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Citations for Roche :
951 - 1000 of 2072 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 2× concentration of protease inhibitor cocktail (cOmplete, EDTA free #05056489001, Roche), 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 20 U/ml of recombinant huIL-2 (Roche, 11147528001) and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... and treated for 2 hours with 10U/ml DNase-I (Roche) before being concentrated by ultracentrifugation through a 20% sucrose/PBS cushion (28,000 rpm on a Sorvall Surespin rotor ...
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bioRxiv - Developmental Biology 2020Quote: ... and 2% SDS) with proteinase K (0.17 mg/mL, Roche Diagnostics) and inactivated 10 min at 95°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% NP-40) and 2 µl protease K (Roche, Cat 10910000) for at least 3hrs at 55℃ ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2% SDS) with proteinase K (0.17 mg/mL, Roche Diagnostics) and inactivated 10 min at 95°C ...
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bioRxiv - Genetics 2020Quote: ... 2 mM DTT and complete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM ATP) using one Protease Inhibitor Cocktail Tablet (Roche) per 100 mL of the lysate ...
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bioRxiv - Cell Biology 2022Quote: ... or with 1:10 Dnase I (stock 2 U/μL, Roche), and micrococcal nuclease (stock 2000 U/μL ...
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bioRxiv - Immunology 2022Quote: ... 2 ml of DMEM with 0.26 U ml−1 LiberaseTM (Roche) and 0.25 mg ml−1 DNase I (Roche ...
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bioRxiv - Immunology 2022Quote: Cells in cRPMI-10 with 20 U/ml IL-2 (Roche) were activated with Cell Stimulation Cocktail (Affymetrix eBioscience ...
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bioRxiv - Genomics 2022Quote: ... transposed fragments were amplified using 2×KAPA HiFi HotStart ReadyMix (Roche) and Nextera DNA CD Indexes (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM β-mercaptoethanol) supplemented with protease inhibitor cocktail (#11873580001, Roche) and 200 unit/ml RNAsin (#N2515 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 2 µL of 20 mg/mL Proteinase K (#3115879001, Roche) was added and again incubated at 55°C for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of 10X 5-Bromo-2’-deoxyuridine (BrdU) (Roche, Germany) per well was added ...
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bioRxiv - Neuroscience 2023Quote: ... 2% NaF) ) supplemented with EDTA-free protease inhibitors (cOmplete™, Roche) for 30 min ...
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bioRxiv - Genomics 2024Quote: ... with SYBR Green 2× PCR Master Mix I (Roche, Cat# 04887352001) and 1 μM of forward and reverse primer respectively ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2% SDS) with proteinase K (0.17 mg/mL, Roche Diagnostics) and inactivated 10 min at 95 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM MgCl2) supplemented with 0.3 mg/mL DNAse I (Roche), 1 tablet of phosphatase inhibitor (PhosSTOP ...
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bioRxiv - Microbiology 2023Quote: ... and 2 U of Expand High Fidelity Taq DNA polymerase (Roche) and 40 PCR cycles ...
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bioRxiv - Evolutionary Biology 2022Quote: 2 μl 1x proteinase K (recombinant PCR Grade, 25 mg - Roche) dissolved in 2,5 ml TE (10 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... or 1µg/mL 4’,6’-diamidine-2’-phenylindole dihydrochloride (DAPI) (Roche).
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Microbiology 2023Quote: ... 2% glycerol (v/v)] supplemented with complete protease inhibitor cocktail (Roche). Cell lysates were scraped into a pre-chilled micro-centrifuge tube and incubated with agitation for 30 min at 4°C followed by sonication at 40% intensity three times in 10 second intervals ...
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bioRxiv - Developmental Biology 2023Quote: ... and 2 ng/ml basic fibroblast growth factor (Roche Applied Science). Experiments were performed at passages 6-8.
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bioRxiv - Immunology 2023Quote: ... 2 mM sodium vanadate) supplemented with cOmplete protease inhibitor cocktail (Roche), 100 μM PMSF ...
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bioRxiv - Systems Biology 2024Quote: We amplified the cDNA using 2× Kapa HiFi HotStart ReadyMix (Roche), a high-fidelity polymerase enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... consisting of 2 μL 5X KAPA HiFi HotStart Buffer (Roche, KK2502), 0.048 μL of 10 mM dNTPs ...
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bioRxiv - Immunology 2023Quote: ... samples were counterstained with 4’,6-Diamidino-2-phenylindol (DAPI, Roche).
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bioRxiv - Biochemistry 2024Quote: ... 2 μg ml-1 pepstatin and protease inhibitor cocktail tablets (Roche). The cells were then lysed by sonication and cell debris was removed by centrifugation at 30,000 g for 45 min at 4° C ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μg ml-1 pepstatin and protease inhibitor cocktail tablets (Roche) and lysed with an EmulsiFlex-C3 homogenizer at pressures above 20,000 psi ...
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bioRxiv - Immunology 2023Quote: ... the media was supplemented with IL-2 [10 lU/mL] (Roche) and IL-15 [2 ng/ml] (BioLegend) ...
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bioRxiv - Cell Biology 2023Quote: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Roche) was performed following manufacturer’s indications ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... with 2 µL of the Fluorescein labeling mix (Roche, Basel, Switzerland), instead of the recommended 2 µL of each NTP ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 mM DTT) supplemented with complete protease Inhibitor cocktail (Roche) and 0.1 mg/ml cycloheximide or no cycloheximide ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1% Tween 2) followed by incubations in MABT blocking solution (Roche) and anti-DIG-AP Fab fragments (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mg/ml lysozyme] with 1X protease inhibitor cocktail (Roche, USA) for 1 hour at 4 °C and subjected to sonication (30 sec pulse with 50 sec intervals for 10 mins at 40% power on ice ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in DAPI (2 µg/ml, 10236276001, Roche Merck) in PBS (10 min) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 U/mL bovine DNase I (Roche, Switzerland, Cat. No. 11284932001), 25 mM MgCl2 ...
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bioRxiv - Genomics 2019Quote: ... using the DNA 1000 kit and quantified by qPCR using the KAPA Library quantification kit (Roche, ref. KK4824). The library was sequenced on one lane of Hiseq2500 in single read 100nt mode using the clustering and SBS v3 kit following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated in PEMBALG containing monoclonal anti-HA antibody produced in rat (1:1000 dilution, Roche) for one hour ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study and their respective dilutions were: mouse monoclonal anti-GFP at 1/1000 (clones 7.1 and 13.1, Roche), mouse monoclonal anti-TY at 1/200 (53) ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study and their respective dilutions were mouse monoclonal anti-GFP at 1/1000 (clones 7.1 and 13.1, Roche), mouse monoclonal anti-TY at 1/250 (53) ...
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bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase (AP) staining was performed using anti-DIG-AP antibody (diluted at 1:1000, 11093274910; Roche Diagnostics) and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT ...
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bioRxiv - Microbiology 2024Quote: ... The remaining 90% of each sample was immunoprecipitated with 1:1000 anti-HA antibody (0.5 mg/mL Roche Rat Anti-HA High Affinity [11867423001] ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies and reagents were used in this study: rat anti-HA (Roche, 3F10, 1/1000), rabbit anti-GFP (Invitrogen ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... 2 mM benzamidine and cOmplete protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany)) and incubated with zymolyase 20T for ∼20min at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lumbar vertebrae 1 – 5 were incubated in 2% Collagenase P (Roche, Switzerland) for 30 minutes at 30° C ...