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Citations for Roche :
1401 - 1450 of 7885 citations for Anti M ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... on wet ice in cold 5 mM Tris-HCL (pH 7.5) with 0.32 M sucrose and protease and phosphatase inhibitor tablets (Complete Mini, EDTA-free and PhosSTOP tablets; Roche Diagnostics, Indianapolis, IN). Protein concentrations were obtained for each homogenate using a BCA protein assay kit (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Cell Biology 2021Quote: Total protein from cells or mouse tissues (n=3 per genotype) were extracted using the M-PER protein lysis buffer (ThermoScientific, Beverly, MA) containing protease inhibitors (Roche, Indianapolis, IN). Approximately 25 μg of total protein was electrophoresed on 4-12% SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with PBS and lysed (200 µl) with M-PER Mammalian Protein (ThermoScientific®) plus 1x Protease inhibitor (cOmplete Mini EDTA, Roche®). Cells were detached using a scraper (on ice) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared by scraping cells using 1X lysis buffer (10X recipe-50 mM Tris pH 7.5, Triton 20%, NP40 10%, 2 M NaCl, mixed with cOmplete protease inhibitor tablet - Roche, Product number 11873580001). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 13,000 rpm 4°C to pellet the cell debris ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting nuclear pellet was resuspended in wash buffer (10 mM Tris pH 8, 0.25 M sucrose, 10 mM MgCl2, 1 mM EDTA, 1% Triton X-100, 1X Roche protease inhibitor cocktail) and nuclei were cleaned and pelleted at 12,000 x g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed twice in 500 µl SPC buffer (1 M Sorbitol, 20 mM PIPES pH 6.3, 0.1 mM CaCl2, with Roche cOmplete protease inhibitors (Sigma)) and spun at 2k RPM for 3 minutes at 4°C between washes ...
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bioRxiv - Biophysics 2020Quote: ... Pellets were resuspended in lysis buffer (1 M NaCl, 20 mM Tris, 20 mM imidazole, Roche EDTA-free protease inhibitor, pH 7.5) and lysed by sonication ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with 12 ml of elution buffer (20 mM Tris, 500 mM NaCl, 6 M guanidine, 600 mM imidazole, 1 mM MgCl2 and 1X Roche protease inhibitor) and exchanged into #1 to #5 dialysis buffers in order to remove the imidazole and the Guanidine ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei pellets were resuspended in 100 µl of nuclei lysis buffer (20 mM HEPES pH 7.9, 0.1 M NaCl, 1mM EDTA, 1 mM EGTA, 1mM DTT, 25 % glycerol, 0.5 mM AEBSF, 1×Roche complete protease inhibitor cocktail) for 20 min at 4 °C with rotation ...
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bioRxiv - Genetics 2019Quote: ... Digoxigenin-labelled probes were detected with FITC-labelled antibodies (primary antibody from Roche, secondary antibodies from Jackson ImmunoResearch) ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody (Ki67) was diluted with Antibody Diluent (Reference: 760-108, Roche) and the samples were incubated 60 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-hemaglutinin (anti-HA; 12CA5; Roche) and PAP (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated for at least 1 h with a 1:1000 dilution of a high affinity anti-HA antibody from rat IgG1 (#11867423001, Roche Applied Science, Penzberg, Germany). Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795 ...
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bioRxiv - Developmental Biology 2020Quote: ... hybridized with sense or antisense probes overnight at 65 °C and incubated with the alkaline phosphatase-coupled sheep anti-digoxigenin antibody (1:2000, Roche Applied Science, Manheim, Germany) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: Ovaries and testis were fixed and stained according to standard procedures with the following primary antibodies: rat anti-HA (1:500, Roche cat# 11867423001, RRID: AB_390919), rat anti-Vasa (1:100 ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Developmental Biology 2022Quote: ... against foxg1 was carried out as previously described by Thisse (Thisse and Thisse, 2008) The antibody used to detect the riboprobes was AP-anti-DIG (Roche Cat.N: 11093274910, 1:500). For Fluorescent WM ISH (WM FISH) ...
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bioRxiv - Zoology 2024Quote: ... Samples were pre-incubated for 2 h in blocking solution (25% deactivated goat serum in PBT) and incubated overnight at 4°C in blocking solution with anti-DIG antibody conjugated to alkaline phosphatase (Roche Diagnostics, Germany; 1:2000). After several washes in PBT ...
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bioRxiv - Plant Biology 2019Quote: ... The fine powder was resuspended with the extraction buffer (50 mm Tris, pH 8, 8 M urea, and 0.5% SDS) supplemented with protease inhibitor (Roche Diagnostics GmbH, Mannheim, Germany), and homogenized with a Dounce homogenizer ...
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bioRxiv - Biochemistry 2020Quote: ... The spheroplasts were resuspended in ice-cold D-buffer (0.6 M sorbitol, 10 mM Tris-HCl pH 7.4) containing protease inhibitors (complete protease inhibitor cocktail, Roche; 1 tablet per 50 mL), and lysed by 10 strokes in a Dounce homogenizer ...
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bioRxiv - Neuroscience 2022Quote: ... They were scooped with a brush and dropped in a glass tube containing the homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) at 4°C (50 μl of buffer for each slice) ...
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bioRxiv - Neuroscience 2020Quote: Genome-wide methylation analysis was performed using the Roche SeqCap Epi Developer M Enrichment system on bisulfite-treated DNA (Roche Nimblegen; WI, USA), with custom designed primers targeting promoter and enhancer regions of the genome ...
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bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the dishes were placed on ice and 500 μl of homogenization buffer (HB; 0.32 M sucrose and one tablet Complete mini EDTA-free protease inhibitor cocktail (Roche; 10 ml, pH 7.4)) (4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-sense-tiRNA DNA oligos were ordered from IDT and labelled with DIG using the DIG Oligonucleotide Tailing Kit (Roche, 03353583910). Sequences of the probes are ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibodies: mouse α-GFP (Roche), rabbit α-RFP (Rockland) ...
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bioRxiv - Developmental Biology 2020Quote: ... alkaline phosphatase-conjugated antibody (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... after which DIG antibody (Roche) was added to the blocking buffer at a ratio of 1:10,000 and incubated for an additional 1/2 min at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... antibodies and NBT/BCIP (Roche) or INT/BCIP (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Commercial primary antibodies: EGFP (Roche), Myc (9E10 ...
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bioRxiv - Cell Biology 2022Quote: ... GFP antibody (Cat#11814460001 Roche), IKKε Rabbit Ab (#2905S ...
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bioRxiv - Cell Biology 2022Quote: ... DIG-AP-antibody (11093274910 Roche) incubation was then performed overnight at 4 °C (1:2000 in blocking solution) ...
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bioRxiv - Neuroscience 2021Quote: ... GFP mouse antibody (Roche #11814460001), KIF13A rabbit antibody (Thermo Fisher Scientific PA5-30874) ...
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bioRxiv - Biochemistry 2022Quote: ... antibodies against DIG (Roche 11333089001) or tubulin (TU-20 ...
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bioRxiv - Neuroscience 2024Quote: ... alkaline phosphatase-conjugated antibody (Roche). The sections were subsequently rinsed and incubated in the dark with a NBT/BCIP solution (Nitroblue tetrazolium chloride/ 5-bromo-4chloro-3-indyl-phosphate ...
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bioRxiv - Cell Biology 2024Quote: ... or α-HA antibodies (Roche).
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bioRxiv - Cell Biology 2024Quote: ... or α-HA antibodies (Roche).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies (α-HA3F10 Roche were incubated overnight at 4°C in PBS containing 1% BSA and 0.02% sodium azide ...
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bioRxiv - Developmental Biology 2023Quote: ... Fab fragments antibody (Roche, 11426339810) was added (1:2000 dilution in PBT + 2 mg/ml BSA + 2% sheep serum ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies against Myc (9E10; Roche), 14-3-3 (H-8 ...
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801; HA: Roche #11867423001; VSVG: Sigma #SAB4200695) in a total volume of 500 μL 50 mM MES (pH 5.0) ...
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bioRxiv - Genomics 2022Quote: ... Positivity of SARS-CoV-2 infection was assessed both by PCR and measurement of specific antibodies (Cobas-Roche using Elecsys Anti-SARS-CoV-2 S). All patients gave their consent for participation in this study ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-AtSMC3 and anti-GFP (Roche 11814460001) antibodies were diluted 1:500 and 1:1000 in PBST milk 5 % ...
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bioRxiv - Genetics 2020Quote: ... 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml, ½ Roche phosSTOP tablet/10 ml) using 14 × 1.5 min bursts on a BioSpec mini bead-beater at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... pooled together and placed in buffer H (10 mM HEPES pH 7.5, 5 mM MgCl2, 25 mM KCl, 0.25 M sucrose supplemented with Complete protease inhibitors cocktail, Roche Products Ltd, Welwyn Garden City, UK) at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 300 mM NaCl, 7.5 mM MgCl2, 0.25 mM EDTA, 1 mM DTT, 1 M Urea, 1 % NP-40, 1x Roche complete, 20 U/mL RNase Out), pipetting up and down 30 times ...
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bioRxiv - Genomics 2020Quote: ... Chemiluminescent detection of anti-DIG was performed using CDP-Star reagents from the DIG Northern Starter Kit (Roche # 12 039 672 910). Densitometry was performed in ImageJ.
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bioRxiv - Genetics 2021Quote: ... The T7 promoter was added to the 5’ and 3’ ends to synthesize the sense and anti-sense probes using the DIG RNA Labeling Kit (Roche, Cat# 11175025910). In situ hybridization was performed as previously described [65,66] with minor modifications ...