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Citations for Roche :
1301 - 1350 of 5641 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... A total of 50 ng DNase-treated RNA was used as template for reverse transcription using either RT Transcriptor from Roche and random hexamers or Moloney Murine Leukemia Virus-RT (MMULV-RT ...
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bioRxiv - Cell Biology 2019Quote: To generate a peptide spanning lysin 105 and 106 the immunoprecipitates were digested in solution with 400 ng Glu-C (Sequencing Grade, Roche) at 37C for 16 hours.
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bioRxiv - Developmental Biology 2020Quote: ... a final probe concentration of 0.1 ng/μL was used and the probe was detected using an alkaline phosphatase conjugated anti-digoxigenin antibody (1:1500; Roche) and NBT/BCIP (Roche ...
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bioRxiv - Genomics 2019Quote: ... 5 ng DNA fragments were pooled and proceeded on with library construction using KAPA Hyper Prep Kit (Kapa Biosystems #KK8504) according to the standard protocol ...
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bioRxiv - Genomics 2019Quote: ... To remove RNA in the RNA:cDNA duplex the sample was incubated for one hour at 37° C with 125 mU/µl RNase H (Y9220F, Enzymatics) and 500 ng/µl RNase A (11579681001, Roche) in 1 × Rnase H buffer (Y9220F ...
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bioRxiv - Microbiology 2019Quote: ... in a final volume of 25 μl containing 12.5 ng of genomic DNA and 2X KAPA HiFi HotStart ReadyMix (Kapa Biosystems). PCR reactions were performed in a Thermal Cycle T gradient (Biometra GmbH ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... One to five ng of DNA was analyzed by qPCR using 300 nM primers and SYBRgreen Master mix (Roche AG) using a QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were prepared by the Van Andel Genomics Core from 300 ng of total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16; Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Cell Biology 2021Quote: ... The qPCR was performed using 20 ng of cDNA product per reaction in the presence of Lightcycler 480 SYBR green dye (Roche). Lysosomal gene primers were designed using PrimerBLAST and ordered from Eurofins ...
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bioRxiv - Plant Biology 2021Quote: ... Quantification of transcript levels was carried out using SYBR Green reactions with 5 ng cDNA in a 20 μl volume on a Light Cycler 480 II (Roche) relative to the reference gene UBC10 (POLYUBIQUITIN10 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 200-500 ng of total RNA in the Transcriptor First Strand cDNA synthesis kit (Roche) with 60 μM random hexamer and 6.25 μM oligo(dT)18 primer ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 250-800 ng DNA per well of a 12-well plate or 500 ng per well of a 6-well plate using X-tremeGENE 9 DNA transfection reagent (Roche), and harvested after 24 h.
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bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative reverse transcription PCR (RT-PCR) was performed with 10 ng of cDNA in a Light Cycler® Instrument (Roche) using LightCycler® 480 probes master mix (4887301001 ...
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bioRxiv - Genetics 2022Quote: ... were prepared and sequenced by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA mRNA HyperPrep kit (v4.17) (Kapa Biosystems). RNA was sheared to 300-400 bp ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of DNA was subjected to PCR amplification using the Kapa HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, USA). The individual PCR reactions were performed in 20 μL final volume and contained:
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bioRxiv - Microbiology 2021Quote: ... Between 150 and 200 ng of genomic DNA was sheared acoustically and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). PCR amplification of the libraries was carried out for 10 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... Each DNA sample was diluted to 1 ng/μL and 2 μL were used for amplification using PrimeTime Gene expression Master Mix (IDT) with LightCycler96 (Roche) instrument ...
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bioRxiv - Genomics 2019Quote: ... Amplified genomic DNA (70 ng) was used to generate exome sequence libraries using the Hyper Prep kit (Kapa Biosystems, KK8504), SureSelect Target Enrichment kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time quantitative PCR reactions from 8,3 ng of cDNA were set up in triplicate using a LightCycler DNA SYBR Green I Master PCR machine (Roche). The oligonucleotides used in qRT-PCR experiments are provided in SI6.
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bioRxiv - Developmental Biology 2020Quote: ... 500 ng DNA was subjected to in vitro transcription with T3 polymerase and DIG- or FITC-labeled ribonucleotides (All Roche) for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of 25 × diluted oligonucleotides and 50 ng of the digested and dephosphorylated vector were ligated overnight at 16°C using T4 Ligase (Roche), and transformed into XL10-Gold E ...
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bioRxiv - Immunology 2020Quote: ... 2.7 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK 8504). Samples were barcoded and used for 50bp/50bp paired end runs with the TruSeq SBS Kit v4 (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: ... Nucleosomal DNA was isolated and either used as a template for qPCR (ChIP-qPCR) or to generate NGS libraries using KAPA Hyper Prep Kits (Roche) and TruSeq adaptors (Bioo Scientific ...
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bioRxiv - Genomics 2021Quote: ... Both libraries were quality controlled using a Fragment analyzer NGS kit (AATI) and qPCR with the Kapa library quantification kit (Roche) prior to sequencing.
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bioRxiv - Cell Biology 2021Quote: ... A volume corresponding to 25-50 ng of cDNA was used for each RT-qPCR reaction using Roche LightCycler 480 SYBR Green I Master (04707516001, Roche) sequence detection system ...
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bioRxiv - Cancer Biology 2022Quote: ... Validation of the library was performed on Fragment Analyzer (Standard Sensitivity NGS kit) and using qPCR (ROCHE Light Cycler480, France). Total RNAs were sequenced on NovaSeq 6000 from Illumina ...
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bioRxiv - Biochemistry 2022Quote: ... 150 ng of U1 RNA (TMG or NAD cap) and 150 ng of HIV-1 mRNA were mixed with ResoLight dye (1x, Roche) and annealing buffer (10 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.05% Tween 20) before exchange of the pre-incubation solution for hybridization buffer containing 3 ng/µl of digoxigenin-labeled (Roche) riboprobe ...
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bioRxiv - Cancer Biology 2022Quote: ... and 500 ng of DNA-free RNA was reverse transcribed using the First Strand cDNA Transcriptor Synthesis Kit (Roche Life Science, Roche Diagnostics Division ...
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Molecular Biology 2022Quote: ... Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche, 6366236001), at a ratio of 400 ng DNA to 40 µL 37 °C serum-free DMEM to 1.6 µL reagent per dish being transfected ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA (150-200 ng) was sheared and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). Libraries were amplified by PCR (10 cycles) ...
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bioRxiv - Plant Biology 2023Quote: ... The real-time qPCR reactions were performed from 10 ng of cDNA using LightCycler 480 SYBR Green I master mix (Roche) in 96-well plates according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... 20 ng of synthesized cDNA were loaded in the qPCR reactions using qPCR Master Mix (Fast start Sybr green Master; Roche) in QuantStudio 3 (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using ∼50 ng of mRNA using the Kapa Stranded RNA-Seq Library Preparation Kit (KAPA Biosystems, Illumina). Indexes were designed according to TruSeq RNA UD specifications (#20020591 ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng genomic DNA was used as a template for PCR amplification with KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2602) and primer pairs containing an internal locus-specific region and an outer Illumina-compatible adaptor sequence ...
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bioRxiv - Microbiology 2022Quote: ... The total qPCR reaction volume was 1.5 □μL with 0.5□ μl of DNA (40□ng□μl-1) and 1□μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5□μM of PCR primers ...
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing libraries were prepared with 200 ng of total RNA using KAPA RiboErase Stranded RNA-Seq kit (cat # KK8483) following standard protocol (Roche). Sequencing was done at Stanford Functional Genomics Facility (Stanford ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ng of cDNA were amplified per reaction and each reaction was performed in triplicate using the LightCycler 384 (Roche) with SYBR Green Master Mix II (Roche) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and library preparation was carried out using the Accel-NGS 2S Plus DNA Library Kit (Swift Bioscience) and amplified with the KAPA HiFi DNA polymerase (Roche). The amplified library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... One hundred ng of genomic DNA was used for PCR amplification of the target loci using KAPA HiFi HotStart PCR kit (Roche) and the primer sets in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 500 ng of RNA were then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche LifeScience) as previously described (Montañés et al. ...
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bioRxiv - Microbiology 2024Quote: ... Transposon junctions were amplified with oligos pJZ_Fwd_RND1 and olj376 using 500 ng DNA and KAPA HiFi Hotstart Mix (Kapa Biosystems, #KK2602). Reactions were stopped at the inflection point of amplification (6-14 cycles) ...
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...