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Citations for Roche :
1251 - 1300 of 7613 citations for 8 CHLORO 2 CHLOROMETHYL 6 TRIFLUOROMETHYL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: pLKO.1 lentiviral construct along with packaging vector ΔVPR and VSVG plasmids were transfected using Fugene-6 (Roche) or PEI (POLYSCIENCES 23966-2 ...
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bioRxiv - Developmental Biology 2019Quote: ... third-instar larvae were dissected in PBS and incubated with 5-bromo-2’-deoxy-uridine (10 μM, Roche) for 45 min ...
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bioRxiv - Genomics 2020Quote: ... 2 μL of DNA were used per reaction with the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of 2× SYBR Green Real-time PCR Master Mix (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction (20 μL) contained 10 μL of SYBR Green Real-time PCR Master Mix (2×) (Roche), 0.25 μM primers ...
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bioRxiv - Neuroscience 2019Quote: ... for 12 concentrations in sample buffer (0.1% BSA/0.05% Tween/PBS, 2 tablets of cOmplete Protease Inhibitor Cocktail (Roche Diagnostics Corporation ...
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bioRxiv - Genomics 2019Quote: ... Cell nuclei pellet was re-suspended in 2 mL FACS buffer (0.15 mM spermine, 0.5 mM spermidine, Complete protease inhibitor (Roche) and 20mM Na-butyrate (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... rinsed in TBS/0.1% Tween-20 and incubated overnight in blocking buffer (TBS with 2% goat serum, 0.1% blocking reagent (Roche), 0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified carcinoma organoids were dissociated into single cell suspension in 0.05% trypsin with 0.1% EDTA supplemented with 2 U/ml of DNase I (Roche) for 2-3 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Pancreas was minced into 2- to 3-mm pieces and digested with 0.2 mg/ml collagenase P (Roche) at 37 °C for 10-12 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 40 mM HEPES, pH 7.4, 2 mM EDTA, 10 mM pyrophosphate, 10 mM glycerophosphate, 0.3% CHAPS, protease inhibitors from Roche) as indicated.
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bioRxiv - Molecular Biology 2020Quote: Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Roche, Mannheim, Germany), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Patient cfDNA was isolated from 2 mL of plasma using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 genomic RNA was isolated from cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNAs diluted 2 times were examined by qPCR with the KAPA SYBR Fast qPCR Kit (Kapa Biosystems, K4602) using specific primers for β-actin ...
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bioRxiv - Cancer Biology 2021Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (11465007001, Roche Diagnostics, Mannheim, Germany) was performed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... before being detached with 40 μL of ice-cold native lysis buffer (80 mM PIPES pH 6.9, 2 mM MgCl2, 4 mM EGTA, 0.2% saponin, 5x cOmplete protease inhibitor cocktail Roche). The lysate was collected in 1.5 mL tube and incubated on ice for 10 min ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Afterwards samples were incubated for 2 h in the In Situ Cell Death Detection Kit (Roche, Basel, Switzerland) enzyme labeling solution at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.4 mM KCl,10 mM CaCl2, 2.4 mM NaHcO3, 2 mM HEPES, pH7.0 and a protease inhibitor tablet [Roche]). The cell suspension was incubated at 4 °C for 15 mins with rigorous end-over-end rotation ...
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bioRxiv - Immunology 2019Quote: ... NK92 cells were maintained in Myelocult medium supplemented with penicillin/streptomycin and 100 U/ml IL-2 (Roche). CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested for 30 minutes at 37 °C in DMEM + 2 mg/mL Collagenase A (Roche, Basel, Switzerland) + DNase (Roche ...
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bioRxiv - Immunology 2020Quote: ... patients with at least one nasopharyngeal swab positive for SARS-CoV-2 as determined by qRT-PCR (Roche LightCycler480 ...
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bioRxiv - Plant Biology 2019Quote: ... water was replaced with 100 μl of a 2 μg/ml horseradish peroxidase (type II; Roche, Rotkreuz, Switzerland) and 5 μM L-012 (WAKO Chemicals ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in Lysis buffer (50 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT, 0.3 % TritonX-100, Roche Complete protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2021Quote: Lysis buffer 2x (200 mM Tris, 300 mM NaCl, 2% triton, pH 7.4) and Complete proteinase inhibitor (Roche) were added to homogenized organs for 30 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris, pH 7.2, 125 mM NaCl, 2 mM DTT, 0.1% Triton X-100, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2019Quote: ... Drug cytotoxicity was evaluated by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (Cell Proliferation Kit I, Roche) with a NanoQuant microplate reader (Tecan Trading AG ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated in 2 ml HBSS solution containing 0.21 mg/ml Liberase TL (Roche; cat: 5401020001) or DH (Roche ...
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bioRxiv - Immunology 2021Quote: ... The Roche Elecsys anti-SARS-CoV-2 assay was performed on Roche Cobas e411 (Roche Diagnostics, Indianapolis, IN). The Elecsys antinSARSnCoV-2 assay uses a recombinant protein representing the N antigen for the determination of antibodies against SARSnCoVn2 ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... extremities and organs were removed from E13.5 embryos and the remainings were enzymatically digested with Blendzyme 2 (0,5 mg/ml; Roche Applied Sciences ...
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bioRxiv - Microbiology 2021Quote: The cytotoxicity assays were performed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Roche, ref:11465007001) protocols according to the previous study [47] with few modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 μl diluted cDNA (5 ng total RNA equivalents) were analyzed on the LightCycler 480 instrument (Roche) using two replicates ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of saliva sample was mixed with 2 µL of 10 U/µL α2-3,6,8-Neuraminidase (Sialidase) from Arthrobacter ureafaciens (Roche) and reacted at 37°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Pellet was resuspended in 2 mL of homogenization buffer (250mM sucrose, 10mM HEPES, 1mM EDTA, protease inhibitors (Roche), 1mM PMSF ...
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bioRxiv - Developmental Biology 2020Quote: ... and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Immunology 2021Quote: ... pH 7.2) with 2 mM PMSF and EDTA-free Complete Protease Inhibitors Cocktail tablets (Roche Diagnostics, Mannheim, Germany), and its supernatant was transferred onto 30-60% sucrose gradient ...
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bioRxiv - Genomics 2020Quote: ... Specimens in May were tested using the Cobas® SARS-CoV-2 RT-PCR assay (Roche Diagnostics, USA). Respiratory samples archived at the Clinical Laboratory ...
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bioRxiv - Cell Biology 2022Quote: ... Inguinal stromal-vascular fractions (SVF) were isolated from 2-week-old C57BL/6J mice with collagenase D (Roche) digestion and differentiated as described previously34 ...
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bioRxiv - Microbiology 2022Quote: ... was used to detect SARS-CoV-2 mRNAs and visualized by DIG luminescent detection kit (Roche, Indianapolis, IN), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The nuclear lysates were diluted 2 fold with IP buffer (20mM HEPES, pH 7.9, 0.2M NaCl, complete protease inhibitors (Roche) and then flag-beads (Anti-Flag Magnetic Beads ...
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bioRxiv - Genetics 2022Quote: ... 50 mM Hepes pH 7.6, 2 mM DTT, 0.1 mM PMSF and supplemented with complete protease inhibitor tablets, Roche) were added dropwise and on a vortex with constant low speed to ensure immediate mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were collected and washed with ice-cold 1X PBS before being resuspended in Sonication Buffer (20 mM Tris, pH 8.0, 2 mM EDTA, 0.5 mM EGTA, 1X Complete Mini EDTA-free Protease Inhibitor Cocktail [Roche 11836170001] ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... 0.1% NP-40) containing 2 μM MG-132 and cOmpleteTM mini EDTA-free protease inhibitor cocktail (Roche, #04693159001) for 30 minutes at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The oocytes were homogenized in 2 ml Lysis buffer (Tris-HCl pH 8.0 50 mM, ½ tablet PhosSTOP (Roche), 1x cOmpleteTM EDTA-free proteinase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Peritoneal cavities were lavaged by injection of 2 mL of sterile PBS containing 1X protease inhibitors (cOmplete, Roche) or stable isotope labeling with amino acids in cell culture (SILAC ...
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bioRxiv - Genomics 2024Quote: ... Cells were re-suspended in digestion buffer (50 mM Tris-HCl pH7.6, 2 mM CaCl2, 0.2% NP40) containing protease inhibitors (complete EDTA-free, Roche) and warm-up to 37°C for 2min ...
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bioRxiv - Immunology 2024Quote: ... System performance was assessed by an Fc/2 standard obtained from a commercial mAb preparation (Herceptin; Roche Diagnostics).
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bioRxiv - Biochemistry 2024Quote: Cell metabolic viability was measured by the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Roche). After treatments ...