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Citations for Roche :
1101 - 1150 of 10000+ citations for Retinol Binding Protein RBP Multi format ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Each reaction contained a 5μl of a 1 in 10 dilution of genomic DNA and was carried out in quadruplicate in a volume of 15 μl in a 384-well LightCycler® 480 PCR plates (Roche), sealed with LightCycler® 480 sealing foil (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... All reactions were performed in technical triplicate using 384-well plates on the LightCycler 480 System using the thermal cycling conditions as per the manufacturer’s instructions (Roche Diagnostics GmbH). The 2^DDCt method was used to calculate relative changes in gene expression and normalization to the arithmetic mean of both B2M and TBP.
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bioRxiv - Genetics 2021Quote: ... and the internal control vector TK (25 ng per well for 48-well plate) were co-transfected into the cells by using the X-tremeGene HP DNA transfection reagent (ROCHE, 6366236001). The transfected HEK293T cells were harvested in 65 μL passive lysis buffer (Promega ...
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bioRxiv - Cancer Biology 2021Quote: 1 μl sample from Denaturation was transferred to the qPCR plate well containing the qPCR Mix (Nuclease-free water + KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) + cDNA Primers (from Visium Reagent kit)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were loaded into 96-well plates and were performed at 63 °C during 35 min using a LightCycler 96 Real-Time System (LC96) (Roche Diagnostics). One melting cycle was performed at 0.1 °C/s from 63 °C up to 97 °C for validation of the specificity of the amplified products ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well of cells from 24-well plates was lysed with 120 μL of RIPA buffer with protease inhibitor (Roche, 11836153001). All protein lysates were denatured with 12% β-mercapto-ethanol in 6x SDS-loading dye ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection was performed on six-well plates or confocal dishes for different purposes with plasmids using X-tremeGENE HP DNA Transfection Reagent (Roche, 6366236001) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed in 384-well plates with a total volume of 10 μL and the reaction mixtures were run on a LightCycler 480 system (Roche, Germany). Data are shown as the relative mRNA expression compared to 0% MgCl2 test with the endogenous reference 16S rRNA gene.
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bioRxiv - Immunology 2024Quote: ... sciatic nerves and brains were crushed with the back of a 3ml syringe in a serrated 24 well plate and triturated in digestion media (Liberase TM (0.208 WU/ml) (Roche Cat # 054010200001) and DNase I (40 ug/ml ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were then triturated in a 1:1 mixture of 1 mg/mL trypsin inhibitor (Roche) and bovine serum albumin (BioPharm Laboratories) ...
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bioRxiv - Microbiology 2024Quote: ... or HA (Cell signaling, 3724, 1:1,000; Cell signaling, 2999, 1:1,000; Roche, 54732500, 1:1,000). Secondary horseradish peroxidase (HRP)-conjugated antisera recognizing rabbit ...
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bioRxiv - Microbiology 2022Quote: ... 150 mM NaCl; 1 mM EDTA; 1 mM CaCl2; 1 mM MgCl2; 1% Triton, 0.5% sodium deoxycholate; protease inhibitor cocktail [Roche] one tablet/10 mL). The cells suspension was incubated on ice for 30 min and then centrifuged at 13,000 rpm/15 min/4°C ...
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bioRxiv - Plant Biology 2019Quote: ... one with the Illumina Genomic DNA Sample Prep kit and one with the Kapa Hyper kit (Kapa Biosystem, Roche). Three mate-pair libraries were constructed using Illumina Nextera Mate Pair Library Prep kit with insert sizes ranging between 2-4 kb ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were validated for quality on Agilent DNA1000 Kit and quantified with the qPCR NGS Library Quantification kit (Roche). The final libraries were equimolar pooled and sequenced using the High Output 1×75 kit on the Illumina NextSeq500 platform producing 75bp single-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by in vitro transcription with DIG-RNA labelling Kit or Fluorescein-RNA labelling kit (Roche, Switzerland). The integrity of probes was determined by gel electrophoresis and storage in 50% RNAlater (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Capture library concentrations were determined by qPCR with a KAPA qPCR kit (KAPA Library Quant Kit, Kapa Biosystems, USA) and pooled at the same concentration for sequencing ...
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bioRxiv - Genetics 2021Quote: ... Capture-C libraries were made using the Arima HiC kit (Arima Genomics) and the KAPA HyperPrep kit (KAPA Biosystems) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The absence of kit/reagent contamination was verified in the High Pure Viral Nucleic Acid Kit (Roche Applied Science) and Illustra™ GenomiPhi V2 DNA Amplification Kit (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... and the second-strand cDNA was synthesized using a KAPA Biosystems kit (Roche KAPA HiFi Hotstart PCR kit, KK2502) with VHgene-specific primers (No ...
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bioRxiv - Biochemistry 2024Quote: ... An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems, Wilmington, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:10,000, Sigma #F1804-5MG; anti-GroEL, 1:10,000, Sigma; or anti-GFP, 1:10,000, Roche #11814460001 in 3% bovine serum albumin (BSA)/TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... and lysozyme (1 ml, 10 mg ml-1; Roche, Switzerland) were added to remove the OM and cell wall respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Triton X-100) + 1% SDS + PIC (Roche 05056489001). Sonication was performed in a BioRuptor (Diagenode ...
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bioRxiv - Neuroscience 2020Quote: ... 1% NP-40 and 1× protease inhibitor cocktail (cOmplete, Roche), phosphatase inhibitor cocktails I and II (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA and 1% CompleteTM protease inhibitor cocktail (Roche)) by passing the cell mixture through a 26-gauge needle at 4°C (50-100 strokes) ...
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bioRxiv - Genomics 2020Quote: ... 1 protease inhibitor tablet (Roche Complete cat #1 697 498) per 50ml buffer) ...
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bioRxiv - Plant Biology 2019Quote: ... blocked in 1×TBS-T with 1% Blocking Reagent (Roche) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 1% NP-40 and 1× complete protease inhibitor cocktail (Roche)) by three rounds of chopping the tissue using dissection scissors and a handheld homogenizer7 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM DTT) + protease inhibitors (1 tablet / 50 ml Roche Complete Ultra EDTA-free ...
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bioRxiv - Neuroscience 2023Quote: ... 1% SDS solution after adding protease inhibitor (Roche 4693116001; 1×) and phosphatase inhibitor cocktails (Roche 4906845001 ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2019Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Immunology 2019Quote: ... The protein was produced by transient transfection of HEK 293T cells with XtremeGene™ HP Transfection reagent (Roche) according to the manufacturer’s instructions and purified following a published protocol (77) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
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bioRxiv - Biochemistry 2019Quote: Overall protein was extracted through RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) with protease inhibitors (Roche, Basel, Switzerland), and then quantified through BCA™ Protein Assay Kit (Pierce ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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Individual differences in honey bee behavior enabled by plasticity in brain gene regulatory networksbioRxiv - Genomics 2020Quote: ... with protein inhibitor complex (PIC, Complete Tablets, EDTA-free Protease Inhibitor Cocktail from Roche, Basel, Switzerland, cat. #04693132001) using a motorized pestle for 20 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent amounts of protein samples were separated by SDS-PAGE and electroblotted onto a polyvinylidene fluoride membrane (Roche) using a Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... shTDP-43 HEK293E cell pellets from confluent 10cm plates were washed twice in ice-cold PBS and then resuspended in 500μl of hypotonic buffer A (10mM HEPES, 1.5mM MgCl2, 10mM KCl, 0.1mM DTT and protein inhibitor cocktail (Roche) and incubated on ice for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: Proteins from various cells were extracted with 1x RIPA buffer containing 1x complete-mini protease inhibitor (Roche; #11836170001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2020Quote: Total proteins were extracted from mouse striata in PBS containing 0.1% Tween 20 and Protease Inhibitor cocktail (Roche) with or without 1 mM non-hydrolysable ADP (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10, Roche) or chicken anti-c-Myc primary antibody (A2128 ...
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bioRxiv - Cancer Biology 2019Quote: All A673/TR/shEF in vitro and xenograft proteins were extracted with RIPA and anti-protease cocktail (Roche). Western blots were hybridized with rabbit monoclonal anti-FLI1 antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...