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Citations for Roche :
1301 - 1350 of 6826 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 40 mM Tris-HCl pH 7.5, 20 mM MgCl2, and 4% Triton X-100 supplemented with 100 U/ml SUPERase-IN and 1X Roche protease inhibitor cocktail), and 24 ml nuclease free water on ice for 20 min with frequent mixing ...
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bioRxiv - Microbiology 2021Quote: ... 500 mM NaCl and 4 mM β-mercaptoethanol (β-ME)) in the presence of an anti-protease cocktail (Complete EDTA free, Roche) and 1 ul benzonase (MERK Millipore 70746) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 10,000 RPM for 30 minutes at 4°C and resuspended in 40mL lysis buffer (20mM HEPES, pH 7.4, 1M NaCl, 1mM EDTA, 1mM DTT, Roche protease inhibitor cocktail). Lysozyme was added at a final concentration of 1mg/ml and cells were incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Genomics 2022Quote: ... then incubated with primary antibody at 4°C overnight in Wash Buffer (10 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM spermidine, Roche Complete Protease Inhibitor Cocktail) with 2mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... 0.2 μL of each primer (100mM stock) (Table SI-4) and 5 μL of 2× KAPA SYBR Fast Universal qPCR kit (KAPA Biosystems, USA). Samples were cycled (40 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... was first pooled before being divided into 700-µL aliquots in cryovials containing 28 µL of a 25x concentrated protease inhibitor cocktail solution (4% v/v) (cOmplete Protease inhibitor cocktail tablets, Roche, Mannheim, Germany) according to the manufacturers’ instructions and stored at -80 °C (Figure 10 – step III).
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bioRxiv - Neuroscience 2022Quote: ... The cell viability following 4-AP treatment was assayed by MTT assay according to manufacturer’s protocols (Roche, cell proliferation kit I (MTT), # 11465007001) ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 1 h (at room temperature) before incubating overnight at 4 °C in anti-dig sheep Fab fragments conjugated to alkaline phosphatase (AP; Roche No. 11093274910) diluted 1:2500 in blocking solution ...
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bioRxiv - Pathology 2020Quote: ... The specific pairs of primers used for the real-time PCR are listed in Supplementary Table 4 (designed by Universal ProbeLibrary Assay Design Center tool from Roche, Indianapolis, IN). The real-time PCR was performed with FastStart Universal Probe Master mix ...
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bioRxiv - Genetics 2020Quote: ... The eluted protein was resolved on a 4-12% Bis-Tris gel (Novex, NP0322BOX) and ubiquitinated Cse4 was detected by Western blot using anti-HA antibody (Roche Inc., 12CA5). Levels of ubiquitinated Cse4 relative to the non-modified Cse4 in the input were quantified using software provided by the Syngene imaging system ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell pellets were lysed in 300 µL of lysis buffer (10 mL BugBuster® [Millipore 70584-4], ¼ tablet cOmplete™ Protease Inhibitor Cocktail EDTA-Free [Roche]) for 20 min at room temperature and centrifuged at 13,000 rpm at 4℃ for 30 min to clear all cell debris ...
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bioRxiv - Cell Biology 2022Quote: ... Tissue was transferred to a 50 mL conical tube and resuspended in DPBS/BSA containing collagenase B (2-4 mg/mL) (Roche, Mannheim, Germany) and DNase I (2,000 U/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4 °C in blocking solution supplemented with the alkaline phosphatase coupled anti-digoxigenin antibody (Roche, REF 11093274910) 1:3000 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative PCR was performed in 4 replicates in final volume of 10 µL using Light SYBR Green PCR Master Mix (Roche, Applied Science) on a LightCycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were pelleted and resuspended in 2× SDS-PAGE solvent (4% SDS, 20% glycerol, 0.1 M DTT, 0.125 M Tris-HCl pH 7.5 and Roche EDTA-free protease inhibitor cocktail) and boiled at 95°C for 5min ...
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bioRxiv - Bioengineering 2023Quote: ... 10-12 DRGs per donor were disintegrated into micro tissue units using 6 mL collagenase P (4 mg/mL, Roche, Mannheim, DE) in a 15 mL Falcon tube on an orbital shaker for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: Proteasome activity was measured by spectrofluorometry using the 7-amino-4-methylcoumarin (AMC)-labeled fluorogenic substrate succinate-LLVY-AMC (Roche Sigma-Aldrich) in cleared extracts of leaf 1 at different dpg (Üstün and Börnke ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Cell Biology 2021Quote: ... 1×proteinase inhibitors (Roche). Anti-cyclin D1 antibody (2 μg ...
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bioRxiv - Genomics 2020Quote: ... 1× protease inhibitor (Roche), 0.4 U/μl RNase inhibitor (RNaseOUT ...
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bioRxiv - Cancer Biology 2021Quote: ... 1× protease inhibitor (Roche), and 1 mM ATP and incubated for 1 h at 30 °C ...
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bioRxiv - Physiology 2020Quote: ... 1% Trypsin (210234, Roche), 1% collagenase D (11088866001 ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP (1:3000, Roche) and Tubulin (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... and 1× PhosSTOP (Roche). Cell lysate was centrifuged at 17,700 x g for 15min ...
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bioRxiv - Neuroscience 2020Quote: ... αHA (Roche, 1:500), IRDye 800 (Li-Cor 1:10000) ...
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bioRxiv - Neuroscience 2020Quote: ... and DNAse 1 (Roche, Catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µl Midazolam (Roche Pharma AG ...
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bioRxiv - Immunology 2022Quote: ... 1 mM dNTP (Roche), 2.5 mM DTT (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... GFP (Roche, 1/100) and pH3 (Abcam ...
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bioRxiv - Biophysics 2020Quote: ... 1 μg pyrophosphatase (Roche) and 10 μg purified T7 RNA polymerase (in house) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM ATP (Roche), and stated [NaCl] ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Blocking reagent (Roche) and 10% goat serum ...
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bioRxiv - Immunology 2021Quote: ... 1 mM phenylmethylsulfonylfluoride (Roche), and 1% octyl-β-D glucopyranoside (Sigma-Alrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 1%PIC (05892970001, Roche)) plus 0.25% RNase inhibitor for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × protease inhibitor (Roche)) ...
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bioRxiv - Biochemistry 2023Quote: 1 mM PefaBloc (Roche), 1mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies (1:2000, Roche). The ACTIN loading control was detected using anti-ACTIN C4 mouse antibody (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 × protease inhibitor (Roche), 20mM DTT] ...
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bioRxiv - Immunology 2023Quote: ... 1 mM dNTP (Roche), 2.5 mM DTT (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1× PhosSTOP (Roche). Heavy membrane fractions were pelleted by centrifugation at 14,000 g for 5 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 1× protease inhibitors (Roche) and Phosphostop (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... 1× cOmplete (Roche, 4693132001) protease inhibitor] and sonicated on a Covaris S220 Focused-ultrasonicator with the following parameters:15 cycles of 45 seconds ON (peak 170 ...
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bioRxiv - Cell Biology 2023Quote: ... 12CA5 (Roche, 1:2000), a-Flag ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin (Roche) and 1% insulin-Transferrin-Selenium (ITS ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM Pefabloc (Roche) and one tablet of complete protease inhibitor cocktail (Roche) ...