Mouse Anti Dengue Virus Membrane Protein Serotype 4 citations for Roche

Quote: ... Proteins were extracted by incubation with RIPA buffer and protease inhibitor cocktail (Roche, 4693159001) on ice for 1 hour with occasional vortexing ...

Integron Gene Cassettes Harboring Novel Variants of D-Alanine-D-Alanine Ligase Confer High-level Resistance to D-Cycloserine

Md. Ajijur Rahman, et al., bioRxiv - Microbiology 2020

Quote: ... pH 7.4) containing cOmplete EDTA-free protein inhibitor cocktail (Roche, 1 tablet/10 ml), lysozyme (1.0 mg/ml ...

Protein function achieved through multiple covalent states

Diego Butera, Philip J. Hogg, bioRxiv - Biochemistry 2019

Quote: ... washed and dried before digestion of proteins with 12.5 ng/μl of trypsin (Roche) in 25 mM NH4CO2 and 10 mM CaCl2 overnight at 25°C ...

A hidden gene in astroviruses encodes a cell-permeabilizing protein involved in virus release

Valeria Lulla, Andrew E. Firth, bioRxiv - Microbiology 2019

Quote: ... cells were lysed in 1% SDS containing PBS supplemented with protein inhibitor cocktail (Roche) and 250 U/ml bensonase (Sigma) ...

The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity

Jaehyoun Lee, et al., bioRxiv - Biochemistry 2020

Quote: ... Proteins were digested using Endoproteinase Lys-C at 1:100 w/w (Roche, 11058533103) at 37 °C overnight ...

Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

William Renthal, et al., bioRxiv - Neuroscience 2019

Quote: ... The protein lysates were extracted in presence of a protease cocktail tablet (Roche Diagnostics) using Cell Lysis buffer (ThermoFisher) ...

Extracellular vesicles as predictors of individual response to exercise training in youth living with obesity

Taiana M. Pierdoná, et al., bioRxiv - Physiology 2020

Quote: ... Proteins were extracted using 1:1 Pierce RIPA solution with protease inhibitor tablet (Roche). Samples were vortexed for 5 seconds ...

SARS-CoV-2 Spike Protein Interacts with Multiple Innate Immune Receptors

Chao Gao, et al., bioRxiv - Biochemistry 2020

Quote: ... MR-Fc was purified from cell culture supernatant using Protein A-agarose beads (Roche). For ELISA experiments ...

Network rewiring: physiological consequences of reciprocally exchanging the physical locations and growth-phase-dependent expression patterns of the Salmonella fis and dps genes

Marina M Bogue, et al., bioRxiv - Microbiology 2020

Quote: ... Antibody was removed by the addition of 100 µl homogeneous Protein G-Agarose (Roche) and a further incubation for 3 h ...

Cell- and non-cell-autonomous ARF3 coordinates meristem proliferation and organ patterning in Arabidopsis

Ke Zhang, et al., bioRxiv - Developmental Biology 2022

Quote: ... The lysate was precleared by incubation with 50 μL protein-A agarose beads (Roche) for 1 h and then incubated with anti-GFP (Abcam ...

In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer

Dzana Dervovic, et al., bioRxiv - Cancer Biology 2022

Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...

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