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Citations for Roche :
1201 - 1250 of 1744 citations for 6 BENZOFURAN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... resuspended in ice-cold homogenizing buffer (20 mM HEPES pH 7.4, 250 mM sucrose, 2 mM MgCl2, and EDTA-free protease inhibitor cocktail [Roche]), and homogenized on ice by 25 passages through a 26-guage needle fitted to a 1 mL syringe ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 4 hr and washed two times with its resuspending buffer and once with E100 buffer (25 mM HEPES-KOH pH7.5, 10 % glycerol, 100 mM KCl, 2 mM MgCl2, 1 mM EDTA with Roche cOmplete™ ...
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bioRxiv - Immunology 2020Quote: ... The red blood cell layer was lysed with 2 mL of Red Blood Cell Lysis Buffer (Cat# 11814389001, Roche) in a 15 mL tube ...
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bioRxiv - Biophysics 2020Quote: ... N2A cells were transfected with 1.0 μg of a cDNA construct and 2 μL of X-tremeGENE HP DNA transfection reagent (Roche Diagnostics GmbH ...
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bioRxiv - Cell Biology 2021Quote: To isolate mammary epithelial cells the third and fourth pairs of mammary glands were removed from 4 to 6-week-old s-SHIP–GFP transgenic (Tg11.5kb–GFP) mice, minced with scissors and digested in digestion medium (DMEM/F12, 2 mg ml−1collagenase I (Roche), 5 mg ml−1 insulin (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were isolated by adding SDS lysis buffer (50 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol) supplemented with protease inhibitor cocktail (PIC; Roche). DNA was sheared by sonication for 10 min at high settings (30 s on ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended in 1x sample buffer (0.5 x TBE, 10% glycerol, 2% SDS) supplemented with protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail (5 mM sodium fluoride ...
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bioRxiv - Plant Biology 2020Quote: ... Cell pellets from 200 mL of Chlamydomonas culture were resuspended in an extraction buffer (20 mM HEPES pH= 7.5, 20 mM KCl, 10% glycerol, 2× EDTA-free protease inhibitor mix (ROCHE)) ...
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bioRxiv - Cell Biology 2021Quote: ... diluted with an equal volume of IP buffer (150 mM HEPES-KOH pH 6.8, 150 mM K2OAc, 2 mM MgOAc, 1 mM CaCl2, 15% glycerol, 1% NP-40, 1x Roche protease inhibitor cocktail and 1 x phosSTOP cocktail ...
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bioRxiv - Microbiology 2020Quote: ... pellets were re-suspended in 1.2 mL of Lysis Buffer (50 μg/ml lysozyme, 0.8% NP-40, 1 mM MgCl2, 1x protease inhibitors [Roche; cOmplete Protease Inhibitor Cocktail] in PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... 500 mM Tris pH 7.4, 20 mM EDTA, 10 mM NaF, 2 mM benzamidin and protease inhibitor cocktail [Roche]), and centrifuged 3 min at 5000 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Peels were cut in 2-5 mm2 pieces and placed in 5 ml digestion solution (0.75 mg/ml Liberase TH Research grade (Roche), 0.1 mg/ml DNAseI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the small intestines were cut into 2-5mm2 pieces and put into digestion solution (0.75mg/ml Liberase TH Research Grade (Roche), 12 U/ml Dispase ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellet samples were collected and re-suspended in lysis buffer (100 mM Tris-HCl, pH 7.4, 100 mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA, Roche complete protease inhibitor set ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT, 1 mM PMSF, 26.3 μM MG-132 [Chemscene, New Jersey, USA], 2 cOmplete EDTA-free protease inhibitors [Roche, Basel ...
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bioRxiv - Neuroscience 2022Quote: ... Pronase solution was removed and 2 ml of reconstitution solution (1% fetal bovine serum + DNAse (1:10,000, Roche #47167280001)) in oxygenated ACSF was added ...
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bioRxiv - Genomics 2022Quote: ... Beads were washed twice 10 min with cold buffer I (150 mM NaCl, 1% Triton X-100, 0.1% SDS, 2 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biophysics 2022Quote: ... For cell lysis the cells were resuspended in 4 mL per g pellet in lysis buffer (20 mM Tris/HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, Protease Inhibitor (ROCHE), spatula DNAseI ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissues were manually minced and digested in 25 mL of Dulbecco’s Modified Eagle Medium (DMEM) containing 2 mg/mL of collagenase A (Roche) and DNase I (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of bromophenol blue and 50 mM DTT) containing 2 mM PMSF and cOmplete protease inhibitor cocktail (Roche) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Whole cerebral organoids were washed three times in 1× PBS and dissociated in 2 mL of Accutase (StemPro) containing 0.2 U/μL Dnase I (Roche) for 45 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... before treating with lysis buffer (10 mM Tris pH 7.5, 2 mM EDTA) containing a protease and phosphatase inhibitor cocktail (Roche). Chromatin-enriched protein fractions were prepared essentially as described (Mailand and Diffley ...
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bioRxiv - Neuroscience 2021Quote: Proteins were extracted from one brain hemisphere or from purified MVs in 2% SDS (500 µl or 50µl per sample, respectively) with EDTA-free Complete Protease Inhibitor (Roche), sonicated three times at 20 Hz (Vibra cell VCX130 ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X- 100, 2 mM EDTA, 20 mM Tris, pH 8.1, 150 mM NaCl, and protease inhibitors; Roche), and then washed once with 1 ml of “high-salt buffer” (the same but with 500 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Platelet activation was achieved by complementing the Tyrodes buffer with 2 mM Ca2+ and the addition of 0.01 U/ml Thrombin (10602400001, Roche) to the platelet suspension.
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bioRxiv - Neuroscience 2022Quote: ... were mixed with an equal volume of 100 mmol/L Tris-HCl (pH 7.4) – 2% sarkosyl for 30 minutes prior to PK (200 μg/mL; Roche) digestion for 1 h at 37° C ...
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bioRxiv - Microbiology 2022Quote: ... 1.5 million S2 cells were transfected with 2 μg of plasmid using Xtreme-GENE HP DNA transfection reagent (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was diluted 1:5 in PCR-grade dH2O and 1.5 µl of the cDNA-mix was used per 20 µl real-time PCR reaction with 0.25 µM of each primer and 2 x FastStart Essential DNA Green Master Mix (Roche). For each target and reference gene ...
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bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in buffer1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2, Protease inhibitors (Roche)) and incubated for 20 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... washed with PBS and suspended in Ni-wash buffer (20 mM Tris/HCl pH 8, 10 mM imidazole, 200 mM NaCl, 10 mM DTT, 2 mM PMSF, 0.5x Roche Protease Inhibitor cocktail ...
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bioRxiv - Genetics 2020Quote: ... and one day later 2 micrograms of plasmid DNA was transfected into cells using Xtremegene HP transfection reagent (Roche) according to the manufacturer’s specifications ...
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bioRxiv - Genetics 2021Quote: ... Isolation of cfDNA from 2 mL of plasma was performed using the semi-automated MagNA Pure 24 System (Roche) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The sections were then rinsed with 2× SSC and 0.2× SSC and treated with alkaline phosphatase– conjugated anti-digoxigenin (anti-DIG, 1:500; Roche) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.5, 150 mM NaCl, 5 mM EDTA, 2 mM ATP, 1 mM dithiothreitol and protease inhibitor cocktail tablets; Roche) using Bead Beater ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and lysed by sonication in prenylation buffer (50 mm HEPES, pH 7.2, 50 mm NaCl, 2 mm MgCl2, 100 μm GDP, 1× Roche complete EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Pathology 2021Quote: ... adjust pH to 9.5 adding HCl) for 2 minutes and then tissue was covered with NBT/BCIP stock solution (Roche) diluted in 0.1M TRIS buffer (pH 9.5 ...
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bioRxiv - Genetics 2021Quote: ... Bacteria were pelleted and resuspended in lysis buffer (50 mM Tris pH 8.8, 200 mM NaCl, 2 mM DTT, 1 mM PMSF, 1 mg/ml lysozyme, 1x Roche cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets resuspended in LB1 (10 mM Tris-HCl pH 7.5, 2 mM MgCl2, 3 mM CaCl2 supplemented with protease inhibitors [Roche]) for 5 minutes at 4 °C followed by centrifugation (1,000g ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl PH8.0, 150 mM NaCl, complete protease inhibitor [Roche]), high salt (0.01% SDS ...
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bioRxiv - Molecular Biology 2019Quote: For the generation of mass spectrometry samples the beads were washed 4 times with EBC-2 buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, and protease inhibitor cocktail (Roche)) and 2 times with 50 mM ammonium bicarbonate followed by overnight digestion using 2.5 µg trypsin at 37 °C under constant shaking ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]) ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissue was homogenized in 2 ml buffer (100 mM Tris pH8, 150 mM NaCl, 0.5% TX-100, protease inhibitor cocktail (Roche)) per gram of tissue ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in Buffer A (2 M NaCl, 20 mM HEPES pH 7.5, 20 mM Imidazole, 5 mM βME, protease inhibitor (Roche)) by sonication ...
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bioRxiv - Genomics 2019Quote: ... 12000g and supernatant diluted with 1 volume of Dilution Buffer (1mM EGTA 300 mM NaCl, 2% Triton x-100, 0.2% sodium deoxycholate, 1x protease inhibitors-Roche). Sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 6 ug of antibody conjugated to 20 µl of Dynabeads ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris/HCl pH 7.4/ 50 mM NaF, 10 mM Na4P2O7, 2 mM MgCl2 and Complete Mini, EDTA-free protease inhibitor [Roche]). Lysates were cleared and incubated with GFP-Trap agarose beads for 60 min ...
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bioRxiv - Neuroscience 2020Quote: ... The beads were then washed 2 × with washing buffer (20 mM Tris, 150 mM NaCl, 0.5 % Triton-X-100, 2 mM EGTA, complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were dissociated in 1-2 ml of digestion mix (D-PBS, 250ug/ml Liberase TL (Roche; cat: 5401020001), 0.1 mg/ml DNaseI (Sigma ...
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bioRxiv - Physiology 2021Quote: ... Muscles were homogenized in buffer (2% sodium dodecyl sulfate, 50□mM Tris-HCl, pH 6.8) with protease and phosphatase inhibitors (Roche). Protein concentrations were determined by Bicinchoninic acid assay (Sigma-Aldrich ...
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bioRxiv - Physiology 2020Quote: ... were sonicated in lysis buffer (2% SDS, 60 mM Tris-HCl, pH 6.8) with phosphatase and protease inhibitors (Roche). Protein concentration was measured using a BCA assay (Pierce) ...