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Citations for Roche :
951 - 1000 of 1191 citations for Dengue Virus Serotype 3 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ∼ 200 embryos of 1dpf were placed in 1mL modified Ringer’s solution (116 mM NaCl, 3 mM KCl, 4 mM CaCl2, 5 mM HEPES; pH 7.5) containing Protease Inhibitor (Roche, Cat. No. 04693132001) and Phosstop (Roche ...
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bioRxiv - Immunology 2024Quote: ... tissue sections (n = 2-3 per tissue type) underwent deparaffinization and heat-mediated antigen retrieval on the Ventana Discovery Ultra auto-stainer platform (Roche Diagnostics, Canada), following the below instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Reaction volumes were increased to 50 ul and 3 μl was subsequently used for qPCR reactions with SYBR Green (Roche, cat# 04887352001). Forward primers were designed at the 5’ ending of tRNAs and the reverse primers were designed against the 3’adapter while specificity was created against the 3’ endings ...
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bioRxiv - Cell Biology 2020Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2019Quote: ... Japan) was employed for detection of proteins visualized by Lumi-light Plus Western blotting substrate (Roche, Basel, Switzerland).
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bioRxiv - Developmental Biology 2019Quote: ... Supernatant containing cytosol protein was mixed with 1% SDS solution supplemented with proteinase inhibitor (PI) cocktail (Roche, 5892970001), and then was used for western blotting ...
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bioRxiv - Immunology 2019Quote: ... The protein was produced by transient transfection of HEK 293T cells with XtremeGene™ HP Transfection reagent (Roche) according to the manufacturer’s instructions and purified following a published protocol (77) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to PVDF membranes and labelled overnight with anti-HA (0.01 μg/mL, Roche clone 3F10), anti-VE-Cadherin (0.5 μg.ml−1 ...
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bioRxiv - Biochemistry 2019Quote: Overall protein was extracted through RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China) with protease inhibitors (Roche, Basel, Switzerland), and then quantified through BCA™ Protein Assay Kit (Pierce ...
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bioRxiv - Molecular Biology 2019Quote: ... and were then stained with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA) and the FITC-tagged secondary antibody ...
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Individual differences in honey bee behavior enabled by plasticity in brain gene regulatory networksbioRxiv - Genomics 2020Quote: ... with protein inhibitor complex (PIC, Complete Tablets, EDTA-free Protease Inhibitor Cocktail from Roche, Basel, Switzerland, cat. #04693132001) using a motorized pestle for 20 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent amounts of protein samples were separated by SDS-PAGE and electroblotted onto a polyvinylidene fluoride membrane (Roche) using a Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... shTDP-43 HEK293E cell pellets from confluent 10cm plates were washed twice in ice-cold PBS and then resuspended in 500μl of hypotonic buffer A (10mM HEPES, 1.5mM MgCl2, 10mM KCl, 0.1mM DTT and protein inhibitor cocktail (Roche) and incubated on ice for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 spike protein monoplex IHC was conducted using a Chromomap DAB IHC kit (Roche, Basel, Switzerland) with CC1 antigen retrieval at 95°C for 64 minutes ...
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bioRxiv - Microbiology 2020Quote: Proteins from various cells were extracted with 1x RIPA buffer containing 1x complete-mini protease inhibitor (Roche; #11836170001) and 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2020Quote: Total proteins were extracted from mouse striata in PBS containing 0.1% Tween 20 and Protease Inhibitor cocktail (Roche) with or without 1 mM non-hydrolysable ADP (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of the proteins of interest was tested by immunodetection using rat anti-HA-peroxidase (3F10, Roche) or chicken anti-c-Myc primary antibody (A2128 ...
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bioRxiv - Cancer Biology 2019Quote: All A673/TR/shEF in vitro and xenograft proteins were extracted with RIPA and anti-protease cocktail (Roche). Western blots were hybridized with rabbit monoclonal anti-FLI1 antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The TM protein was produced by transient transfection of HEK 293T cells with XtremeGene HP Transfection reagent (Roche) according to the manufacturer’s instructions and purified following a published protocol (48) ...
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bioRxiv - Immunology 2019Quote: ... These cells were then lysed in T-PER tissue protein extraction reagent (Pierce) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1(Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Protein was purified from filtered cell supernatants using StrepTactin resin (IBA) or cOmplete His-Tag Purification Resin (Roche) or Jacalin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: After being pre-cleared with protein A/G-coupled Sepharose beads (Cat. 11134515001 and 11243233001, Roche, Mannheim, Germany) for 2 hrs ...
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bioRxiv - Microbiology 2020Quote: Protein was extracted from cells in Radioimmunoprecipitation assay (RIPA) lysis buffer containing 1X Complete Protease Inhibitor Cocktail (Roche) and 1X Phenylmethylsulfonyl fluoride (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Separated proteins were transferred to PVDF membranes (GE Water & Process Technologies) and treated with Western blocking reagent (Roche) for overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The protein was produced by transient transfection of HEK 293T cells with X-tremeGENE HP Transfection Reagent (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal antibodies were used to detect green fluorescent protein (GFP) (Cat#11814460001, clones 7.1 and 13.1, Roche). Rabbit polyclonal anti-Cdc42p antibodies were provided by Dr ...
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bioRxiv - Immunology 2022Quote: Protein extraction was performed using RIPA buffer in the presence of complete Mini EDTA-free protease inhibitor (Roche) and PhosSTOPTM phosphatase inhibitor (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Equal quantities of protein were electrophoresed on 10% SDS-PAGE and transferred onto a nitrocellulose membrane (Roche Diagnostics). The membranes were incubated with the primary antibody overnight at 4°C after blocking with 5% milk dissolved in TBST buffer for another 2 h at room temperature (25°C) ...
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bioRxiv - Molecular Biology 2022Quote: ... the frozen brain samples were homogenized in tissue protein extraction reagent (Pierce) supplemented with complete protease inhibitors (Roche), and centrifuged for 1 hour at 430 000 g at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... The enriched FLC-Venus protein was detected by western blot assay with the antibody anti-GFP (11814460001, Roche). Signals were visualized with chemiluminescence (34095 ...
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bioRxiv - Pathology 2024Quote: Cholesterol and triglyceride levels were quantified in liver protein lysates and EDTA-plasma using enzymatic colorimetric assays (c.f.a.s. cobas, Roche Diagnostics) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted in RIPA buffer (Cat. #89901, Pierce) containing complete protease inhibitor cocktails (Cat. #04693116001, Roche) and phosSTOP (Cat ...
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: For immunoblot analysis of proteins, tissues were homogenized in RIPA buffer (R0278, Thermo) with proteinase inhibitors (11836170001, Roche) and phosphatase inhibitors (04906837001 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were pre-cleared by incubating with 30 μl of protein G-agarose beads suspension (Roche, cat. # 11719416001) for 3 h at 4°C on a mini-rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... Two days after transfection cells were washed twice with ice-cold PBS and scraped in ice-cold Co-IP buffer (10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, protein inhibitor cocktail (Roche)) supplemented with 0.5% Nonident P40 ...
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bioRxiv - Cancer Biology 2023Quote: ... The His-tagged-TRF2TRFH protein was purified in presence of cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) by using Protino ® Ni-TED 2000 Packed Columns (Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... HA- and GFP-tagged proteins were detected using horseradish peroxidase-conjugated monoclonal anti-HA (Roche, 3F10, 1:5,000) and anti-GFP (Roche ...
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bioRxiv - Cell Biology 2024Quote: Cell protein was extracted on ice in cold whole-cell RIPA buffer supplemented with protease inhibitor cocktail (Roche). SDS-PAGE was performed using 10% Tris-glycine gels ...
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bioRxiv - Cancer Biology 2024Quote: Cell lines: Cells were lysed with RIPA buffer and prepared for total protein extraction with protease inhibitors (Roche). After 20 minutes of centrifugation (>16000g) ...
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bioRxiv - Cancer Biology 2024Quote: ... protein samples were subjected to 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Roche, Mannheim, Germany). Afterwards ...
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bioRxiv - Pathology 2024Quote: ... Pre-cleared lysates (containing 1 mg of protein) were then incubated with mouse monoclonal anti-GFP antibodies (Roche) coupled to G-protein beads for 3 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Sections from adra1A KO mice were rinsed with PBS (3 × 5 min) and incubated in a β-gal staining solution (Roche, Ref # 11828673001) overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Enrichment of 2 μg of an indexed library incubated with 3 μM of a pool of biotinylated oligonucleotides was performed using the SeqCap EZ Hybridization reagent kit (Roche/NimbleGen, Lot # 05634261001), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-bromo-4-chloro-3-indolylphosphate (BCIP) and nitroblue tetrazolium chloride (NBT) according to the manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). The sections were then mounted ...
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bioRxiv - Cell Biology 2019Quote: ... 5 μg/ml taxol, 3 U/ml DNAse I, 10 μg/ml RNAse A, 1 U/ml micrococcal nuclease, and Roche Complete Protease Inhibitors) and vortexed vigorously for 1 min ...
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bioRxiv - Developmental Biology 2020Quote: ... for 16-20h incubation at room temperature and colorimetric signals were detected using Nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphase (NBT-BCIP; Roche Applied Sciences 11383221001). RNAScope ISH was conducted for FGF15 and Ptch1 ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgOAc, 5 %w/v glycerol, 0.25 %w/v NP-40, 3 mM DTT, 1 mM PMSF, Roche protease inhibitor cocktail (PIC), pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... 80 µl of dephosphorylation solution (8 μl 10x dephosphorylation buffer, 2 μl RNasin Plus, 67 μl ddH2O, and 3 μl alkaline phosphatase (Roche, catalog no. 10713023001)) was added to the beads ...