Labshake search
Citations for Roche :
1251 - 1300 of 6565 citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... 100 mM Tris-HCl pH 8.0, 5% glycerol, 1 mM DTT, 0.1% CHAPS, 1 μg/mL avidin, cOmplete, EDTA-free protease inhibitors [Roche]), rotated at 4°C for 30 min ...
-
bioRxiv - Developmental Biology 2019Quote: ... and stained with standard Nitro Blue Tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) (Roche) ISH protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclei isolated from approximately 2.5x107 cells were resuspended in one ml of extraction buffer (50 mM TrisHCl pH 7.5, 150 mM NaCl, 10% glycerol, 5 mM DTT, 0.25%NP-40, 1x Roche Complete protease inhibitors ...
-
bioRxiv - Developmental Biology 2020Quote: ... nuclei were harvested using cell lysis buffer (5 mM PIPES, pH 8.0, 85 mM KCL, 0.5% NP40, and Roche cOmplete ...
-
bioRxiv - Developmental Biology 2020Quote: ... with some dams bearing 13.5 dpc embryos also intraperitoneally injected with 5-Bromodeoxyuridine (BrdU, Roche, 40 mg/kg) to track cell division ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 2 μl diluted cDNA (5 ng total RNA equivalents) were analyzed on the LightCycler 480 instrument (Roche) using two replicates ...
-
bioRxiv - Immunology 2020Quote: ... washed twice with PBS and lysed in 5 ml lysis buffer (50 mM Tris-HCL, ph7,4; 500 mM NaCl, 0,2% SDS; 1x protease inhibitors (Roche), 20 mM DTT ...
-
bioRxiv - Molecular Biology 2022Quote: ... The isolated nuclei were warmed at 37oC for 5 min and treated with micrococcal nuclease (MNase) (Roche 10107921001) and 2 mM CaCl2 (RICCA R1760000) ...
-
bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were lysed with 5mL cold lysis buffer (10 mM Tris-HCl, pH 7.5; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; 1x complete protease inhibitor; 11836145001 Roche) for 10 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 400 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, 5% glycerol, 1x protease/phosphatase inhibitor cocktails, Roche). Nuclear lysates were diluted with 2 volumes of dilution buffer (20 mM HEPES pH7.9 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 500 µL cold cell lysis buffer (5 mM PIPES pH8.0, 85 mM KCl, 0.5% NP-40, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
-
bioRxiv - Cancer Biology 2024Quote: ... pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen], protease inhibitor cocktail [Complete; Roche] ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and lysed for 20 min on ice in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40, supplemented with protease inhibitors [Roche]). Centrifugation at 13,000×g for 10 min at 4°C was then used to remove cell debris ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP, 20% glycerol, 5 mM imidazole, 1.4 μg/mL leupeptin, 1.0 μg/mL pepstatin A and two Roche EDTA-free protease tablet per 5 g cell pellet) ...
-
bioRxiv - Molecular Biology 2023Quote: ... DPCs were resolved on 5-18% SDS-PAGE gradient gels and transferred to a PVDF membranes (Roche, 03010040001). Membranes were blocked with 5% low-fat milk in TBST buffer (10mM TrisHCl (pH 7.5) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.2 g of powder was dissolved in 1.2 ml of microsome buffer (50 mM MOPS, 0.5 M sorbitol, 5 mM EGTA, complete protease inhibitor (Roche) at pH 7.6) ...
-
bioRxiv - Molecular Biology 2023Quote: ... centrifuged and filtered expression medium was loaded onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... Colorimetric signals were generated using a development solution containing 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.5 µl gene specific primer mix (5 µl each) and 4.5 µl FastStart Essential cDNA Green Master (Roche) were amplified using 45 cycles of 25 s at 95 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 mM N-ethylmaleimide and Complete protease inhibitor cocktail (Roche, one tablet/10 ml of buffer). HA-tagged ubiquitin was purified using Pierce anti-HA magnetic beads (clone 2–2.2.14) ...
-
bioRxiv - Bioengineering 2023Quote: ... 3 % NaCl (5 M, v/v), 0.08 % CaCl2 (2.5 M, v/v) and cOmpleteTM ULTRA Protease Inhibitor Cocktail tablets (Roche) in H2O ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM MgCl2, 0.25% IGEPAL CA-630, 1 mM DTT, cOmplete protease inhibitor [Roche]) and mixed with 200 µL of 3 µg µL−1 nuclear extract and 500 µL PBB+ buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 0.25% IGEPAL CA-630, 1 mM DTT, 5 mM MgCl2, cOmplete protease inhibitor by Roche). Per replicate and construct ...
-
bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 7.5, 0.5% IGEPAL CA-630, 5 mM MgCl2, 1 mM DTT, 1x protease inhibitor EDTA free [Roche]) and mixed with 50 µL PBB-buffer-equilibrated MyOne Streptavidin C1 Dynabeads (Thermo Scientific) ...
-
bioRxiv - Biophysics 2022Quote: ... 200 mM NaCl, 5 mM Beta glycerophosphate, 0.1 mM sodium orthovanadate, 2 mM TCEP, 0.4% NP40, 1X Roche EDTA free mini complete protease inhibitor) ...
-
bioRxiv - Genetics 2023Quote: Quantitative RT-PCR was performed in a final volume of 5 μl with SYBR Green I Master (Roche) and analyzed with a Lightcycler 480 (Roche ...
-
bioRxiv - Molecular Biology 2023Quote: ... Beads were washed three times with 200 µL of washing buffer (50 mM Tris-HCl at pH 7.5, 150 mM KCl, 5 mM MgCl2, 0.05% NP-40, EDTA-free cOmplete mini protease inhibitor [Roche, 11836153001] ...
-
bioRxiv - Immunology 2023Quote: ... HEK293T cells were lysed in LUMIER lysis buffer (50 mM Hepes-KOH pH 7.9, 150 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 5% glycerol and Roche cOmplete™ Mini protease Inhibitor Cocktail ...
-
bioRxiv - Cell Biology 2023Quote: ... 80 μL DNase I (5 mg/ml; DNase I – grade II from bovine pancreas from Roche (Basel, Switzerland)) ...
-
bioRxiv - Microbiology 2023Quote: ... after which they were resuspended in 1.0 ml of ice-cold lysis buffer (10 mM Tris-HCl, pH 7.7; 10 mM NaCl; 5 mM MgCl2; 0.1 mM EGTA; protease inhibitors [Roche]) and incubated on ice for 10 min before nuclei were pelleted (400 × g ...
-
bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
-
bioRxiv - Molecular Biology 2023Quote: Genomic DNA extracted from the parasites at passages 7 and 20 were digested with 5 U Rsa1 (Roche) at 37 °C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... pH 7.9; 5 mM MgCl2; 0.2% Triton X-100; 20% glycerol; 300 mM NaCl and freshly added ROCHE cOmplete protease inhibitor cocktail at 0.01 tablet/mL) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 µl cDNA and 5 µl of LightCycler 480 SYBR Green I Master mix (Roche Diagnostics GmbH, Germany). Three technical replicates of each sample were included ...
-
bioRxiv - Physiology 2024Quote: ... 5 mM EDTA, 2% Triton-X-100, 0.2 mM HDSF, pH 7.4, and protease inhibitor cocktail from Roche). Following sonication on ice ...
-
bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
-
bioRxiv - Developmental Biology 2024Quote: ... The binding reactions were separated on native 5% acrylamide gels and transferred onto positively charged nylon membranes (Roche) with a Perfect Blue Semi-dry electro blotter (60 minutes at 120volts ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1mM MgCl2, 0.1% NP-40, 5 mM DTT, 0.5 mM PMSF, 1× EDTA-free protease inhibitor cocktail [Roche] ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 0.5 % SDS, 1#x00D7; protease inhibitor cocktail from Roche). The resulting nuclei were spun down ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 mL of Farnham lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40, EDTA-free Roche protease inhibitor tablets ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in lysis buffer (50 mM HEPES, pH7.5, 100 mM KCl, 5% glycerol, 0.3% Triton X-100, 1X Roche EDTA-free Complete Protease Inhibitor cocktail ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Cells in 6-well plates were lysed with RIPA lysis buffer containing 1mM PMSF and 1% phosphatase inhibitor cocktail (PhosSTOP, Roche group, Swiss). The supernatant was collected after centrifuging at 14,000 rpm for 15 min at 4℃ ...
-
bioRxiv - Microbiology 2021Quote: ... A single sample (one mouse) was assessed on each array plate on a Roche Light Cycler 480 II real time PCR thermal cycler (Roche, Basel, Switzerland). All data were analyzed with Qiagen’s Gene Globe online analysis application ...
-
bioRxiv - Molecular Biology 2022Quote: ... Junction qPCRs were set up by dispensing crude gDNA and premixed junction primer pairs into a LightCycler® 1536 Multiwell Plate (Roche 05358639001) containing 1x LightCycler® 1536 DNA Green mix (Roche 05573092001) ...
-
bioRxiv - Microbiology 2023Quote: ... HEK293T/17 cells were seeded onto a 6-well plate and then transfected 24 h later using X-tremeGENE HP (Roche, Basel, Switzerland). After transfection (24 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... The seeding of target cells was performed in 100 μl of medium per well to E-Plates 96 (Roche, Grenzach-Wyhlen, Germany), and appropriate cell densities were determined through titration experiments ...
-
bioRxiv - Immunology 2023Quote: ... the dorsal and ventral layers of the ear were split mechanically and placed dermis side down in a 24 wells plate with 500 μl/well of RPMI with 250 mg/mL of Libarase (Roche, Cat #05401054001) and 10 mg/mL of DNase I (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... were sequenced in the forward direction on 1/8 plate of a 454 Life Sciences Genome Sequencer FLX machine (Roche, Branford, CT) using the Macrogen facilities (South Korea).