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Citations for Roche :
1251 - 1300 of 2081 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Western blot was done using standard procedures using anti-HA antibody (Roche 11867423001) and anti-GFP antibody (JL-8 ...
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bioRxiv - Synthetic Biology 2024Quote: Biotinylated anti-HA antibodies (50 μg/ml, High Affinity, 12158167001, Roche, Sigma-Aldrich) were mixed with streptavidin (S4762 ...
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bioRxiv - Microbiology 2024Quote: TssB-GFP fusions were identified using anti-GFP mouse monoclonal antibodies (1181446001; Roche) at a 1:5,000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... we applied peroxidase-conjugated anti-fluorescein antibody (catalog. no. 11426346910, Roche; RRID: AB_840257) on sections and performed TSA Plus biotin system (catalog ...
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bioRxiv - Microbiology 2024Quote: ... Western blotting was performed using a rat monoclonal antibody against HA (Roche, 3F10) or a control goat polyclonal antibody against GFP (Sicgen ...
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bioRxiv - Neuroscience 2024Quote: ... Probes were detected using an anti-digoxigenin antibody coupled to alkaline phosphatase (Roche) and NBT/BCIP (Roche ...
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bioRxiv - Genetics 2024Quote: ... Pds1 levels were monitored using rat anti-HA antibody (Cat. No. 11867423001, Roche) and peroxidase-conjugated rabbit anti-rat secondary antibody (Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP was detected with monoclonal anti-GFP antibody (cat.# 11814460001, Roche, RRID: AB_390913) and equal loading was assessed by staining with monoclonal anti α-tubulin antibody (cat ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was incubated with an anti-HA high-affinity antibody (Roche) coupled to protein A-sepharose (GE Healthcare) ...
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bioRxiv - Physiology 2024Quote: ... Primary antibodies used were: rat anti-HA (1:1000, Merck/Roche, #ROAHAHA, RRID:AB_2687407) and guinea-pig anti-Cv-d (1:2000 61 ...
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bioRxiv - Plant Biology 2024Quote: ... The blocked membrane was probed with global eEF2 antibody (3C2, 1:8,000, Roche) or diphthamide-unmodified eEF2 antibody (10G8 ...
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bioRxiv - Genomics 2024Quote: ... or with rat anti-HA antibodies conjugated with HRP (Roche, Sigma, cat# 12013819001). Images were taken on a Fusion FX imager (Vilber ...
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bioRxiv - Developmental Biology 2024Quote: ... before incubating the samples with anti-digoxygenin-alkaline phosphatase antibody (Roche, 1:4000) for 1h ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated with an anti-digoxygenin-alkaline phosphatase antibody (Roche, 1:5000) in blocking solution for 1h ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were then incubated with an Anti-DIG-AP antibody (Roche 11093274910) with a dilution of 1:5000 overnight at 4°C ...
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Developmental functions of rapgef1b in neural crest specification and presomitic mesoderm patterningbioRxiv - Developmental Biology 2024Quote: ... The embryos were then incubated with an Anti-DIG-AP antibody (Roche 11093274910) with a dilution of 1:5000 overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Primary (and conjugated) antibodies: rat anti-HA-peroxidase clone 3F10 high affinity (Roche), mouse anti-His clone 1B7G5 (Proteintech) ...
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bioRxiv - Cell Biology 2024Quote: ... or immunofluorescence studies the primary antibodies used were rat anti-HA (3F10, Roche), mouse anti-HA (12CA5 ...
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bioRxiv - Microbiology 2020Quote: ... The predicted structure was send to the FunFOLD2 server for the prediction of protein–ligand interactions (Roche et al., 2013). In addition ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed after 24 hours and protein was extracted with RIPA buffer with 1x protease inhibitors EDTA-free (Roche). Protein expression was measured through LICOR Western blotting as described above.
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bioRxiv - Cell Biology 2020Quote: ... The pellets were washed once with Buffer A and then further lysed with 250 μl modified RIPA buffer (1mM EDTA, 150mM NaCl, 0.1% Na-DOC, 1% NP-40, 50Mm Tris pH7.5 and Complete Roche protein inhibitors). 50U of benzonase (nuclease ...
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bioRxiv - Cell Biology 2020Quote: ... generation of total protein from MEF cells was contained by homogenization of cells in lysis buffer containing protease inhibitor (Complete, Roche). For determination of G/F-actin ratio fractionation was performed as described in Huang et al ...
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bioRxiv - Genomics 2021Quote: Reversal of crosslinks and protein degradation: The chromatin samples were incubated with 200 μl proteinase K digestion buffer containing 30 μg Proteinase K (Roche), 0.2 M NaCl and 30 mM Tris-HCl at 65°C for 12 hours.
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bioRxiv - Neuroscience 2021Quote: Protein samples were extracted from cells using ice-cold RIPA buffer with complete protease inhibitor mixture (Roche Applied Science, 04693124001). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Then cells were lysed in B-per protein extraction reagent 4 ml/ gram bacterial pellet in the presence of cOMPLETE mini protease inhibitor cocktail (Roche) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysis buffer was supplemented with protease and protein phosphatase inhibitor tablets (04-693-159-001 and 04-906-845-001 respectively, Roche). Protein content was determined with the BCA Protein Assay kit (23225 ...
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bioRxiv - Developmental Biology 2022Quote: ... Collected tissues were ground in ice-cold protein lysis buffer (Beyotime, Shanghai, China) containing protease inhibitors cocktail (Roche, Basel, Switzerland) for protein extraction ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Neuroscience 2021Quote: ... the lysates were centrifuged (10 min, 20000 g, 4 °C) and the resulting supernatants were added to 25 µl Protein G Agarose (Roche), rotating for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified by centrifugation and incubated with α-Pk clone SV5-Pk1 (AbSerotec) orα-HA clone 12CA5 (Roche) antibodies for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Cancer Biology 2020Quote: Protein extracts were prepared from drug-treated cells by lysis on ice for 20 minutes in M-PER Mammalian Protein Extraction Reagent (ThermoScientific) supplemented with protease and phosphatase inhibitors (Roche). Lysates were centrifuged at 16,000 RPM for 20 minutes at 4°C and the supernatants were assessed for concentration via a Bradford assay ...
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bioRxiv - Microbiology 2020Quote: Further protein extraction steps for each of 10 samples were carried out in solutions supplemented with cOmplete protease inhibitors (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were obtained from tissues or cells using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Roche, USA). Western blotting was conducted as described previously (Yao et al. ...
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bioRxiv - Neuroscience 2022Quote: ... At DIV 17 cells were washed with ice-cold PBS and immediately extracted with ice-cold T-PER protein extraction buffer (Pierce) supplemented with inhibitors for proteases and phosphatases (Roche). The lysates were centrifuged at 15000 g for 15 minutes at 4°C and the supernatants were used for further analysis.
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bioRxiv - Neuroscience 2022Quote: ... Lysates were diluted to a final total soluble protein concentration of 1 mg/ml in TBS containing cOmplete mini protease inhibitor tablet (Roche). For AD ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were isolated from cell cultures in mid-exponential phase in Hepes 50mM supplemented with EDTA-free protein inhibitor cocktail (Roche). Cell walls were broken by three 30-s cycles at 10000 rpm in the presence of micro glass beads (200-400 µm diameter Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Cell Biology 2022Quote: ... incubation at 4 °C rotating for 3 h, followed by incubation (4 °C, rotating) with 30 μl of pre-washed Protein A agarose bead slurry (#11134515001, Roche) for an additional hour ...
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bioRxiv - Microbiology 2021Quote: ... GTP binding Irgb6 crystals diffracting to 1.5 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GTP (Roche) and a reservoir solution consisting of 0.1 M Sodium Citrate buffer pH 5.4 (Wako) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 200 μl of Laemmli sample buffer with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany) for 15 min at RT.
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bioRxiv - Microbiology 2020Quote: Western blot analysis of infected and uninfected erythrocytic material performed on protein extracts prepared by resuspending cells in M-PER mammalian protein extraction reagent (Pierce) supplemented with 1x protease inhibitory cocktail (EDTA free) (Roche), 300 mM benzamidine ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein extracted using RIPA buffer with protease inhibitor cocktail mix (1 Complete MINI EDTA-free protease inhibitor tablet (Roche), 25 μg/mL calpain inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Bioengineering 2022Quote: Ileal and colonic proteins were obtained by tissue homogenization in NP-40 Buffer with 1x cOmplete Mini Protease Inhibitor Cocktail (Roche). Protein concentrations were determined using Bradford Assay ...
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bioRxiv - Microbiology 2022Quote: ... GTP-binding Irgb6-T95D crystals diffracting to 1.68 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution containing 2 mM GTP (Roche) and a 0.5 μl of reservoir solution consisting of 0.2 M Sodium sulfate (Molecular Dimensions ...
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bioRxiv - Plant Biology 2022Quote: Total protein extracts were prepared by re-suspending 100 mg of ground plant material in 100 μl of protein lysis buffer (50 mM Tris pH 8.0; 2% SDS; 10 mM EDTA; protease inhibitors (Roche (04693159001) cOmplete™ ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were incubated with Protein A/G-Agarose beads (Cat# 11 134 515 001/ 11 243 233 001, Roche), and either ARP3 or normal Rabbit IgG antibody for 2 h at 4°C with rotating ...