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Citations for Roche :
1351 - 1400 of 2903 citations for 6 Chloro 9 2 C methyl β D riboFuranosyl 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... ∼200-300 embryos were dechorionated 3-6 hours after injection by 5 min incubation in 1 mg/ml pronase (Roche) in E3 medium in a 2% agarose-coated petri dish and washed with excess amount of fresh E3 ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates were prepared following euthanasia of zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Complete ULTRA Tablets, Roche), followed by homogenisation via probe sonication ...
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bioRxiv - Bioengineering 2020Quote: ... Hela cells were plated one day before in 6-well plate at 50% confluency and transfected with 1 μg plasmid and 3 μl XtremeGeneHP transfection reagent (Roche) during 24 hours ...
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bioRxiv - Immunology 2020Quote: ... SHIV-Ag85B was prepared by transfecting pSHIV-Ag85B into 293T cells using FuGENE 6 Transfection Reagent (Roche Diagnostics, Indianapolis, IN), and the culture supernatant was stored at 48 h after transfection in liquid nitrogen until use.
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was set by using qPCRBIO Probe Mix Hi-ROX (Nippongenetics) and TaqMan (5’: 6-FAM, 3’: TAMRA) or UPL (Universal Probe Library, Roche) probes ...
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bioRxiv - Cancer Biology 2022Quote: A vector control plasmid and WT and mutant Plk3 expression plasmids were transfected into 293T cells using FuGENE 6 transfection reagent (Roche) as described previously (Li et al. ...
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bioRxiv - Plant Biology 2022Quote: ... Glucose was then quantified by measuring NADH production using an assay based on hexokinase and glucose-6-phosphate dehydrogenase (Roche). Starch content was calculated in glucose equivalents.
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bioRxiv - Developmental Biology 2022Quote: ... bleached with 6% hydrogen peroxide in PBS for 1h and treated with 10 μg/ml Proteinase K (Roche, Mannheim, Germany) for 20 min (5 min for epithelia ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and then detected with 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) substrate (Roche Diagnostics) diluted at 1:1000 in H2O2 ...
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bioRxiv - Cell Biology 2023Quote: ... D-glucose was measured spectrophotometrically3 by reading the increase in NADPH(H+) absorbance at 340 nm produced in two coupled reactions catalyzed by hexokinase and glucose-6-phosphate dehydrogenase (G6PD) (Roche diagnostics Corporation ...
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bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Then the different pellets were resuspended for 30 min in denaturing buffer (20 mM Tris, 500 mM NaCl, 6 M Guanidine, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... values of corresponding cDNAs with the QuantStudio 6 Pro system (Applied Biosciences, A43180) and FastStart Essential DNA Green Master (Roche) at a final concentration of 1X ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded at 400,000 cells per well in 6-well plates and transfected the day after with indicated cDNA constructs with FuGENE6 (Roche). Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded 72 hours prior to the experiment at 400,000 cells per well in 6-well plates and transfected the next day with wt or mutated myc-ALPK1 constructs with FuGENE6 (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Proteins were harvested from ∼30 mg of tissue (in vivo) or two 6-well plates (in vitro) and homogenised in Ripa buffer with complete mini-proteinase inhibitors (Roche). Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Pathology 2023Quote: ... in HEK 293T cells (3×105 cells per well in 6-well plates) using X-treme gene transfection reagent (Roche). Pseudotyping was achieved by co-transfecting pHEF-VSVg (400 ng/well) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were amplified by ligation-mediated PCR for 6 cycles using a KAPA HiFi HotStart high-fidelity DNA polymerase (Roche) and purified using AMPure XP beads.
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bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed 6 times with MABT and were then incubated with anti-DIG AP-conjugated Fab fragment (Roche, 11093274910), Alexa-Fluor 488 goat anti-Mouse IgG1 antibody (ThermoFisher A-21121 ...
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bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
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bioRxiv - Microbiology 2024Quote: ... spleens were collected from naïve C57BL/6 (CD45.1+) and a single cell suspension was obtained by incubation with Liberase Blendzyme 3 (Roche, Bradford, CT) and DNase (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... PDOs were pooled from 6 wells and incubated for 1.5 hours on ice in Cell Recovery Buffer supplemented with phosphatase inhibitors (Roche, 4906845001). PDOs were lysed using RIPA buffer (Boston BioProducts ...
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bioRxiv - Biochemistry 2021Quote: Cardiac myofibrils were prepared from left ventricular trabecular strips pre-stretched overnight to a sarcomere length of about 2 µm in rigor buffer (20 mM HEPES pH 7, 140 mM KCl, 2 mM MgCl2, 2 mM EGTA, 1 mM DTT, Roche complete protease inhibitor) at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Reverse crosslinking was performed overnight at 65>°C with high NaCl and RNase treatment (Roche). Proteinase K (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The Micro-C library was generated by using the KAPA HiFi HotStart ReadyMix (Roche #KK2601) or the Q5 High-Fidelity 2X Master Mix (NEB #E7645 ...
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bioRxiv - Genomics 2019Quote: All buffers were pre-chilled to 4°C with cOmplete EDTA-free protease inhibitor (Roche) freshly added ...
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP ...
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bioRxiv - Systems Biology 2021Quote: ... epidermal sheets were digested in Liberase Tm (13 U/ml, Roche, UK, 2h, +37°C,) and enriched using density gradient centrifugation (Optiprep 1:4.2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated overnight at 4 °C with rat anti-HA antibody (1:500 - Roche) and then with secondary goat anti-rat IgG antibody coupled to Alexa 568 (Invitrogen/Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 10 min at 37°C with anti-HA antibody (3F10; Roche) (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... minced and enzymatically dissociated at 37°C for 45 min in 0.2% collagenase A (Roche) and 1 mg/ml dispase (GibcoBRL) ...
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bioRxiv - Molecular Biology 2020Quote: HDL and LDL measurements were done using the Cobas C-111 chemistry analyzer (Roche, Switzerland).
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bioRxiv - Genomics 2021Quote: ... The membrane was hybridised overnight at 42 °C with DIG Easy Hyb (Roche®, 11603558001). The DNA was detected with anti-digoxigenin-Alkaline Phosphatase antibody (Roche® ...
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bioRxiv - Molecular Biology 2022Quote: ... 72 °C for 10 s) using KAPA Hifi Hot Start Ready Mix (KAPA Biosystems KK2602) and primers (fw ...
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed at 4°C in RIPA buffer supplemented with protease inhibitors (Roche, 4693159001). Protein concentrations were measured using a BCA Assay Kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2022Quote: ... S195A-GD-FXa protein was further purified on an anti-Protein C Affinity matrix (Roche). Maldi mass spectrometry showed that TFPI was glycosylated ...
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bioRxiv - Developmental Biology 2022Quote: ... cv-c riboprobe was marked using DIG RNA Labelling Kit (Roche, 11 175 025 910). Images were taken on an SPE Leica confocal microscope and processed using FIJI and Adobe Photoshop programs.
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bioRxiv - Microbiology 2023Quote: ... and treated for 30 min at 37°C with 20 U/ml DNase I (Roche). Virus was pelleted through a 25% sucrose cushion ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was pre-hybridized and hybridized at 50°C in DIG eazy Hyb (Roche). Two double washing steps were realized using 0.1% SDS ...
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bioRxiv - Developmental Biology 2023Quote: ... reverse crosslinked by incubation overnight at 65°C in the presence of proteinase K (Roche), and cleaned by RNase A (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... diluted in 100 mM ammonium acetate (pH = 4) and digested with Glu-C protease (Roche) at 10:1 protein:enzyme for 1 h at room temperature prior to mass spectrometric analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... 1xSSC and 0.5xSSC at 55°C followed by anti-Digoxigenin antibody (Roche #11093274910, 1:500) incubation for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and digested for 1 h at 37°C in 94 µg/mL DNase I (Roche) and either 250 µg/mL Liberase DL (Sigma-Aldrich ...