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Citations for Roche :
101 - 150 of 652 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Protein-G agarose beads were manufactured by Roche. All other reagents were from Sigma-Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... The protein was digested with AspN protease (Roche) at 30°C for 16 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ul of Protein A-agarose beads (Roche) was then added to the extract before overnight incubation ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...
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bioRxiv - Neuroscience 2019Quote: Total proteins from mammalian cells were extracted from cells by resuspension and homogenization in RIPA buffer containing protein inhibitors (Complete, Roche Diagnostics) and phosphatase inhibitors (Phospho-STOP ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The probes were isolated through gel filtration columns (mini Quick Spin RNA Columns, Roche) and stored at −20°C.
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bioRxiv - Microbiology 2022Quote: ... Specific DNA fragments were isolated using an agarose gel extraction kit (Roche Applied Science). Restriction enzyme digests were carried out using enzymes purchased from New England Biolabs by following the manufacturer’s recommended procedure ...
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bioRxiv - Molecular Biology 2023Quote: Samples fractionated on polyacrylamide gels were electroblotted to polyvinyl difluoride (PVDF) membranes (Roche, Germany) as described before [16,38] ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μl of slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... were incubated with specific antibodies overnight and then with 60 μL of a slurry of Protein A- or Protein G-agarose beads (Roche, Mannheim, Germany) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were detected using ECL lumi-light plus (Roche) and images acquired using camera system (Chemitouch Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Proteins were transferred to nitrocellulose or PVDF membranes (Roche) in tris-glycine buffer at 110 V for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and protein extracted with ELB buffer containing PhosSTOP (Roche) and 50 μM sodium fluoride ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor and phosphatase inhibitor (Complete-EDTA free, Roche/Sigma prod ...
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bioRxiv - Cancer Biology 2019Quote: BSA Protein Assay Kit (A8020-5, Roche, Basel, Switzerland) was applied to measure the protein concentration after lysing BC cells with RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 1/10 volume of Protein-G agarose beads (Roche) was then added and the sample rotated for 30 min at RT °C ...
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bioRxiv - Immunology 2019Quote: Proteins were digested on beads using trypsin (Roche, Germany) in 6 M Urea ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 80V for 1.5 hours ...
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bioRxiv - Biochemistry 2019Quote: ... and then incubated with protein A–agarose beads (Roche) for 4 h ...
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bioRxiv - Genomics 2019Quote: ... protease inhibitors) and precleared with protein A beads (Roche) at 4 °C for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitations were performed using Protein A agarose beads (Roche). DivJ was immunoprecipitated using an anti-DivJ antiserum (15) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were semi-dry blotted onto PVDF membranes (Roche), which were subsequently blocked in 5 % milk in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with Protein G beads (Roche, 1124323301) for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto polyvinylidene fluoride (PVDF) membrane (Roche) for 30V for 16 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Neuroscience 2021Quote: ... Then homogeneous protein G-agarose suspension was added (Roche) to the suspension ...
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bioRxiv - Pathology 2019Quote: ... Proteins were detected using polyclonal antibodies against GFP (Roche), Histone H3 (GeneTex ...
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bioRxiv - Plant Biology 2021Quote: ... coupled to 50µl bed volume Protein A agarose (Roche) per sample ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM DTT and 1x protein inhibitor cocktail (Roche), and incubated at 25ºC for 1 h on a rotating wheel ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with protein G-agarose beads (Roche) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred onto PVDF membranes (Roche, Basel, Switzerland), and Western blot detection was carried out as previously described [32,33] ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were reduced using 10 mM dithiothreitol (Roche) and alkylated with 50 mM iodoacetamide ...
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bioRxiv - Cancer Biology 2023Quote: ... Following electrophoretic transfer of proteins onto PVDF membranes (Roche), nonspecific binding was blocked by incubation with 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... 400 µg of protein were reduced using DTT (Roche, Cat#10708984001 ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with protein A agarose beads (Roche, #11134515001) at 4 °C overnight with shaking ...
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bioRxiv - Microbiology 2020Quote: ... EMSAs were performed on cell extracts using a DIG Gel Shift Kit (2nd generation) (Roche). DNA fragments were labeled at their 3’ end with digoxigenin-11-ddUTP using terminal transferase ...
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bioRxiv - Plant Biology 2020Quote: ... Gel pieces were dried by vacuum centrifugation and incubated with trypsin (modified sequencing grade, Roche) in 50 mM NH4HCO3 or Glu-C (sequencing grade ...
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bioRxiv - Plant Biology 2020Quote: The RNA gel blot was done by using the DIG Northern Kit (Roche, Mannheim, Germany). The blot was probed with part of the GFP transcript (Supplementary Table 1).
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bioRxiv - Zoology 2020Quote: ... Each gel also contained 32P-end-labeled size ladders (Molecular Weigh Marker XV, by Roche Diagnostics and 1Kb DNA ladder by New England Biolabs) ...
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bioRxiv - Plant Biology 2022Quote: ... The 5’-end biotin probes were generated using a DIG Gel Shift Kit (Roche, China) (Supplemental Table S8) ...
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bioRxiv - Plant Biology 2023Quote: ... EMSA followed the manufacturer’s protocol mentioned in the 2nd generation DIG gel shift kit (Roche).
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bioRxiv - Immunology 2023Quote: ... DNA from 1% agarose gel was capillary transferred to a positively charged nylon membrane (Roche) and cross-linked by UV irradiation by standard procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein were extracted from cells and tissues using protein lysis buffer supplemented with protease and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Protein concentrations were assayed by the Pierce™ BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... For protein extraction cells were resuspended in protein extraction buffer (1xPBS, 10% glycerol, 1mM EDTA, 1mM PMSF, 1x Roche cOmplete™Protease Inhibitor Cocktail), and lysed in a bead beater ...
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bioRxiv - Plant Biology 2019Quote: ... Protein extract was incubated overnight with anti-HA antibodies (Roche). After over-night incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were incubated with 30 µl Protein G-Agarose (Roche) per ml lysate for 1 hour at 4 °C and washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... followed by incubation with Protein G Agarose beads (Roche, 1124323301) (15μl ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl bed volume of Protein A Agarose (Roche 11134515001) was added the following day to each sample ...