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Citations for Roche :
1201 - 1250 of 1968 citations for IL 2 Rat HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... pyruvate supplemented with 2% Bovine Serum Albumin Fraction V Fatty acid free (Roche, USA/UK), 50 µM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μL of 10x PCR Reaction Buffer with MgCl2 (Roche Life Science, Indianapolis, IN, USA), 0.15 μL Taq DNA Polymerase (Roche Life Science ...
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bioRxiv - Cancer Biology 2019Quote: ... NK-92 cultures were supplemented with 150 IU/ml rhIL-2 (Hoffman-La Roche Inc.). The amphotropic-packaging 293T and Phoenix cells were cultured in Dulbecco modified Eagle medium (DMEM) ...
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bioRxiv - Cell Biology 2020Quote: ... 9ml of cold wash buffer (PBS, BSA 2% and 0.2U/μl RNase inhibitor from Roche) was added and the lysate was dounced with 10 strokes of loose pestle avoiding too much pressure and air bubbles ...
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bioRxiv - Biochemistry 2019Quote: ... 20% glycerol) containing 0.5 μM 2-mercaptethanol (Fujifilm Wako) and protease inhibitor (Roche, Basel, Switzerland) and solubilized with 0.6% (w/v ...
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bioRxiv - Physiology 2019Quote: ... Glomus cells were dissociated using a mixture of collagenase P (2 mg/ml; Roche, USA), DNase (15μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... mRNA expression analysis was determined using KAPA SYBR Fast Master Mix (2×) Universal (KAPA Biosystems) in an Eco Real-Time PCR System (Illumina) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2% Triton X-100 and 274 mM NaCl) containing a cocktail of protease inhibitors (Roche). Cells were lysed for 20 min on ice and centrifuged for 15 min at 17,400 r.p.m ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001) and thermal cycler program ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM MgCl2 (buffer B) containing 2 mM PMSF and Complete protease inhibitor cocktail (Roche). After centrifugation ...
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bioRxiv - Genetics 2021Quote: ... 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche) was used to resuspend the cells ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were counterstained with 5 ng/ml of 4,6–diamidino-2-phenylindole dihydrochloride (DAPI; Roche). Coverslips were mounted on slides with Fluoromount G (Electron Microscopy Sciences ...
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bioRxiv - Immunology 2020Quote: ... diluted 2-fold with PBMC culture media and supplemented with 10% WST-1 reagent (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and the RNA was subsequently precipitated in 2-propanol and 20 μg/μl glycogen (Roche) overnight at −20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... femurs were cut roughly and incubated with 2 Wunsch units of LiberaseTM (Sigma/Roche 5401127001) and 1 mg of Pronase (Sigma/Roche 10165921001 ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM PMSF) supplemented with 1 protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Genetics 2022Quote: ... sections were incubated with DAPI (4′,6-diamidino-2-phenylindole dihydrochloride, Roche, 1:250 dilution) for 10 sec and washed in PBS.
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bioRxiv - Biophysics 2023Quote: ... 2 mM PMSF) supplemented with one protease inhibitor cocktail tablet (cOmplete-EDTA free, Roche, #11836170001) to a final volume of 50 mL ...
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bioRxiv - Immunology 2023Quote: Anti-nucleocapsid IgG was measured using the Elecsys Anti-SARS-COV-2 assay (Roche; 09203095190) run on a Cobas e411 analyser (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 2 ng of cDNA was amplified per reaction in a 364-well plate (4729749001, Roche) with LightCycler® 480 SYBR Green I Master (4887352001 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3h at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... was diluted 1 in 50 in solution consisting of 2 % blocking reagent (Roche ref 11096176001), 1 % goat serum (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM TCEP and 5 % glycerol) supplemented with cOmplete EDTA-free protease inhibitor complex (Roche) and ribonuclease A (Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μg/mL DAPI (4,6-diamidino-2-phenylindole, F. Hoffmann-La Roche, Natley, NJ, USA) was added to counterstain the nuclei ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then digested with Liberase Blendzyme 2 (0.2 mg/ml, Roche Applied Science) and DNase I (20 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated in complete RPMI-10 supplemented with 50U/mL hIL-2 (Roche 11011456001), 2.5ng/mL TGFβ (R&D 240-B-10) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) supplemented with 2% v/v protease inhibitor cocktail (Roche; Catalogue #11697498001) and 1% v/v phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Roche). To reduce autofluorescence ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM beta-mercaptoethanol) in the presence of a protease inhibitor cocktail (Roche, Basel, Switzerland). These mixtures were incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... was amplified using F3 and R3 primers (Supp. Table 2) and KAPA-Hifi polymerase (Roche). Following a 1X SPRIselect bead DNA cleanup (Beckman Coulter) ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were incubated with 2% Dispase II (neutral protease; Roche Applied Science, Indianapolis, IN) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclear pellets were washed once with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) (Roche, 10236276001) in methanol (1 μg/ml) ...
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bioRxiv - Immunology 2024Quote: ... 2 mL aliquots of peripheral blood were treated with 30mL of RBC lysis buffer (Roche) for 5-8 minutes at room temperature with gentle mixing ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM PMSF and 1 tablet/80 ml of complete EDTA-free protease inhibitor cocktail (Roche)] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed in ice-cold FACS buffer (2% FBS, (0.5 ug/ml) DNase I (Roche), 2 mM EDTA in PBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... Library amplification and indexing were performed with KAPA HiFi HotStart Uracil+ ReadyMix (2×) (Kapa Biosystems KK2801). The PCR amplification was carried out as follows ...
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bioRxiv - Genomics 2019Quote: ... The remaining fragments were then treated with a collagenase/dispase mixture (2 mg/mL final) (Roche) and DNase I (2 mg/mL final ...
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bioRxiv - Genomics 2020Quote: ... contaminating genomic DNA was removed by incubating with 2 µl of RNase-free DNase I (Roche) for 30 min at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... with both basic (Cell Conditioner 1) and mildly acidic (Cell Conditioner 2) antigen retrieval methods (Roche, Ventana Medical Systems ...
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bioRxiv - Biophysics 2019Quote: ... (5) motor solution consisting of 2 μM biotinylated KIF1A in motor dilution buffer (1mM ATP [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µL of sample was mixed with 10 µL 2X SYBR Mix (Kapa Biosystems, Wilmington, MA), 0.4 µL of each 10 µM primer (IDT ...
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bioRxiv - Microbiology 2021Quote: ... spanning the SARS-CoV-2 genome) were purified using Kapa HyperPure beads (Roche Molecular Systems Inc) and quantified using a Qubit fluorometer and dsDNA HS Assay Kit (Thermo Fisher Inc ...
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bioRxiv - Pathology 2021Quote: ... Lymph node samples then had 2 µl of 500 µg/mL of proteinase K (PK, Roche) (diluted in 1x PBS and 0.5 M EDTA ...
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bioRxiv - Plant Biology 2020Quote: ... 2 µg of RNA was reverse-transcribed with Transcriptor First Strand cDNA synthesis Kit (Roche, www.roche.com). The A ...
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bioRxiv - Microbiology 2021Quote: ... DS6350 chromosomal DNA was used as a template and Expand polymerase with Expand Buffer 2 (Roche). The resulting PCR fragment was purified using the QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested and resuspended in 2 ml of 1X PBS containing protease inhibitor cocktail (Roche) and 500μl of resuspended cells was taken as a control for uncrosslinked sample (referred to as -DSG in the text) ...
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bioRxiv - Microbiology 2020Quote: ... but tested negative on the day of sampling (cobas® SARS-CoV-2 test, Roche diagnostics), and from a healthy individual ...
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bioRxiv - Neuroscience 2020Quote: ... All the PCR reactions were carried out with Kapa HiFi 2 x mastermix (KK2601, Kapa Biosystems). PCR products were then separated by 2% agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of each plasmid was combined with Fugene transfection reagent (Roche Applied Science, Mannheim, Germany) at a ratio of 5 µl of transfection reagent to 1 µg DNA in 500 µl of MEM and incubated at room temperature for 30 minutes prior to addition to a 10 cm dish of HEK293 cells at 50% confluence ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were incubated in 1μg/ml of 4′,6-diamidino-2- 532 phenylindole (Roche, Cat# 10236276001) at RT for 10min and washed in PBS 1x ...