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Citations for Roche :
1201 - 1250 of 8498 citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, 5% glycerol, 1x protease/phosphatase inhibitor cocktails, Roche). Nuclear lysates were diluted with 2 volumes of dilution buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM MgCl2, 0.25% IGEPAL CA-630, 1 mM DTT, cOmplete protease inhibitor [Roche]) and mixed with 200 µL of 3 µg µL−1 nuclear extract and 500 µL PBB+ buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 0.25% IGEPAL CA-630, 1 mM DTT, 5 mM MgCl2, cOmplete protease inhibitor by Roche). Per replicate and construct ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 7.5, 0.5% IGEPAL CA-630, 5 mM MgCl2, 1 mM DTT, 1x protease inhibitor EDTA free [Roche]) and mixed with 50 µL PBB-buffer-equilibrated MyOne Streptavidin C1 Dynabeads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... 1mM MgCl2, 0.1% NP-40, 5 mM DTT, 0.5 mM PMSF, 1× EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 0.5 % SDS, 1#x00D7; protease inhibitor cocktail from Roche). The resulting nuclei were spun down ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Genetics 2024Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM MgCl2, 1 mM EGTA, 0.1% [v/v] NP-40, 1 mM DTT, 5% [v/v] glycerol and Roche Complete Protease Inhibitors) and homogenized using a high-performance disperser (Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2% NP-40, 10% glycerol, 1 mM EDTA, 1 mM PMSF, 20 µM MG132, 5 mM DTT and Roche protease inhibitor #5892953001), and incubated for 1 h on a rotating wheel ...
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bioRxiv - Plant Biology 2021Quote: Frozen inflorescence tissue was ground with liquid nitrogen and resuspended in lysis buffer (50 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 1% IGEPAL, 0.5 mM DTT, 1 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG; or anti-GFP, 1:1000, Roche #11814460001 in 3% BSA/TBS-T) at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were washed three times for 5 minutes with PBSTx and incubated in a blocking solution (5% sterile horse serum + 0.5% Roche Western Blocking Reagent in PBSTx) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... the same procedure was used using different lysis (50 mM HEPES pH 7.4, 100 mM NaCl, 10% glycerol, 1 mM DTT, 1 mM ATP, 2 mM PMSF, 1 Roche tablet per 50 mL) and storage (50 mM Tris pH 7.4 ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were lysed in 50 µl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1× Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Plant Biology 2024Quote: ... were finely chopped into pieces in cold nuclei extraction buffer (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 3% triton X-100, 1% Roche protease inhibitor cocktail tablet). The resulting mixture was filtered through a 40 μm cell sieve into a collection tube and centrifuged at 1,000 g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed with the same cold nuclei extraction buffer (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 3% triton X-100, 1% Roche protease inhibitor cocktail tablet) and then resuspended in nuclei purification buffer (20 mM MOPS pH 7.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration) and then dialyzed into 10 mM Tris pH 7.5 with 2 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of pefabloc (Roche, 5 mM final concentration), then dialyzed into 10 mM tris(hydroxymethyl)aminomethane (Tris ...
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bioRxiv - Immunology 2021Quote: ... containing 20 uL/mL Liberase TL (5 mg/ml, Roche) and 50 U/ml DNAse I (Roche ...
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bioRxiv - Genetics 2020Quote: ... 5 µL of 2x Kapa library amplification mix (Roche, KK2702), 0.2 µL forward primer (10 µM ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U/mL rabbit pyruvate kinase (Roche Diagnostics, Cat# 10128155001), 8 U/mL lactate dehydrogenase (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM BME) including cOmplete EDTA-free protease inhibitor (Roche) and subjected to lysis by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg DNase I (from Bovine Pancreas grade II, Roche) was added to 50 mL of lysate ...
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bioRxiv - Microbiology 2020Quote: ... 5% Na deoxycholate) supplemented with protease/phosphatase inhibitor cocktail (Roche) and clarified from the nuclei by centrifugation at 13,000×g for 10 min at 4°C for quantitative western blot analysis (see below) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol and mini-complete EDTA-free protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM β-mercaptoethanol supplemented with protease inhibitor cocktail (Roche). Cell lysis was accomplished by sonication ...
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bioRxiv - Cell Biology 2021Quote: ... 5% glycerol and protease inhibitors (Complete Protease Inhibitor Cocktail, Roche). The lysate was centrifuged at 160,000 x g for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... phosSTOP phosphatase inhibitor mixture (0.5 tablet for 5 mL, Roche) and 1% n-octylglucoside in 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Cell Biology 2022Quote: ... 5% glycerol) supplemented with EDTA-free cOmplete protease inhibitor (Roche). Samples were incubated on ice with intermittent agitation by pipetting for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol] supplemented with EDTA-free protease inhibitor cocktail (Roche), sonicated ...
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bioRxiv - Molecular Biology 2020Quote: ... together with 5 µl of a 1kB DNA Ladder (Roche). The electrophoresis was performed at 90 V constant.
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bioRxiv - Microbiology 2021Quote: ... 5% glycerol) with cOmplete EDTA-free protease inhibitor cocktail (Roche) and disrupted by sonication for 3 min (10 s ON ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and midazolam (5 mg/ml; Hoffmann - La Roche, Hvidovre, Denmark), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... It was subsequently incubated with 5 μg GFP antibody (Roche) or normal mouse IgG (santa cruz ...
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bioRxiv - Evolutionary Biology 2021Quote: ... blocked with 5% blocking solution (Roche, 11 921 637 001) in PBT for at least two hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Midazolam (5 mg/kg; Dormicum, Hoffmann-La Roche, Basel, Switzerland) and Medetomidine (0.5 mg/kg ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 5 µg/ml human plasma fibronectin (Roche, 11080938001) in growth medium overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 mM EDTA) containing Protease Inhibitor Cocktail (Roche, 11836170001). The DC Protein Assay (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Revealing was performed by 5 min incubation with LumiLightPlus (Roche) substrate on a Chemidoc MP Imaging system (BioRad) ...