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Citations for Roche :
1201 - 1250 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the KAPA mRNA Capture Kit (Roche, Basel, Switzerland), and the MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Developmental Biology 2024Quote: - LightCycler - RNA Control Kit (Roche Applied Science, Germany)
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bioRxiv - Cell Biology 2020Quote: Mammalian cell lysates were prepared in radioimmuno-precipitation assay (RIPA) buffer supplemented with 1 tablet of EDTA-free protease inhibitor (Roche, Cat No. C762Q77) per 25ml buffer ...
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bioRxiv - Microbiology 2020Quote: Deidentified remnant patient samples that underwent routine clinical testing with the cobas SARS-CoV-2 assay on the 6800 platform (Roche Diagnostics, Indianapolis, IN) were used to evaluate the Xpert and ID Now assays ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative PCR assays were performed using the KOD SYBR® qPCR Mix (TOYOBO) on the Light Cycler 96 system (Roche Diagnostics, Germany). ΔCt method was used for estimating transcript abundance ...
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bioRxiv - Cell Biology 2023Quote: The spaced potassium assay has been carried out as described before (Piccioli and Littleton, 2014; Roche et al., 2002; Ataman et al., 2008). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... and thiosulfohydrolase (soxB) of sulfide oxidation were quantified by SYBR-Green based qPCR assays using a LightCycler 480 II (Roche Life Science, Switzerland). Published primer pairs and a newly designed primer set for soxB were applied (Tables S1 ...
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bioRxiv - Pathology 2023Quote: Sections were stained for CD8 and pan-cytokeratin (CK) in a dual chromogenic IHC assay on a Discovery ULTRA platform (Roche Tissue Diagnostics (RTD), Tucson ...
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bioRxiv - Cancer Biology 2024Quote: ... To maintain the carryover ≤ 20% with intra-day and inter-day (2 d) CV%≤ 20% acceptable for bioanalytical assays ((Clouser-Roche et al., 2008), it was necessary to perform intermediate a post-run wash injection following each standard and sample injection ...
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bioRxiv - Biophysics 2024Quote: ... Purified motor proteins were diluted to indicated concentrations in the assay buffer with 2 mM of corresponding nucleotides (ATP-Chem Imex, ADP-Sigma Aldrich, or AMPPNP-Roche Diagnostics GmbH)fr ...
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bioRxiv - Immunology 2024Quote: ... Antibodies to the spike protein receptor binding domain and nucleocapsid were measured by the Roche Elecsys Anti-SARS-CoV-2 S and Anti-N assay (Roche Diagnostics, Indianapolis, IN), per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech) and KAPA HyperPlus Library Preparation Kit (KAPA BIOSYSTEMS). The RNA-Seq library was multiplexed and sequenced on the NextSeq 500 platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: Total genomic DNA was extracted from the bacterial pellet as described previously [28] using the PowerFood™ Microbial DNA Isolation kit (MoBio Laboratories Inc., Carlsbad, USA) and the High Pure PCR Template Preparation kit (Roche Diagnostics Ltd ...
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bioRxiv - Genomics 2020Quote: ... Library quantification was performed using the Qubit dsDNA High Sensitivity kit and quantitative PCR with the KAPA Quantification kit (Roche #KK4854) on a QuantStudio 5 real-time PCR system.
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from 10μm-sections of FFPE tissues using a commercial kit (High pure FFPET RNA isolation kit, Roche, Cat.6650775001) and resuspended in 25μl final volume ...
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bioRxiv - Microbiology 2020Quote: ... or the 5’-flanking region of Bcflp2 (amplified with Probe2For and Probe2Rev primers) using the PCR DIG Probe Synthesis Kit and the DIG Luminescent Detection Kit (Roche, Germany) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were prepared from 1 μg samples of total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) kit (KAPA Biosystems), and sequenced on the Illumina NovaSeq platform with 50-nt paired-end reads ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated chromatin was then indexed with the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences 21024) and amplified with KAPA Library Amplification Kit (Roche KK2620), before paired-end next generation sequencing.
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bioRxiv - Developmental Biology 2023Quote: Library preparation was performed with the KAPA Hyper Prep Kit for Library preparation was performed with the KAPA Hyper Prep Kit for Illumina sequencing (Cat. #KK8504, KAPA Biosystems) according to manufacturer’s guidelines with modifications ...
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bioRxiv - Neuroscience 2022Quote: ... was then transferred to a MagNaPure 96 Processing Cartridge and RNA was purified using the MagNa Pure 96 with the kit Cellular RNA Large Volume Kit (05467535001) and using the protocol “RNA Tissue FF Standard LV 3.1” (Roche Life Science). Remaining lysate was stored for later analysis of ASO contend (see below).
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bioRxiv - Immunology 2022Quote: ... mRNAseq libraries were constructed using KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin in conjunction with the KAPA mRNA Capture Kit (Roche Sequencing). Libraries were sequenced on an Illumina NextSeq500 sequencer using Illumina NextSeq 500/550 High Output v23 (150 cycles ...
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bioRxiv - Immunology 2022Quote: Libraries were cleaned up using SPRI select reagent and quantified using the High Sensitivity DNA Kit run on Agilent 2100 Bioanalyzer and also KAPA Illumina library quantification kit (Roche, 7960140001) run on LightCycler 480 ...
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bioRxiv - Neuroscience 2024Quote: Pooled libraries were evaluated using an Agilent High Sensitivity DNA kit and examined by quantitative PCR (qPCR) using the Kapa quantification kit for Illumina (Kapa Biosystems) following the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative reverse transcription-PCR (qRT-PCR) assays were performed using an ABI Prism 7900 sequence-detection system using SYBR (Roche Applied Science, Indianapolis, IN). Relative mRNA expression was calculated by the ΔCt method and the values were normalized to the expression of ARBP ...
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bioRxiv - Plant Biology 2020Quote: ... yeast strains were grown for 2 d on non-selective medium and the β-galactosidase enzymatic activity was determined in an assay using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate (Yeast Protocols Handbook ...
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bioRxiv - Biochemistry 2023Quote: ... in presence of MBP (10 μg) in the kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Biochemistry 2023Quote: ... 100 ng of recombinant PfCDPK1 protein was taken in a kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...
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bioRxiv - Genetics 2024Quote: The specificity and efficiency of the SSP set for the identification of the target scallop species (A. purpuratus) was additionally evaluated in qPCR assays performed on a LightCycler 480 II (Roche Diagnostics GmbH, Penzberg, Germany) and run in triplicate on 96-well reaction plates (Roche Diagnostics GmbH ...
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bioRxiv - Microbiology 2020Quote: A total of 100 ng of multiplex PCR amplicons were subjected to library preparation and dual-indexing using a KAPA HyperPrep Kit and a Unique Dual-Indexed Adapter Kit (Roche Applied Science) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The purified indexed PCR library products were quantified using the KAPA Library Quantification Kit from Roche (cat. no. 07960255001, kit code KK4844), normalized to a concentration of 7 nM ...
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... Final library concentrations were quantified with both Qubit fluorometric quantification (DNA dsDNA HS kit, Thermo, #Q32851) and the KAPA Library Quantification kit (Kapa Biosystems, #KK4873). The samples were run on an Agilent 2100 Bioanalyzer (Agilent #G2939BA ...
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bioRxiv - Immunology 2020Quote: ... and a quantitative Real-Time PCR was performed according to the manufacturer’s instructions (MMLV Kit, Life Technologies and Smart SYBRGreen kit, Roche Applied Science). Circadian gene expression was investigated using specific primers targeting Bmal1 and Clock genes (8) ...
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bioRxiv - Immunology 2021Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Immunology 2023Quote: ... Library yields were assessed by qPCR using the KAPA Library Quantification Kit (Complete kit, Universal) (F. Hoffmann-La Roche AG, Basel, Switzerland) on the CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad Laboratories Inc ...
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bioRxiv - Physiology 2023Quote: ... libraries were verified on a Fragment Analyzer using the High Sensitivity NGS kit (#DNF-474-0500) and quantified using the KAPA Library quantification kit (Roche, ref. KK4824).
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bioRxiv - Genomics 2022Quote: Whole genome library preparation (without duplex) Next generation sequencing libraries were generated using a double-stranded preparation kit (Kapa Hyper Prep Kit, Roche, cat#KK8502). Full-length adapters (IDT TruSeQ UDI plate ...
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bioRxiv - Molecular Biology 2024Quote: ... libraries were verified on a Fragment Analyzer using the High Sensitivity NGS kit (#DNF-474-0500) and quantified using the KAPA Library quantification kit (Roche, ref. KK4824). Following this second quality check ...
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bioRxiv - Cancer Biology 2021Quote: ... The Fast Start TaqMan® Probe Master Kit (Roche) additionally contains Taq DNA Polymerase ...
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bioRxiv - Developmental Biology 2021Quote: ... cRNA probes were generated by Digoxin labeling kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were quantified by KAPA Library Quantification Kit (Roche), normalized ...
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bioRxiv - Molecular Biology 2021Quote: ... libraries were prepared with Kapa Hyper Prep kit (Roche) according to the manufacturer’s instructions with 12 amplification cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were incubated in the Fluorescein TUNEL kit (Roche) for 1 hour at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... and visualized using the Ultraview detection kit (Roche Diagbostics) on a Benchmark XT autostainer (Ventana ...
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bioRxiv - Developmental Biology 2020Quote: ... or using the KAPA SYBR FAST kit (KAPA Biosystems) on a Bio-Rad CFX96 machine ...
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bioRxiv - Developmental Biology 2022Quote: ... and transcribed with a DIG-labelling Kit (Roche, #11277073910) using T3 polymerase (Roche ...
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bioRxiv - Immunology 2020Quote: ... Using KAPA HiFi HotStart ReadyMix PCR Kit (Kapa Biosystems) the two 5’UTRs were amplified from cDNA.