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Citations for Roche :
1201 - 1250 of 1647 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 100 μl of MNase buffer (0.3 M Sucrose, 85 mM Tris, 3 mM MgCl2, 2 mM CaCl2, 2.5U of micrococcal nuclease: Roche 10107921001) was added in to each tube (0.5 millions of cells per tube) ...
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bioRxiv - Developmental Biology 2024Quote: ... whole-exome DNA from blood or salivary samples was captured using the Nimblegen v.2 exome capture reagent (Roche) or Nimblegen SeqxCap EZ MedExome Target Enrichment Kit (Roche) ...
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bioRxiv - Immunology 2024Quote: Five female mosquitoes were collected in 250 μL of 2× RIPA buffer complemented with protease inhibitor (Complete 1×, Roche) in tubes containing ∼20 1-mm glass beads (BioSpec) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated in 50% Hybridization Buffer (50% formamide, 2x SSC, 1 mg/ml Torula RNA, 0.05 mg/ml Heparin, 2% Roche blocking reagent ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 μg of total RNA was retro-transcribed using Transcriptor First Strand cDNA Synthesis Kit (Roche; Cat #04897030001) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in 200 μL lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, cOmplete Protease Inhibitor Cocktail, Roche), heated to 99 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was washed five times with 10 CV of Amylose Buffer (30 mM HEPES pH 7.6, 0.3 M NaCl, 0.1% CHAPS, 0.25 mM EDTA, 2 mM DTT, 10% glycerol, 1× Roche cOmplete protease inhibitors). Protein was eluted with Amylose Buffer supplemented with 20 mM maltose ...
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bioRxiv - Physiology 2024Quote: Fibroblast proliferation was assessed using a 5-bromo-2’-deoxyuridine (BrdU) incorporation assay (colorimetric) from Roche (Indianapolis, IN, USA) for 48 h following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The total lysate was clarified by centrifugation at 85,000×g for 30 minutes and then mixed with 2 mL cOmplete His-tag NiNTA beads (Roche) pre-equilibrated in purification buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were analyzed for glucose production rate at 2 h by using an Accu-Chek® Aviva glucometer (Roche Diagnostics Scandinavia AB ...
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bioRxiv - Biophysics 2024Quote: ... 2 million cells were seeded in a 10-cm cell culture dish for transfection using X-tremegene 9 (Roche) and Opti-MEM (Gibco ...
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Conserved Cis-Acting Range Extender Element Mediates Extreme Long-Range Enhancer Activity in MammalsbioRxiv - Genomics 2024Quote: ... embryos were with PBT (x3, 15mins) and incubated in prehybridization buffer (50% deionized formamide, 5× SSC, pH 4.5, 2% Roche Blocking Reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... SDS (0.2%), Triton X-100 (2%), sodium deoxycholate (1%), Tris HCl (100 mM, pH 8.0)) with protease inhibitor (Roche #11836145001), then incubated on ice for 30 m ...
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bioRxiv - Microbiology 2024Quote: ... Broncholalveolar lavage (BAL) fluids were obtained by intratracheal injection of 2 × 1 ml PBS supplemented with protease inhibitors (Roche). Serum was collected by intracardiac puncture and separated from whole blood in Z-Gel microtubes (Sarstedt) ...
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bioRxiv - Neuroscience 2024Quote: ... The ganglia were minced with iridectomy scissors into fine pieces and then transferred to a 35 mm petri dish containing 2 ml enzyme mix (0.4 mg/ml Trypsin (Roche), 1.2 mg/ml collagenase D (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... in nuclei resuspension buffer of 1x PBS with 2% BSA and 0.2 U/µl Protector RNase Inhibitor (Roche, USA). Resuspended nuclei were then overlaid on 750 μl of 29% Optiprep in nuclei resuspension buffer before centrifuging at 13,000 g for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and incubated on ice for 1 hour and washed thrice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... and reversely transfected with 0.2 µg HiBIT –tagged SETDB1 (3TD) and 1.8 µg of empty plasmid using X-tremeGene XP transfection reagent (Roche), following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... cells were re-suspended and lysed in ice-cold RIPA buffer (50 mM Tris pH8, 150 mM NaCl, 2 mM EDTA, 0.5% NP40) with protease inhibitor cocktail (Roche) and sonicated ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)) supplemented with cOmplete™ EDTA-free Protease Inhibitor Cocktail Tablets (Roche) and lysed by sonication ...
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bioRxiv - Biochemistry 2024Quote: ... and pellets were resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]). The cells were lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 0.5 mM TCEP, 1× Benzonase, 2× protease inhibitor cocktail [Roche cOmplete]), and lysed using a cell disruptor at 1.5 kbar ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were resuspended in lysis buffer (25 mM HEPES pH 7.4, 2 mM EGTA) supplemented with EDTA-free cOmplete protease inhibitor cocktail (Roche). Cells were lysed by five passages through a chilled Emulsiflex C3 homogenizer (Avestin) ...
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bioRxiv - Cell Biology 2024Quote: ... and subsequent incubation with 80 units/mL collagenase II and 2 units/mL dispase II (catalog number: 04942078001, Roche) solution ...
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bioRxiv - Cancer Biology 2024Quote: ... or SDS extraction buffer (2% SDS, 50 mM Tris pH 6.8) with freshly added Complete protease Inhibitor Cocktail (Roche). Extracts were run on 10% polyacrylamide SDS-PAGE gels ...
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bioRxiv - Cell Biology 2024Quote: ... 22μl H2O) before the addition of 25μl amplification mix 2 (0.75μl Expand high fidelity enzyme mix (Roche, cat. 11732641001), 5μl 10X Expand high fidelity buffer with 15mM MgCl2 (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were thawed and resuspended in 10 µL benzonase buffer (50 mM Tris-HCl pH 8.0, 2 mM MgCl2) with EDTA-free protease inhibitor (Roche, 11873580001 ...
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bioRxiv - Biochemistry 2020Quote: For Figures 2 and 6: Cells or embryos were lysed in RIPA buffer supplemented with a cocktail of protease inhibitor (Roche). Total protein was resolved on SDS polyacrylamide gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 100 mL of cold lysis buffer (50 mM HEPES pH 8, 175 mM NaCl, 5 % glycerol, 1 mM EDTA and 2 tablets of protease inhibitors by Roche). The bacterial solution was then incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by addition of 5 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (i.e., MTT) using a Cell Proliferation Kit I (Roche Diagnostics, Mannheim, Germany), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cell viability was determined by MTT (C, N-diphenyl-N′-4,5-dimethyl thiazol-2-yl tetrazolium bromide) assay (Roche, Switzerland), a standard colorimetric assay which uses the metabolic reduction of the tetrazolium salt to form the colored formazan product [33] ...
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bioRxiv - Immunology 2021Quote: ... S1 protein and CP15 were formulated in nanoparticles in PLGA (Alchem Laboratories) for the first 2 doses and the last (one-year) boost was in DOTAP (100 μl per dose; Roche). For immunization ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche, Manheim, Germany).
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bioRxiv - Developmental Biology 2021Quote: After blocking in 20% heat-inactivated sheep serum (Cat. # ZLI-9021, Beijing Zhongshan Jinqiao Biotechnology Company) and 2% blocking reagent (Cat. # 12039672910, Roche) for 1 h ...
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bioRxiv - Genetics 2022Quote: ... 10 µl of each PCR product were run in 2% agarose gels alongside digoxigenin (DIG)-labeled size markers VII and VIII (Roche), for 16 hours at 50 V then transferred to a positively charged nylon membrane (Roche ...
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bioRxiv - Systems Biology 2022Quote: ... The oligonucleotide pool was further amplified by mixing 12.5 μl KAPA HiFi HotStart 2× ReadyMix (KAPA Biosystems cat. no. KK2601), 0.75 μl of 10 μM forward primer ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 mM Tris pH 8.0, 0.05% SDS, 2 mM MgCl2, 10 U/ml benzonase, and cOmplete™ EDTA-free protease inhibitor cocktail [Roche, 27368400]). Extracts were then incubated on ice for 10 min before protein concentration was calculated by Bradford assay (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: T98G cell pellets were fixed by dropwise addition of cold 70% ethanol and their DNA was stained with 4’,6-diamino-2-phenylindole (1 μg/ml, Roche), 0.1% Triton X-100 in phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sections were blocked for 1 h with 2% blocking reagent in MABT (Perkin-Elmer) and then incubated with anti-digoxigenin-POD (Roche), diluted 1:500 ...
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bioRxiv - Genomics 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate with 2 μL of cDNA (1:4 dilution) per reaction using the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
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bioRxiv - Developmental Biology 2021Quote: ... Template DNAs of interest (500 ng each) were transcribed in vitro during 2 hours at 37°C using SP6 RNA polymerase kit (Roche), followed by a 1 hr treatment with DNAse I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... Samples were analyzed according to the manufacturer’s guidelines using the Elecsys Anti-SARS-CoV-2 assay on the Roche cobas e602 platform (Roche Diagnostics). A cut-off index (COI ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with Glutamax and 10 % FBS were transfected using a 2:1 ratio X-treme GENE HP DNA transfection reagent (Roche) per μg of plasmid DNA with a total of 0.5 μg DNA per well ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using 2-5 times the volume of RIPA lysis buffer supplemented with 1x protease inhibitor cocktail (Roche), PMSF (1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell pellet was subjected to a freezing-thawing step and resuspended in the lysis buffer [2% Nonidet P40 (NP40; 11332473001, Roche), 0.2% SDS (75746 ...