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Citations for Roche :
101 - 150 of 1612 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Cytotoxicity was assessed by measuring the release of lactate dehydrogenase (LDH) into the culture supernatant using the cytotoxicity detection kit PLUS (Roche, Basel, Switzerland). 100 μl cell-free supernatant ...
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bioRxiv - Immunology 2024Quote: Cell death was analyzed by measuring LDH release into the cell culture medium with the Cytotoxicity Detection KitPLUS (LDH) kit (Roche, Cat# 04744926001) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Peptide N-glycosidase F (PNGaseF) was purchased from Roche Diagnostics (Mannheim ...
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bioRxiv - Neuroscience 2022Quote: ... and basic fibroblast growth factor (bFGF, 10 ng/ml; recombinant bovine, Roche). The cells were then plated in a 96-well plate in complete neurosphere medium containing DMEM/F-12 EGF and bFGF ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial pellet was resuspended in lysis buffer (binding buffer with protease inhibitors (Roche, ref ...
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bioRxiv - Bioengineering 2020Quote: Quantitative Polymerase Chain Reactions (qPCR) were prepared in 10μ reactions with SYBR Green I Master Mix (Roche), 1μL of genomic extract as a template ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The RBD fragment was amplified via polymerase chain reaction using Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) and then Gibson assembled in frame with an N-terminal His6-SUMO tag in the same vector as above ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using SYBR green PCR master mix (Kapa biosystems), forward and reverse primers (200 nM) ...
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bioRxiv - Biochemistry 2024Quote: ... excluding mitochondrial targeting sequence) were amplified by polymerase chain reaction (PCR) using Kapa HiFi Hotstart ReadyMix (Roche) and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase Chain Reactions (PCR) were performed using KAPA HiFi HotStart Ready Mix® (KAPA biosystems, Boston, USA) with the following thermal parameters ...
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bioRxiv - Genetics 2024Quote: ... TaqMan real-time polymerase chain reaction assays (PCR) consisted in 2.5 ul of Fast Master Mix (Roche), 0.125 ul of assay ...
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bioRxiv - Plant Biology 2024Quote: ... Gene expression was measured by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics) using the SYBR Premix Ex Taq (Tli RNaseH ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using the LightCycler 480 SYBR Green I Master (Roche, 04707516001) in a LightCycler 480 II (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in binding PBS buffer supplemented with Complete EDTA-free Protease inhibitor Cocktail (Roche) and lysed using an Emulsiflex-C5 homogeniser (Avestin) ...
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bioRxiv - Biochemistry 2022Quote: ... Hela nuclear extract (Ipracell Ltd) in binding buffer supplemented with protease inhibitors (Roche Ltd) was added to the immobilized proteins ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of heavy- and light chain pDNA were mixed with X-tremeGENE HP DNA Transfection Reagent (Roche) in FreeStyle™ 293 Expression Medium (Thermofisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was conducted on Roche Light cycler®96 (Roche Diagnostics, Mannheim, Germany) and amplification curve was checked with ROCHE software version 1.1 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using KAPA SYBR FAST qPCR Master Mix (KM4101, KAPA Biosystems, USA). The 2-ΔΔCT method was used for quantitative analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Humanization of the heavy (Vh) and light (Vl) chains of the best two candidates was performed by Roche using proprietary technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Immunology 2020Quote: ... and IgA) primers were used to amplify VH-region sequences using polymerase chain reaction (PCR high fidelity, Roche); separate PCR reactions for each VH-family were performed to avoid cross-priming or primer competition ...
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bioRxiv - Physiology 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green protocols (Kapa Biosystems, MA, USA) on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system) ...
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bioRxiv - Physiology 2023Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was carried out in a LightCycler 480 detection system (Roche) using the LightCycler FastStart DNA Master plus SYBR Green I kit (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (RT-qPCR) analyses were performed using a FastStart universal probe master (Roche) and StepOnePlus PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were considered not infected when no Cp value was calculated using Absolute quantification/2nd derivative Max analysis on the Light Cycler® 480 (Roche, Software release 1.5.1.62 SPS).
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bioRxiv - Microbiology 2022Quote: ... Measurement of viral DNA was performed using a quantitative polymerase chain reaction (qPCR) assay (Cobas 6800; Roche, Basel, Switzerland).
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to the manufacturer’s instructions and quantified by real-time quantitative polymerase chain reaction (RT-qPCR) performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Physiology 2023Quote: ... and quantitative real-time polymerase chain reaction (RT-PCR) was performed with SYBR Green protocols (Kapa Biosystems, MA, USA) and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative polymerase chain reactions (qPCRs) were performed using LIGHTCYCLER 480 SYBR GREEN I Master Mix (Roche, Indianapolis, IN, USA) in a Bio-Rad CFX384 Touch Real-Time PCR Detection System with 10 µL reaction volume ...
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bioRxiv - Immunology 2021Quote: ... Cultures were incubated with 400 µl of Lysis/Binding buffer (High Pure Isolation Kit; Roche) for 5 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... and via real-time quantitative polymerase chain reaction (qPCR) with the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). Final library pools were spiked with a non-indexed PhiX control library (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Biochemistry 2022Quote: ... I analyzed samples by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) using a Light Cycler 1.5 (Roche Diagnostics). Measurements form RNA of vascular cell adhesion molecule-1 (VCAM-1 ...
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bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription polymerase chain reaction (RT–PCR) was performed on the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Then cDNA was amplified using 2X Realstar Green Fast Mixture (Genstar) and a LightCycler 480 polymerase chain reaction system (Roche). All trials are performed in replicate reaction mixtures in 96-well plates ...
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bioRxiv - Genetics 2024Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with THUNDERBIRD® SYBR™ qPCR Mix (TOYOBO) using LightCycler®96 (Roche) and gene-specific primers described in Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were lysed with the Lysis Binding Buffer from the Magnapure LC Kit 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Neuroscience 2024Quote: ... The estimates for Aβ42 were then adjusted by a factor of 0.78 recommended by Roche specifically for this study sample ...
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bioRxiv - Plant Biology 2022Quote: ... and proteins were eluted by using 0.25 mg/ml HA peptide (Roche). HA- and FLAG-tagged proteins were immunologically detected using HRP-conjugated anti-HA 3F10 (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by 20 cycles of polymerase chain reaction (PCR) amplification using KAPA HiFi HotStart ReadyMix (#KK2602, Roche, Basel, Switzerland) and subsequent purification with Agencourt AMPure XP beads as described ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with the KAPA SYBR Fast qPCR kit (KAPA Biosystems Cat# KK4608) on a CFX96 Real-Time PCR Detection System (Bio-Rad RRID ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative polymerase chain reactions were performed using the Roche SYBER Green reagent and a LightCycler 480 apparatus (Roche Diagnostic, Meylan, France). Each cDNA sample was amplified in triplicate using SYBR Green (Roche ...
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bioRxiv - Bioengineering 2024Quote: ... Linear double-stranded DNA fragments were then amplified with Polymerase Chain Reaction (PCR) with KAPA HotStart ready mix (07958935001, Roche, Switzerland) using a reverse primer conjugated to biotin as previously described56 ...