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Citations for Roche :
101 - 150 of 181 citations for L Asparagine H2O 13C4; D3; 15N2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Neuroscience 2023Quote: ... Hematocrit (0.5 to 0.64) and blood glucose concentration (5.7 to 8.6 mmol/L, Accu-Chek Performa Nano, Roche, Mannheim, GER) were measured shortly before injecting the radiotracer.
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bioRxiv - Molecular Biology 2023Quote: 3-5 day old males of the indicated genotypes and experimental groups were homogenised in 150µl of homogenisation buffer (0.2%Triton X-100 supplemented with 1 x complete mini EDTA-free protease inhibitor (Roche) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was washed twice with 300 μl sodium phosphate buffer (20 mM Na-phosphate pH 6.8, 1 mM PMSF, 1× Mini Protease Inhibitor Cocktail, Roche) with centrifugation at 16,000g for 20 min between washes ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... the beads were resuspended with 1 ml VIB and subsequently 600 µl of Elution Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1x cOmplete EDTA-free protease inhibitors (Roche), 20 mM β-Glycerophosphate ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Neuroscience 2021Quote: ... and resuspended in 25μL ice cold ATAC-RSB buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP40 (Roche cat#11332473001)) ...
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bioRxiv - Molecular Biology 2020Quote: ... In a 50 μl-reaction the concentrated DNA (15 μl) was mixed with 10 μl of the Nextera index mix (i5 + i7) and 2x Kapa HiFi Master Mix (Roche). The PCR was performed in a Thermocycler (Eppendorf mastercycler ...
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bioRxiv - Physiology 2021Quote: ... and 400 ml of sample was placed in a 2-l wide-mouthed Erlenmeyer culture flask with 100 ml of freshly prepared blendzyme (Roche Liberase TM ...
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bioRxiv - Plant Biology 2021Quote: ... The construct was used to transform wild type plants following the standard floral dip method (Clough and Bent, 1998) and selected with 50 mg L-1 Hygromycin (Roche). A total of 60 T1 plants were individually genotyped by PCR and sequencing ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.78 g/L CSM-His and 20 g/L agar) and screened for expression of Erg6-GFP by Western blot with anti-GFP bodies (11814460001; Roche) and for localization of Erg6-GFP to LDs by fluorescence microscopy.
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bioRxiv - Developmental Biology 2021Quote: ... brn3a1 and brn3a2(l) transcripts were visualized with anti digoxigenin-alkaline phosphatase conjugates and BCIP/NBT or BM purple substrates (Roche). For two color WISH krx-20 (Oxytoby and Jowett ...
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bioRxiv - Genetics 2021Quote: ... The cultures were spun down and cell pellets were resuspended in 500 μL of Workman Extract Buffer (40mM HEPEs pH7.4, 250mM NaCl, 0.1% NP40, 10% Glycerol, 1 mM PMSF, Roche proteinase inhibitors). 250 μL of glass beads were added and cells were lysed with Peqlab precellys homogenizator (3×30 sec) ...
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bioRxiv - Plant Biology 2021Quote: ... Two µL of diluted cDNA was used as a template for the qRT-PCR with Fast SYBR Green Master Mix (Roche) on a Light-Cycler 96 instrument (Roche ...
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bioRxiv - Immunology 2020Quote: ... The mucosa was stripped and dissociated in GentleMACS tubes in digestion medium (DM; complete medium (RPMI 1640 with PGA/L-glutamine/10% FCS) with 1 mg/mL Collagenase D (Roche), 1 mg/mL soybean Trypsin inhibitor (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... water was exchanged for 100 µL water with 5 µM L-012 (WAKO chemicals) and 2 µg/mL horseradish peroxidase (Type II, Roche). After the leaf discs were treated with 1 µM 3-OH-C10:0 (stock in MeOH ...
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bioRxiv - Cancer Biology 2022Quote: 1 μL amplified cDNA library was used as template in a 29-cycle PCR reaction using KAPA HiFi HotStart ReadyMix (Roche). To avoid possible PCR-induced library biases ...
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bioRxiv - Microbiology 2022Quote: ... The solution was incubated for 20 min at 37°C and 15 μl of proteinase K (20 g L-1l; Roche) was added ...
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bioRxiv - Microbiology 2022Quote: ... A cell pellet was resuspended in 250 μL Tris-EDTA buffer and 50 μL 30 g L-1 lysozyme (Roche) in Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
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bioRxiv - Genomics 2024Quote: ... or 3 µl of direct lysis extract were used as template in 50 µl PCR reactions using KAPA HiFi HotStart ReadyMix (Roche), primers P3/4 and P5 (Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... the supernatant was removed and the pellet was resuspended in 250 µL of Galbraith buffer pH 7.0 plus 0.1% Triton X-100 and 1x cOmplete Protease Inhibitor Cocktail (Roche, Mannheim, Germany). 50 µL of 0.2 µm glass beads were added and samples were vortexed for 3 minutes to smash open spores and release nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μL 1 M spermidine, 500 µL 10% Triton X-100, and 10 ml glycerol in 38 ml dH2O, 1 Roche Complete Protease Inhibitor EDTA-Free (Millipore Sigma 11873580001)) ...
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bioRxiv - Immunology 2023Quote: ... 1 vial dissolved in 5mL HBSS to yield 20U of papain/mL in 1mM L-cysteine with 0.5mM EDTA) and 1mg/mL DNAse I (Roche 10104159001) with curved scissors ...
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bioRxiv - Immunology 2023Quote: ... 0.5 –1 L of the crude lysates were treated with 5 mg/mL each of DNase I and RNAse (Roche) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... blocked for 1 h in blocking reagent (100 mM Tris HCL pH 8; 150 mM NaCL; 5 g/L Blocking Reagent (#11096176001, Roche)) and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA ...
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Genomics 2023Quote: ... were put on ice for 15min then resuspended in 250μl chilled douncing buffer (10mM Tris-HCl pH7.5, 4mM MgCl2, 1mM CaCl2) with 1X Protease Inhibitory Cocktail (PIC, Roche 5892791001). Cells were homogenized through a 1ml 25g 5/8 syringe for 20 times and kept on ice ...
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bioRxiv - Biochemistry 2023Quote: ... were used to inoculate each well of a 96-well plate containing 196 μL of LB media (supplemented with ampicillin, and 200uM IPTG) The plate was covered with transparent LightCycler480 Sealing Foil (Roche), and grown for 24 h in a Biotek Synergy H1 plate reader at 42 °C with double orbital shaking ...
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bioRxiv - Genetics 2024Quote: ... The 5μl total volume reaction was loaded in 384-well plates and was performed in a LightCycler 480 Instrument II (Roche), using a cycling program of ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Genomics 2024Quote: ... Adapters were ligated by adding 15 µL of the ligation mix (3 µL T4 Ligase Buffer 10X, 0.5 µL T4 ligase (5 U/µL, Roche, 10799009001), 0.25 µl of x-Gene Stubby Adapter 50 mM (IDT) ...
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bioRxiv - Cell Biology 2024Quote: ... and the tissue was oxygenated in Leibovitz’s L-15 medium containing 10 mM BDM and 0.75 mg/ml of Collagenase/Dispase® mixture (Roche, 10269638001), then placed in a shaking incubator for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Molecular Biology 2021Quote: 200 μL of eluate was extracted on the Magna Pure 96 using a DNA and Viral NA Small Volume Kit (Roche, 06543588001) with the universal small volume protocol and eluted into 50 μL proprietary elution buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Animals were then re-suspended on ice in 50 µl of RIPA buffer with added phosphatase inhibitors (Cell Signalling Technologies #9806) and complete EDTA-free protease inhibitor cocktail (Roche # 04693159001) before sonication ...
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Microbiology 2021Quote: ... 0.2 pmoles of gene-specific forward primer and 0.2 pmoles of gene-specific reverse primer was combined with 2 µL of cDNA template into each well of a 96-well plate (LightCycler® 480 Multiwell Plate 96, white, 04729692001, Roche). qPCR amplification was performed using the following cycling conditions ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2020Quote: ... and 6.8 μL ddH2O were used and the RT-qPCR was performed using a Light Cycler 96 Real-Time PCR Machine (Roche, Germany). Expression levels were calculated using the 2-ΔCT equation (Kilambi et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of cDNA were used as a template in a 5- l reaction mixture from a KAPA SYBR FAST qPCR kit (Kapa Biosystems). Primers used are listed in table S3 ...