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Citations for Roche :
1051 - 1100 of 2228 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 2 mM DTT) supplemented with a cocktail of protease and phosphatase inhibitors (Roche). The samples were kept on ice for 30 min and flash frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 12.5 μl 2× Kapa HiFi HotStart Ready Mix (Kapa Biosystems, Wilmington, MA, USA) and PCR-grade water ad 25 μl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II ...
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bioRxiv - Neuroscience 2022Quote: ... An enzyme mixture (0.5 ml) consisting of dispase II (2 mg/ml; Roche, Mannheim ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM dithiothreitol [DTT]) supplemented with cOmplete EDTA-free protease inhibitor mixture (Roche), 1 mg/mL lysozyme (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 12.5 µl of 2× KAPA2G Robust HotStart ReadyMix (Kapa Biosystems, Wilmington, MA, USA), and 0.2 µM of each primer in a total volume of 25 µl ...
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bioRxiv - Microbiology 2023Quote: ... 1X antibiotic-antimycotic) with 100 U/mL recombinant interleukin (IL)-2 (Roche #11147528001) and activated or frozen in freezing media (90% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM DTT and 2 tablets of Complete Protease Inhibitor Cocktail Tablets (Roche) per 100 ml lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... were stained overnight with 4′,6-diamidino-2-phenylindole (DAPI, Roche, Basel, Switzerland) at a final concentration of 5 µg ml-1 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 % Glycerol) supplemented with one tablet of EDTA-free protease inhibitor cocktail (Roche). After sonication (4x 6 min ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in a saturation solution containing 2% Blocking Reagent (Roche Diagnostics) and 20% sheep serum in MABT for 4h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Basal respiration was measured in DMEM containing 2% fatty-acid-free BSA (Roche). Cells were treated with 100 μM isoproterenol to stimulate respiration ...
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bioRxiv - Cancer Biology 2023Quote: ... erythrocytes were lysed in 2 mL red blood cell lysis buffer (Roche, 11814389001) for 5 min at room temperature before the addition of 10mL AdDF+++ and centrifugation at 400 rcf ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cell proliferation ELISA BrdU (5′-bromo-2′-deoxyuridine) colorimetric assay (Roche,11647229001) was performed as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2% bovine serum albumin (BSA; fraction V, fatty-acid-free; Roche), 50 µM β-mercaptoethanol (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Primers were designed using the Universal ProbeLibrary Assay Design Center (Roche, Tab. 2). Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Articular cartilage samples were digested in pronase (2 mg/mL, Roche, Basel, Switzerland) and collagenase D (1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM DTT supplemented with 1×cOmplete EDTA-free protease inhibitor cocktail (Roche), 1×PhosSTOP phosphatase inhibitor (Roche) ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... 2% b-mercaptoethanol) and cOmpleteTM mini EDTA-free protease inhibitor cocktail (Roche, #04693159001) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and whole-transcription amplification (WTA) with KAPA HotStart HIFI 2 × ReadyMix (Kapa Biosystems) for 20 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM NaF and 2 mM Na3VO4) containing a protease inhibitor cocktail (Roche). The cell lysates were clarified by centrifugation at 13,000 rpm for 15 min at 4 °C and then denatured with SDS-PAGE sample buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM PMSF and 1X cOmplete® EDTA-free protease inhibitor cocktail (Roche). The extracts were placed on ice for 15 minutes and then centrifuged 2 times at maximum speed in a benchtop centrifuge at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mg ml−1 iodoacetamide supplemented with Complete Protease Inhibitor Cocktail tablets (Roche) for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mg ml−1 iodoacetamide supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche) in Dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... cell nuclei were counterstained using 40,60-diamidino-2-phenylindol (DAPI, Roche, Mannheim, Germany). Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech ...
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bioRxiv - Immunology 2024Quote: ... minced and digested for 30 min in collagenase D (2 mg/mL, Roche) and DNase I (100 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded human intestinal tissues was carried out on a Discovery Ultra automated slide stainer using a rabbit anti-human monoclonal antibody for CEA (Clone T84.66, Roche Glycart AG, Switzerland) at 2.23 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Microbiology 2020Quote: ... and 3-4 mice/group subcutaneously received 25ng/g of pegylated-human interferon α (Peg-hIFNα-2a) (Hoffmann La Roche, Basel, Switzerland). To activate IFN-1 signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... Wing discs of L3 larvae were hybridized with probes overnight at 56 °C using standard procedures and visualized using anti-Dig-AP (1:1,000; Roche). Primers used for generating PCR templates are listed in Key Resources Table.
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bioRxiv - Genetics 2021Quote: ... Then they were incubated overnight at 4°C with primary antibodies diluted in a dilution buffer (0.5% blocking reagent (Roche), 2% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Epidermal and dermal sheets were prepared after incubation overnight at 4°C with 4 U/ml dispase II (Roche) in PBS by gently separating dermis and epidermis each as intact sheets (Rahn et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense DigoxigeninUTP-labeled RNA probes were synthesized at 37°C using RNA DIG labeling mix per manufacturer’s instructions (Roche) using RNA polymerase ...
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bioRxiv - Developmental Biology 2021Quote: ... Skin was cut into 1mm sized pieces and digested for 45 minutes at 37°C by 0.25 mg of Liberase DL (Roche) per mL of EHAA media and DNAse (Worthington) ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment for 1 hour at 37°C and cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602). PCR reactions were conducted as follow ...
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bioRxiv - Developmental Biology 2022Quote: ... fins were incubated for 20 min at 28°C with vigorous shaking in a solution of Liberase DH Research Grade (0,05mg/ml in 1x PBS, Roche). Cell suspensions were passed through a 30 μm filter (CellTricks ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with cold PBS and lysed for 20 minutes at 4°C in lysis buffer (10mM Tris pH 8, 10mM NaCl, 0.2% NP-40, supplemented with cOmplete proteinase inhibitors (Roche)) prior to snap freezing in 1ml lysis buffer on dry ice ...
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bioRxiv - Epidemiology 2019Quote: ... and C-reactive protein (CRP) was measured by automated particle-enhanced immunoturbidimetric assay (Roche UK, Welwyn Garden City, UK). CRP levels were measured at nine ...
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bioRxiv - Immunology 2021Quote: Mouse tracheal epithelial cells were isolated from tracheas digested overnight at 4 °C in Ham’s F12 medium plus pronase (1 mg/ml; Roche). Cells were cultured for 3 h on Primaria plates (Falcon ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants from each buffer were dialyzed in 25 mM Tris-HCl and 5 mM EDTA pH 8.0 overnight at 4°C and subsequently digested with 200 mg/ml pronase (Roche). Peptides were precipitated with 5% trichloroacetic acid (TCA ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were harvested 4 hours after induction at 37°C and lysed by sonication in 1x PBS pH 7.5 with 1 tablet protease inhibitor cocktail (Roche). After cell lysis and centrifugation at 30,000g ...
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bioRxiv - Cancer Biology 2020Quote: Tumors were mechanically dissociated and digested for 45 min at 37 °C in RPMI 1640 with 37.5 µg/mL Liberase TM (Roche) and 8,000 U/mL DNase I ...
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bioRxiv - Cell Biology 2021Quote: ... then 10-15 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) containing 0.4 mg/ml Protease (Roche) and then with 15-20 ml of 37 °C EBS containing collagenase D (0.1 U/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 12-20 ml of pre-warmed to 37 °C enzyme buffer solution (EBS) with 2.3 U of Liberase Blendzyme 3 recombinant collagenase (Roche) were cannulated into the vena cava to isolate hepatocytes ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Plant Biology 2020Quote: ... roots were incubated overnight at 4°C in anti-myc-mouse primary antibody (1:250, Roche in blocking solution), washed 3 times in PBST ...
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bioRxiv - Microbiology 2021Quote: ... C-reactive protein and D-dimer levels were measured using Cobas Integra 400 plus analyzer (Roche Diagnostics Nederland B.V.).
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bioRxiv - Immunology 2021Quote: ... ankle joints were teased apart and shaken at 110 rpm at 37°C for 25 minutes with 2.68mg/mL collagenase D (Roche) in RPMI 1640 (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were immunoblotted for 1 hr at 22°C using the following antibodies: rat anti-HA (1:3000; Roche), mouse anti-V5 (1:5000 ...