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Citations for Roche :
1351 - 1400 of 10000+ citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 1 WFIKKN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
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bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
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bioRxiv - Immunology 2020Quote: ... The proliferation rate of lymphocytes was measured using 5-Bromo-2-deoxyuridine (BrDU) assay as per the manufacturer instructions (Cell Proliferation ELISA BrDU, Roche, USA).
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bioRxiv - Biochemistry 2019Quote: ... Fisher Bioreagents) (PBS-T) and re-suspended in 50 μl storage buffer (Blocking Reagent for ELISA, 11 112 589 001, Roche Diagnostics) supplemented with ProClin (4812-U ...
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bioRxiv - Microbiology 2020Quote: IL-6 release over time was studied in the Laboratoriumsmedizin of the Munich University using an ELISA (Roche, Elecsys IL-6).
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bioRxiv - Molecular Biology 2021Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus ...
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bioRxiv - Molecular Biology 2020Quote: ... the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Roche) for 2 hours at 30 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G agarose beads (Roche #11719416001) pre-blocked with salmon sperm (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... together with protein A agarose (Roche) overnight at 4 ℃ ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein G agarose beads (11243233001, Roche) were added to the lysates and incubated for additional 4 hours at 4°C with rotary agitation ...
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bioRxiv - Neuroscience 2021Quote: ... homogeneous protein G-agarose suspension (Roche) was added to the suspension ...
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bioRxiv - Microbiology 2019Quote: ... Protein G-conjugated agarose beads (Roche) or PrecipHen for chicken antibodies (Aves Labs ...
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bioRxiv - Cell Biology 2019Quote: ... protein inhibitor cocktail (Roche, Mannheim, Germany) and then sonicated ...
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bioRxiv - Genetics 2021Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2022Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Developmental Biology 2023Quote: ... and Protein G-Agarose (Roche, 11243233001) were added to each sample and incubated for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested with LysC (Roche, Basel ...
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bioRxiv - Cancer Biology 2019Quote: ... Pyrosequencing PCR was performed in duplicate for each sample in a 25 μl volume containing an end-concentration of 1 U Faststart Taq polymerase (Roche, Welwyn Garden City, UK), 1x FastStart PCR Buffer including 2 μM MgCl2 (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: Tissue or cells were washed with cold PBS and lysed in 1% NP40 lysis buffer containing a protease and phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN, USA). Proteins were analyzed by SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... DIGprobes were detected with antibodies in MABT containing 5% horse serum appropriate for NBT/BCIP in situ hybridization (Roche anti-DIG-AP 1:1000) or for FISH (Roche anti-DIG-POD 1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... whereas mouse primary cortical cultures and activated T lymphocytes were lysed in lysis buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100) containing protease and phosphatase inhibitors (Roche, New York, NY, USA). Total protein contents were quantified using the BCA assay (Pierce) ...
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bioRxiv - Physiology 2022Quote: ... This was followed by a perfusion at 4 mL/min for 40 min with the same solution containing 1 mg/mL of collagenase A (Roche Diagnostics GmbH, Mannheim, Germany) plus 300 µM ethylene glycol tetraacetic acid (EGTA ...
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bioRxiv - Plant Biology 2019Quote: ... 300 mM sucrose, 1% Triton X-100) containing 1x protease inhibitor cocktail (Pierce, Rockford, IL) and a phosphatase inhibitor (PhosSTOP, Roche Applied Science, Penzberg, Germany). Protein extracts were centrifuged at 1,500 rpm for 5 min and the supernatant was re-centrifuged at 12,000 rpm for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and homogenized in 5 volumes of ice-cold PBS containing 1% NP-40 and Complete® protease inhibitor cocktail tablets (Roche Diagnostics, Basel, Switzerland) using 2×10 clockwise strokes with 5 s rest time ...
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bioRxiv - Biochemistry 2023Quote: ... and were resuspended in 30 mL lysis buffer 1 (50 mM sodium phosphate, 300 mM NaCl, pH 8) containing 2 mL of EDTA-free protease inhibitor mixture (Roche Applied Science, Penzberg, Germany). Cells were disrupted by passing the cell suspension three times through a Constant Cell disruption system (TS benchtop ...
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bioRxiv - Cell Biology 2020Quote: ... bovine serum albumin in TBS-T and incubated for 16 h at 4°C: mouse anti-GFP (1:1000) (Roche), sheep anti-CK1α (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were then probed for 16 h at 4 °C with the following primary antibodies in 3% (w/v) skim milk in PBS: mouse anti-GFP (1:1000, Roche), rabbit anti-SBP1 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were in contact for 2 hours at room temperature with a mouse monoclonal primary antibody anti-BrdU (1/1000 dilution) (Roche), then rinsed and incubated for 30 min with a fluorescent secondary antibody Alexa-633nm goat anti-mouse (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was then washed and incubated 1 hs at RT with goat anti-mouse secondary antibody conjugated to HRP (Roche) at 1/20000 dilution in PBST - 5% milk.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 4°C overnight with the primary antibody mouse IgG1 Anti-Tbx6 (home-made, clone A83, dilution 1:500) in 2% Blocking Reagent (Roche) and 5% sheep serum (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were blocked in 5% low-fat milk in TBS-T for 2 hours and probed with 1:5000-diluted mouse anti-GFP (Roche) or rabbit anti-ATG8 (Agrisera ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Cancer Biology 2019Quote: ... and 1-3 ng of ChIP DNA with KAPA Library Preparation Kit (KAPA Biosystems) and NimbleGen SeqCap Adaptor Kit A or B (Roche ...
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bioRxiv - Microbiology 2020Quote: Biotinylated HIV-1 FISH probes were prepared with the Nick Translation Kit (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 20 µg mL-1 luciferase reagent (ATP Bioluminescence Assay Kit CLS-II, Roche). The reaction was initiated with 200 µM NADH ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Cancer Biology 2022Quote: For antibody incubation 5 µl of the DigiWest Bead mixes were added to 50 µl assay buffer (Blocking Reagent for ELISA (Roche, Rotkreuz, Switzerland) supplemented with 0.2% milk powder ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1% SDS) containing a protease inhibitor cocktail (Roche, 11697498001). Equal amounts of protein were separated on 10%-15% SDS-PAGE gels and transferred to PVDF membranes (Roche ...
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bioRxiv - Microbiology 2019Quote: ... and homogenized in RIPA containing cOmplete Protease Inhibitor Cocktail (Roche), denatured in Laemmli buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% sodium deoxycholate) containing a protease inhibitor cocktail (Roche, 11697498001). Protein samples were separated by SDS-PAGE and blotted onto the PVDF membranes ...
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bioRxiv - Developmental Biology 2020Quote: ... containing cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche, USA) and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5% Triton X-100 containing phosphatases and proteases inhibitors (Roche). For detection of the Huntingtin protein ...