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Citations for Roche :
1251 - 1300 of 7863 citations for 6 Chloro 1 phenylsulfonyl 1H pyrrolo 2 3 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with 1 µg of shRNA/cDNA and 1 µg of viral packaging plasmids (250 ng pMD2.G and 750 ng psPAX2) using 6 µl of Xtreme Gene 9 transfection reagent (Roche). After 24 hours of transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of 106 cells in a 6-well plate was performed using 5 μl of transfection agent (X-tremeGENE HP Reagent, Roche), 10 μL of bacmid and 85 μL of SF900II-SFM medium (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... Then the different pellets were resuspended for 30 min in denaturing buffer (20 mM Tris, 500 mM NaCl, 6 M Guanidine, 20 mM Imidazole and protease inhibitor cocktail (Roche), pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... D-glucose was measured spectrophotometrically3 by reading the increase in NADPH(H+) absorbance at 340 nm produced in two coupled reactions catalyzed by hexokinase and glucose-6-phosphate dehydrogenase (G6PD) (Roche diagnostics Corporation ...
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bioRxiv - Molecular Biology 2023Quote: For nuclear cell lysis 5×10^6 cells per condition were resuspended in 1mL of Lysis Buffer + protease inhibitor (cOmplete, Roche) (10mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded at 400,000 cells per well in 6-well plates and transfected the day after with indicated cDNA constructs with FuGENE6 (Roche). Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were seeded 72 hours prior to the experiment at 400,000 cells per well in 6-well plates and transfected the next day with wt or mutated myc-ALPK1 constructs with FuGENE6 (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Proteins were harvested from ∼30 mg of tissue (in vivo) or two 6-well plates (in vitro) and homogenised in Ripa buffer with complete mini-proteinase inhibitors (Roche). Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed 6 times with MABT and were then incubated with anti-DIG AP-conjugated Fab fragment (Roche, 11093274910), Alexa-Fluor 488 goat anti-Mouse IgG1 antibody (ThermoFisher A-21121 ...
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bioRxiv - Biochemistry 2023Quote: ... values of corresponding cDNAs with the QuantStudio 6 Pro system (Applied Biosciences, A43180) and FastStart Essential DNA Green Master (Roche) at a final concentration of 1X ...
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bioRxiv - Cancer Biology 2023Quote: ... pUMVC for retrovirus) and 250 ng envelope vector (pCMV-VSV-G) are mixed with 20 µL Fugene 6 (Roche, USA) diluted in OptiMEM ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were amplified by ligation-mediated PCR for 6 cycles using a KAPA HiFi HotStart high-fidelity DNA polymerase (Roche) and purified using AMPure XP beads.
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bioRxiv - Neuroscience 2024Quote: ... and Str tissue from old (N =6 wt, N =8 tgNM) mice were homogenized in RIPA buffer supplemented with protease inhibitors (Roche) and cell extracts clarified by centrifugation at 13000g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... TNF and IL-6 were measured by flow cytometry and plasma glucose was measured with Accu-Chek Aviva Testrips (Roche).
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bioRxiv - Molecular Biology 2024Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche), and the cells were fixed 5–6 days later ...
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bioRxiv - Genomics 2022Quote: ... Samples were ground to powder in liquid nitrogen and then dissolved in 2 mL lysis buffer (8 M urea, 2% SDS, 1x Protease Inhibitor Cocktail (Roche Ltd. Basel, Switzerland), followed by sonication on ice for 30 min and centrifugation at 13 000 rpm for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 3 % SDS and 5 mM EDTA) supplemented with 1x complete protease inhibitor cocktail (Roche, Switzerland) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Neuroscience 2020Quote: ... which were stained with 5-bromo-4-cloro-3-indlyl phosphate/nitro blue tetrazolium (Roche) chromogenic substrates.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then pelleted and washed 3 times with PBS containing protease inhibitor (Roche, 11873580001). The pellets were snap-frozen and stored at -80°C for later use ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed at 3 days in ice-cold RIPA buffer containing protease inhibitors (Roche) and protein concentration was determined by BCA Assay (Gibco BRL ...
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bioRxiv - Genomics 2019Quote: ... beads were washed 3×1mL with bead wash buffer (1x PBS, 5mg/mL BSA, Roche complete EDTA-free protease inhibitor ...
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bioRxiv - Molecular Biology 2022Quote: To determine the repM transcription start site a 2nd Generation 5’/3’ RACE Kit (Roche) was used according to the manufacturer’s instructions with some modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer2: 5’-CAAGCAGAAGACGGCATACGA*G-3’) and 15µl 2x Kapa HiFi HotStart Ready Mix (Kapa Biosystems), amplification was performed for 45 s at 98°C ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were then prepared from 3 ng DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Physiology 2023Quote: ... 3 µL of Light Cycler 480 SYBR® Green I Master mix (Roche Diagnostics, Switzerland), 0.24 µL of each primer (10X ...
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bioRxiv - Genomics 2023Quote: ... For 3-color detection the following antibody dilutions were made: anti-digoxigenin (Roche, cat. 11333089001) 1:10 ...
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bioRxiv - Genomics 2021Quote: ... and then resuspended in Antibody buffer (10 mM HEPES pH 150 mM NaCl, 2 mM spermidine, 2 mM EDTA, 0.1% BSA, and Roche complete EDTA-free protease inhibitor) with primary anti-Pol2S5p antibody (Cell Signaling Technology cat ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2 and EDTA-free protease inhibitor cocktail (Roche)) and lysed in an ice-cold sonicating water bath for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 25 μl of 2× HIFI HotStart Ready Mix (Kapa Biosystems), and 0.2 M SMART PCR Primer and polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... PMSF with 2 cOmplete protease inhibitor cocktail tablets (Roche 04693116001) per 50 mL of buffer) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg/ml RNase A and protease inhibitor mixture (Roche). Proteins were bound to GST resin (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg/ml Rnase A and protease inhibitor tablets (Roche)) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP and a cocktail of protease inhibitors (Roche) using a syringe fitted with a 26G ½ needle ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 2% FCS and 1mg/mL dispase II (Roche) overnight at 4°C ...
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bioRxiv - Systems Biology 2019Quote: ... 2% SDS and protease inhibitors (complete mini., EDTA-free; Roche). Cells were lysed by sonication ...
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bioRxiv - Genomics 2021Quote: ... 0.0075% DIG and 2 U/ul Protector RNase inhibitor (Roche)) for 1 min on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... The embryos were blocked with 2% Blocking Reagent (Roche, Mannheim) for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 ng/mL fibroblast growth factor 2 (FGF2, Roche). Oligodendrocyte precursor cells were removed by brusquely shaking the culture flasks several times when changing the medium after 2 or 3 days ...
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bioRxiv - Immunology 2019Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The A20 cells were grown in RPMI-1640 (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... and a phosphatase inhibitor cocktail (2×PhosSTOP from Roche Diagnostics). Proteins were quantified using the Bio-Rad DC Protein assay (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2% SDS) in the presence of cOmplete and PhosSTOP (Roche). Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% 2-mercaptoethanol) in the presence of protease inhibitors (Roche) and phosphatase inhibitors (1 mM sodium orthovanadate ...
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bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
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bioRxiv - Cell Biology 2021Quote: ... PMSF (2 mm) and Protease Inhibitor Tablet (Roche, Basel, Switzerland), RNase A (1 mg·mL−1 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM EDTA pH 8.0) containing Protease Inhibitor Cocktail (Roche). Nuclei were lysed using a Misonix XL-2000 Probe Sonicator with 5-10 second ON / 20 second OFF intervals until the lysate was clear (2 rounds for P0 ...
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bioRxiv - Immunology 2022Quote: ... 2 mM EDTA and 10% glycerol with protease inhibitors (Roche) and phosphatase inhibitors (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... The lungs were inflated with dispase (2 U/mL; Roche) in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... and 60 U/mL human IL-2 (Proleukin, Roche Diagnostics). The F9 teratocarcinoma cells were grown in DMEM (Gibco ...