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Citations for Roche :
51 - 100 of 2929 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... each fatty acid was first conjugated to 10% fatty-acid-free BSA (bovine serum albumin fraction V) (#10735086001, Roche) for 1 hour at 50°C in a 50:50 volumetric ratio ...
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bioRxiv - Immunology 2021Quote: ... All primary and secondary antibodies were diluted in 5% (v/v) western blotting reagent (Roche, Basel, Switzerland). After washing for 3 times in TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The following primary antibodies were used: GCase (5 µg/mL; hGCase-1/23; mouse monoclonal; Roche [62]); LAMP1 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were lysed with the Lysis Binding Buffer from the Magnapure LC Kit 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from 253 resuspended vaginal swab samples using the MagNA Pure Compact Nucleic Acid Isolation Kit (Roche) [33] ...
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bioRxiv - Immunology 2019Quote: ... Fatty acid free (FAF) BSA was from Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% deoxycholic acid) with protease inhibitor mixture (Roche) and clarified by centrifugation at high speed (20,000 rcf) ...
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bioRxiv - Physiology 2021Quote: ... 0,5% deoxycholic acid and protease inhibitors (11697498001, Roche). Cells were then scrapped ...
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bioRxiv - Microbiology 2021Quote: ... and ADNaCl with fatty-acid-free BSA (Roche). Modified Sauton’s solid medium contained 1.5% bactoagar (BD ...
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bioRxiv - Microbiology 2023Quote: ... and ADNaCl with fatty-acid-free BSA (Roche). For modified Sauton’s solid medium ...
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bioRxiv - Microbiology 2023Quote: ... 0.4% blocking reagent for nucleic acids (Roche, Switzerland)) ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.1% deoxycholic acid with protease inhibitors (Roche). The lysates were subjected to anti-GFP immunoprecipitation using GFP- Trap beads (ChromoTek) ...
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bioRxiv - Microbiology 2023Quote: ... or High Pure Viral Nucleic Acid kit (Roche) kits ...
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bioRxiv - Molecular Biology 2024Quote: ... Ethylenediamine tetraacetic acid -free protease inhibitor cocktail (Roche) was added fresh to the binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... samples were enzymatically analyzed using a D-Lactic Acid/L-Lactic Acid Enzymatic Bioanalysis UV-Test kit and a D-Glucose Enzymatic Bioanalysis UV-Test kit (Roche Diagnostics) with a modified manufacturer’s protocol to reduce total sample size to 300 uL ...
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bioRxiv - Microbiology 2019Quote: Viral nucleic acid was extracted from 200µl of the filtrate using the High Pure viral nucleic acid kit (Roche Diagnostics, USA) following the standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then blocked for an hour in 5% milk powder/PBS solution and probed with 1:2,500 rat anti-HA mAb 3F10 antibody (Roche) in 5% milk powder/PBS solution overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... for nucleic acid hybridization and detection (BBR, Roche, 11096176001) in MAB buffer at RT for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... using SYBR green as nucleic acid stain (Roche 4913850001). To specifically quantify mRNA expression ...
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bioRxiv - Molecular Biology 2020Quote: ... were diluted 10 times in binding buffer (20mM Tris pH 8.0, 100mM NaCl, 5mM MgCl2, 0.4% NP40) and supplemented with protease inhibitors (Roche), 60µg of yeast tRNA (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Real time PCR was performed with SYBR green as the DNA binding dye (Roche Applied Science, Mannheim, Germany) on a 7900HT Fast Real-Time PCR System (Applied Biosystems under Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Membranes was incubated secondary antibody for an hour in 5% milk and developed using Lumi-light western blot substrate (Roche) to detect HRP ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
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bioRxiv - Genomics 2020Quote: ... They were then incubated with TNB (5% FBS in TNT) for 15 min before incubation with peroxidase-conjugated anti-DIG antibody (DIG-POD, Roche) 1:200 in TNB for 2 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... a linear DNA tether of the same length was amplified using a digoxigenin-modified primer instead of NTA (5’-DIG-NHS-TCCAAAGGTGAAGAACTGTTCACC, Integrated DNA Technologies, Inc. USA) to bind to anti-digoxigenin Fab fragment antibodies (11214667001, Roche).
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bioRxiv - Microbiology 2022Quote: ... Hybridised probes were detected with anti-DIG antibodies and revealed with alkaline phosphatase-conjugated antibodies in presence of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP, Roche).
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bioRxiv - Plant Biology 2023Quote: ... membranes were blocked with 5% skim milk and then incubated in the primary anti-GFP mouse monoclonal antibody (Roche, 11814460001), followed by goat anti-mouse HRP conjugate (BioRad ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were incubated with blocking solution (5% skimmed milk in 1X PBS) followed by overnight incubation with respective primary antibodies (anti HA rat 1:1000 (Roche), anti BiP rabbit 1:5000 in cold room and 1 hr incubation with respective secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from 400 µl of cerebrospinal fluid using the MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Diagnostics, Indianapolis, IN, USA) on the MagNA Pure compact automated extractor ...
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bioRxiv - Biochemistry 2021Quote: ... 5’ RACE was performed with the 5’ RACE kit (Roche) using gene-specific reverse primers ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl of 5 x KAPA HiFi buffer (Roche, #KK2101) and 16.75 µl H2O ...
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bioRxiv - Developmental Biology 2019Quote: ... and detected with the DIG Nucleic Acid Detection Kit (Roche). Wing imaginal discs were mounted in glycerol and imaged with a Nikon E200 bright-field microscope.
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bioRxiv - Molecular Biology 2019Quote: ... Nucleic acids were removed with 250 U of Benzonase (Roche) for 1 hr at 4° C ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mM ascorbic acid) containing a protease inhibitor cocktail (Roche). All subsequent steps were conducted on ice ...
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bioRxiv - Plant Biology 2022Quote: ... and ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail cOmplete (Roche). For blocking and antibody dilutions ...
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bioRxiv - Neuroscience 2021Quote: ... Okadaic acid (200nM) and a protease inhibitor cocktail (Roche Complete) with a Dounce homogenizer and solubilized for 1 hour rotating at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 0.25% sodium deoxycholic acid and complete protease inhibitor cocktail (Roche), pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were dissected in ice cold 1x PBS and transferred to low binding PCR tubes containing 50-100µL of 1x Protease Inhibitor Cocktail in 1xPBS (cOmplete mini EDTA-free; Roche) on ice ...
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bioRxiv - Genomics 2020Quote: ... and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were lysed (Avestin Emulsiflex C5) in lysis/binding buffer (20mM phosphate [pH7.4], 500mM NaCl, 20mM imidazole, protease inhibitor cocktail [Roche]), clarified lysate was applied to a 5mL HisTrapHP column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... beads were washed with150 μL cold BSA/PBS for 3 times and mixed with 100 μL Binding Buffer (1% Triton X-100, 0.1% Sodium Deoxycholate, 1x complete protease inhibitor (Roche)) plus 100 μL 0.2 μg/μl chromatin followed by overnight incubation on a rotating platform at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Hi5 cells were harvested by centrifugation and resuspended in binding buffer (50 mM Tris, 300 mM NaCl, pH 8.0) with 1 mM PMSF and protease inhibitors (Complete, Roche). The cells were lysed by sonication and pelleted for 20 min at 21,000g using a 4°C Eppendorf 5424 centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in binding buffer (50 mM Tris pH 8.0, 10 mM imidazole, 500 mM NaCl) supplemented with cOmplete protease inhibitors (Roche), and sonicated ...
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bioRxiv - Molecular Biology 2024Quote: Ni-NTA Magnetic Agarose Beads were incubated with 1.5µg of recombinant His-TIRR(8) in binding buffer (50mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 10% Glycerol, Protease inhibitors (EDTA free, Roche)) for 1 hour at 4°C on a wheel ...
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bioRxiv - Bioengineering 2020Quote: ... ITS (5 μg/mL insulin, 5 μg/mL transferrin and 5 ng/mL sodium selenite; Roche Diagnostics GmbH), and 5 % fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked for at least 1 hour in blocking solution at RT (5% milk in 1xTBS) before probing with primary antibody in blocking solution (α-HA-HRP, 1:2500 (Roche); α-PGB1 ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with primary antibodies (5% milk, TBS-T, overnight, 4°C) at the following dilutions: rat anti-HA (1:1000; Roche Diagnostics), mouse anti-Myc (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 50 µl of the post-nuclear supernatant was saved as the input lysate and 450 µl was incubated with 5 µg of anti-GFP antibody (Roche 11814460001) for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: ... incubated over-night (o/n) at 4°C with primary antibody diluted 1:500 in 5% Bovine Serum Albumin Fraction V (Roche 10735086001) in TBS-T ...