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: ... LN were pierced and torn with sharp forceps in 24-well plates and incubated for 15 min at 37° C in 1 ml digestion buffer (100 U/ml collagenase A (Roche), 500 U/ml collagenase D (Roche) ...
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bioRxiv - Immunology 2019Quote: ... memory B cells were plated at 6 B cells in 55 μl per well into 96 × 384-well plates in B cell media supplemented with 100 U ml−1 IL-2 (Roche), 50 ng ml−1 IL-21 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: Cells confluent in a 15-cm dish were washed by PBS buffer three times before being lysed on plate with 1 mL ice-cold NP-40 lysis buffer supplemented with protease inhibitor cocktail (Roche) and PhosSTOP (Roche) ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Immunology 2023Quote: Cell viability upon transduction of tBID death reporter was assessed in 96-well plates through WST-1 assay (Roche, Switzerland) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were seeded to confluency on the microelectrodes of the E-plate (E-plate 16, Roche Applied Science). Electrical impedance readings were taken every 2 min for 5h ...
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bioRxiv - Genomics 2019Quote: ... and AdRb (5′ -TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16°C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65°C for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Purified and digested DNA was ligated to PCR products at 16°C overnight in a final volume of 20 μl using 2 U of T4 DNA ligase and 1x ligation buffer (Roche, 10481220001). Plasmid DNA was transformed into TOP10 chemically competent cells (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and AdRb (5′-TCCTCGGCCG-3′) were ligated to the DpnI digested fragments in an overnight reaction at 16° C using T4 DNA ligase (Roche, 799009). After incubation the ligase was heat-inactivated at 65° C for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... The incorporation of BrdU was then measured by ELISA at 24h per the manufacturer’s protocol (Roche Diagnostics, Mannheim, Germany).
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bioRxiv - Cell Biology 2021Quote: Transduced MEFs were seeded in triplicate in a 96 well flat bottom tissue culture plate and WST-1 assay (Roche, Switzerland) performed as per manufacturer’s protocol at day 0 ...
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bioRxiv - Bioengineering 2022Quote: ... Encapsulated or adherent cells were plated in a 96 well plate for 24 or 48h when WST-1 reagent (Roche, Switzerland) was added ...
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bioRxiv - Microbiology 2022Quote: Single cell suspensions from the ear were obtained by using tweezers to separate the dorsal and ventral sides of the mouse ear and placing the sheets dorsal-side down in a 24 well plate with 1 mL/well of RPMI1640 containing 0.25 mg/mL of Liberase TL (Roche, Diagnostics Corp.) and 10 μg/mL of DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μl of the lysis buffer was added to each 6-well plate along with 1×Protease Inhibitor Cocktail (PIC, Roche cOmplete). The cells were incubated in a rocker at 4°C for 1hr ...
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bioRxiv - Developmental Biology 2023Quote: ... HUVEC were seeded at a density of 60,000cells/well of the E-plate (E-plate 16, Roche Applied Science), then electrical impedance readings acquired every 2 min for 24 hr ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was briefly pulsed in the centrifuge and sealed with a clear PCR plate seal (Roche Life Science). The LightCycler® instrument (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using Lightcycler 480 SYBR Green 1 Master Kit (Roche). PCR amplification was performed as follows ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability assay was performed using a WST-1 assay kit (Roche). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and co-transfected into 60-70% confluent BHK cells grown in 6-well plate tissue culture plates using Fugene HD transfection reagent (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 250-800 ng DNA per well of a 12-well plate or 500 ng per well of a 6-well plate using X-tremeGENE 9 DNA transfection reagent (Roche), and harvested after 24 h.
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bioRxiv - Microbiology 2020Quote: ... thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Cell Biology 2019Quote: ... and levels of p24 were determined by ELISA as previously described (65) The SEAP levels of the supernatants were determined by a chemiluminescent method (Roche) and were used to normalize the differences in transfection efficiencies.
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bioRxiv - Immunology 2020Quote: ... To quantify NETs we measured HNE-DNA complexes using ELISA specific for HNE and anti-DNA Ab of Cell Death Detection ELISAPLUS (Roche). Briefly ...