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Citations for Roche :
1401 - 1450 of 1895 citations for Topoisomerase 2 alpha Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Biochemistry 2021Quote: Harvested cells were resuspended in lysis buffer (25 mM Tris pH 8.0, 150 mM NaCl, 2 μM Erlotinib and cOmplete Protease Inhibitor Cocktail (Roche)) and were lysed by sonication in pulse mode for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and sonicated in melanin buffer (50 mM Tris, 2 mM EDTA, and 150 mM NaCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Insoluble material was pelleted for 15 min at 16,000 g (4°C) ...
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bioRxiv - Developmental Biology 2020Quote: ... washed three times 15 min each in PBST and pre-incubated for 1h at 60°C in Hybridization Buffer (HB: 50% Formamide, 2X SSC, 1mg/ml Torula RNA, 0.05mg/ml, Heparin, 2% Roche blocking reagent ...
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bioRxiv - Microbiology 2021Quote: ... The PCR analysis was performed by mixing 5 μL of 1 μmol·L-1 of both primers and 2.5 μL of 0.25 ng·μL-1 template DNA with 12.5 μL 2× KAPA HiFi HotStart ReadyMix (Roche, Basel, Switzerland). The thermal program was 98°C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet was resuspended in 1-2 ml of lysis buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl; protease inhibitor [Roche]), depending on the size of the cell pellet ...
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bioRxiv - Developmental Biology 2020Quote: ... the tissue was minced and enzymatically digested in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001), 100 U/ml Hyaluronidase (Sigma #H3506) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were extracted with phosphate-buffered saline (PBS) containing 2 % Triton X-100 and protease inhibitors (Roche Complete ...
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bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). The solution was immediately desalted and the buffer exchanged by passing it over a Sephadex G25 (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... disulfide-bonded FimI at 4 °C 30-fold into 20 mM acetic acid-NaOH pH 5.0 containing a ~2-fold molar excess of FimC and complete EDTA-free protease inhibitor cocktail (Roche). FimCI was then dialyzed against 20 mM MOPS-NaOH pH 6.7 ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were prepared in lysis buffer (1% Triton, 1 mM DTT, 2 mM EDTA, 20 mM Tris pH 7.5 containing complete protease inhibitor, and PhosStop; Roche) from early (day 6-10 ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer2 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche), 0.5 % IGEPAL CA-630 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were lysed in lysis buffer (2% CHAPS, 50 mM HEPES, pH 7.4, 150 mM NaCl, 1mM Na3VO4, 1X EDTA-free PIC (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell pellet was dissolved in lysis buffer 2 (1x HEMG with 200 mM NaCl, 0.5% NP-40, 1x protease inhibitor cocktail (Roche), 0.5mM PMSF ...
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bioRxiv - Cancer Biology 2022Quote: ... and glands minced with surgical scissors before enzymatical dissociation for 1.5h in DMEM/F12 (1:1) supplemented with 2 mg mL−1 collagenase (Roche) + Gentamicin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM EDTA, 0.5% NP-40, 1 mM DTT, 1X protease inhibitor [EDTA-free complete Protease Inhibitor Cocktail, Roche, 11873580001] and 1:100 phosphatase inhibitor [Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 20 mM Tris-HCl (pH 7.4) with 2% SDS supplemented with a proteinase inhibitor (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Roche). Protein concentration of lysates was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: ... Pellets were further extracted by sonication in 3x volumes of SDS lysis buffer (50□mM Tris-HCl, pH 7.4, 2% SDS, 1X Complete Mini protease inhibitor cocktail [Roche]) with centrifugation at 21,000 x g for 30 min at 25°C to obtain the SDS-soluble fraction (Triton-insoluble) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1000 µL cold cytoplasmic lysis buffer (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 1000 µL cold sucrose buffer containing (10 mM HEPES pH 7.9, 10 mM KCl, 2 mM Mg acetate, 3 mM CaCl2, 340 mM sucrose, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA to 1× ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections from proximity labeling experiments were lysed and de-crosslinked in 100 mM Tris-HCl buffer (pH = 8.0) with 2% SDS and protease inhibitor (Roche) at 95 °C for 45 min with constant shaking ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in lysis buffer (150 mM NaCl, 50 mM HEPES pH 7.6, 1 mM EDTA, 2 mM MgCl2, 1% Triton X, 10% Glycerol, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 10% glycerol, 0.1% Triton X-100 and 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche]). Cell lysis was obtained by a mechanical-pestle and sonication ...
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bioRxiv - Synthetic Biology 2022Quote: ... Data from 2-3 technical replicates for all three biological replicates were analyzed in LightCycler® 96 software (Roche), Google Sheets ...
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bioRxiv - Cancer Biology 2022Quote: T cells were thawed into T-cell medium supplemented with 3,000 IU IL-2 (Proleukin) and DNAse (Pulmozyme, Roche) three to four days before coincubation with target cells ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cells were lysed with TNE-lysis buffer (50mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and cOmplete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in Laemmli buffer (62.5 mM Tris-HCl, pH 6.8, 10% glycerol, 2% SDS) supplemented with Complete® protease inhibitors (Roche), and sonicated before protein quantification (DC BioRad Protein Assay Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... skin tissues were homogenized at a 10% (w/v) concentration in a lysis buffer containing 2 mM CaCl2 and 0.25% (w/v) collagenase A (Roche) in Tris-Buffered Saline (TBS) ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured for the indicated amount of time with or without human IL-2 (TECINTM; teceleukin, ROCHE), human IL-15 (247-IL/CF ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell volume was determined again and 2 volumes cold buffer A++ (Buffer A + cOmplete protease inhibitors (Roche, cat 04693159001) and 0.15% NP40) ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2024Quote: ... After washes, the cells were stained with the DNA stain DAPI (4, 6 diamidino-2-phenylindole dihydrochloride) (Roche, #1023627001) and mounded with Prolong gold anti-fade mound media (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Successful virus rescue was monitored with SARS-CoV-2 Rapid Antigen Test (Cat# 9901-NCOV-01G, SD BIOSENSOR, Roche) by applying 100 μL of unfiltered culture supernatant directly onto the test device.
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were removed and pellets were re-suspended in 2% BSA/PBS containing RNase inhibitor (0.2 U/μL) (Roche). Nuclei were incubated with fluorescently conjugated antibodies against the neuronal marker NEUN (RBFOX3/NEUN (1B7 ...
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bioRxiv - Physiology 2023Quote: ... approximately 15 mg of pulverized mouse intestinal tissue was solubilized in ice-cold urea Lysis buffer (8 M urea in 50 mM Tris, pH 8.0, 40 mM NaCl, 2 mM MgCl2, 1x Roche cOmplete ULTRA EDTA-free protease inhibitor mini tablet ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Developmental Biology 2022Quote: ... 1% NP40, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1mM Na3VO4, 4 μg/ml leupeptin, one Roche cOmplete™ protease inhibitor tablet ...
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bioRxiv - Biochemistry 2023Quote: ... 0.75%/0.15% dodecyl maltoside/cholesteryl hemisuccinate (DDM/CHS) with 2 mg/ml iodoacetamide and a Protease Inhibitor tablet (Roche). After 4 hours ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... 100mM sodium chloride) for at least 2 hours and then incubated in anti-fluorescein-POD Fab fragments (Roche, 11207733910) diluted at 1:400 overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were resuspended in five pellet volumes of nucleolar isolation buffer (NIB: 10 mM Tris, 2 mM MgCl2, 0.5 mM EDTA) containing complete protease inhibitor (Roche). Samples were incubated in NIB buffer for 2 min at room temperature and then on ice for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and thus we dechorionated Stage 24-25 embryos (or 2 to 2.5 dpf) pupfish by incubating them on 20 mg/ml Pronase (Roche) in water for 2.5 hours ...
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl qPCR consisted of 10 µl 2× Light Cycle 480 SYBR Green I Master (Roche Applied Sciences), 1 µM each primer ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...