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Citations for Roche :
1401 - 1450 of 9189 citations for 8 2 5 dimethyl 4 chlorophenylsulfonamido 1 naphthol 3 6 disulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and 0.1% Tween 20) for 5 min each and then incubated in the AP reaction buffer with NBT/BCIP substrate (Roche).
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Eight million HEK293T cells were lysed with 800 μL cell lysis buffer (HBS, 0.1% NP-40, 5 mM EGTA, supplemented with 1 χ protease inhibitor cocktail [Roche, Cat.# 11873580001] ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear pellets were lysed by sonication on ice in 10 mL lysis buffer (50 mM Tris pH 7.4, 0.5 M NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, 1x Roche complete protease inhibitor). After sonication ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
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bioRxiv - Neuroscience 2019Quote: ... and hybridized overnight at 55°C with 1 ng/ml digoxigenin-labelled (GGGGCC)5 oligonucleotide probe (Integrated DNA Technologies) in 25 ml hybridization reaction (EasyHyb Granules, Roche). Following denaturation of the probe at 95°C for 5 min ...
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bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
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bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Genomics 2022Quote: ... Pellets were harvested after 16-18h and resuspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 tablets per 50 ml protease inhibitor (EDTA-free, Roche), 0.2 mM PMSF ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol and protease inhibitor cocktail (cOmplete, EDTA-free; Roche) in 50 mM Tris HCl ...
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bioRxiv - Neuroscience 2019Quote: ... cells were lysed in Hepes buffer (20 mM Hepes, 1% CHAPS, 100 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail III (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: ... were lysed with lysis buffer (25mM Tris, 150mM NaCl, 1mM EDTA, 1% NP-40, 5% Glyceral) with proteinase inhibitor cocktail (Roche) on ice for 30 min ...
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bioRxiv - Immunology 2019Quote: A single-cell suspension of lung cells was obtained by mincing whole mouse lungs in RPMI and then digesting each lung in 5 mL of RPMI + 10% FCS with 1 mg/mL Collagenase A (Roche) and 2000 U/mL of DNAse I for 45 minutes in a shaking incubator at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5% glycerol with the addition of 1 EDTA-free cOmplete protease inhibitor cocktail tablet per 50 mL lysate (Roche). Cells were lysed by sonication on ice with stirring and the lysates were clarified by centrifugation at 20,000 g for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... digested using 0.1 % papain at 37°C for 10 min and for 5 additional minutes in presence of DNAse1 (1 mg/ml, Roche). After stopping papain activity by adding MC+ media ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM NaCl, 50 mM sodium pyrophosphate, 50 mM sodium fluoride, 1% Triton-X-100, 10% glycerol, 5 mM EDTA, Roche MiniProtease Inhibitor cocktail tablet ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with SAE2-T2A-SAE1 cDNA in the pcDNA5/FRT/TO vector and the Flp-recombinase cDNA in the pOG44 vector at a 5:1 pcDNA5/FRT/TO DNA: pOGG44 DNA ratio using FuGene6 (Roche) at a ratio of 4:1 FuGene (μl) ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
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bioRxiv - Neuroscience 2023Quote: ... Five P2 mouse brains were homogenized in 5 mL of homogenization buffer (5 mM Tris-HCl, 0.32 M sucrose, 1 mM MgCl2) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche), then centrifuged at 1,660 x g for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: Tissues from animals were homogenized and lysed in HED buffer (25 mM HEPES, 5 mM EDTA, 10% glycerol, 1 mM DTT, and 1x cOmplete protease inhibitor [Roche]). Lysates were sonicated for 20 minutes in a water bath sonicator and cleared by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, incubated in a hybridization buffer [(50% formamide, 5× SSPE, 0.1% SDS, and 1 mg/ml Yeast tRNA (Roche Diagnostics)] containing the Avidin solution (Vector Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001]). 40 µg of protein was loaded onto NuPAGE-Novex Bis-Tris 4%–12% gradient gels (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Immunology 2024Quote: ... LNs obtained from organ donors were submerged in 5 mL R10 media (RPMI + 10% FBS + 1% penicillin/streptomycin + 2mM L-glutamine) supplemented with 10U/mL DNase I (Roche), cleaned of visceral fat ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 x Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The protein concentration was determined by BCA assay (23225 ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 2 mM MgCl2, 1% Triton X-100, 50 mM HEPES pH 7.4, 1 × Protease Inhibitor Cocktail [04693159001, Roche Diagnostics], 50 mM NaF, 0.2 mM Na3VO4). The remaining (87% ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Cell Biology 2021Quote: ... and 3× EDTA-free complete protease inhibitor cocktail (Roche). Lysates were briefly sonicated until the protein solution was clear ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with blocking buffer containing 3 % BSA (Roche Diagnostics), 5 % goat serum (Jackson ImmunoResearch Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: Clonazepam (CAS #1622-61-3) was bought from Roche on 2018 ...
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bioRxiv - Biophysics 2024Quote: ... 20 mM imidazole) supplemented with 3 protease inhibitors (Roche) and then lysed by an EmulsiFlex-C3 (Avestin ...
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bioRxiv - Biochemistry 2023Quote: ... 3 cOmplete EDTA-free protease inhibitor Cocktail tablets (Roche), pH 7.2 ...
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bioRxiv - Genetics 2020Quote: ... 0.25% deoxycholic acid) plus protease inhibitor and phosphatase inhibitor Data Setts (Roche Applied Science). 11 μg protein was separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and free fatty acids in plasma was performed using commercial kits (Biovision, Roche, Cayman). Mouse plasma lipoprotein profiles were established by TNO Biosciences (Leiden ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with phenylmethanesulfonyl fluoride solution and a protease inhibitor cocktail with ethylenediaminetetraacetic acid (Roche). Brain tissue was homogenized in ice-cold RIPA buffer (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated (nucleic acid purification on the MagNA Pure 96; Roche Life Science), reverse transcribed and Taqman PCR (on the 7500 RealTime PCR system ...
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bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from samples using Magnapure LC total nucleic acid isolation kit (Roche). RNA amplification and quantification were carried out using a 7500 Real-Time PCR System (Applied biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... NEFA were analysed using the Free Fatty Acid Kit (half-micro test) (11383175001, Roche) and TG was measured using an enzymatic assay (DF69A ...
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bioRxiv - Paleontology 2019Quote: ... but adapted for the use of High Pure Nucleic Acid Large Volume columns (Roche) instead of the Zymo-Spin V column (Zymo Research ...