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Citations for Roche :
51 - 91 of 91 citations for Fmoc Rink Amide OctaGel Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The lysate supernatant was mixed with 200 µL of Ni-nitrilotriacetic acid resin (Roche Complete His-Tag purification resin ...
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bioRxiv - Immunology 2023Quote: ... Secreted IgGs were purified from cell supernatants with Protein A resin (Roche, Basel, Switzerland). IgGs were eluted at low pH using Protein A elution buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of a slurry of cOmplete His-Tag Purification Resin (Roche Molecular Diagnostics) was equilibrated with lysis buffer (50 mM NaH2PO4 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were purified by affinity on cOmplete™ His-Tag Purification Resin (Roche®). Columns were washed with Buffer A followed by washing with 50 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant incubated with 1 ml Complete His-Tag Purification Resin slurry (Roche) and Complete EDTA-free protease inhibitor cocktail tablet (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Then concentrated supernatant was purified by immobilized metal-affinity chromatography with Ni-NTA resin (Roche) stocked in WET FRED gravity flow columns (IBA ...
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bioRxiv - Cell Biology 2024Quote: ... supernatants were harvested and purified using His-Tag purification resin (Roche, cOmpelteTM, Cat No.5893682001), followed by size-exclusion chromatography in 20 M Hepes or Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3h at 4°C ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The supernatants were then incubated with the Complete His-Tag Purification Resin (Roche, Basel, Switzerland) that had been prewashed with Renz buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were incubated with 10 mL of cOmplete His-Tag purification resin (Roche, Germany) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The protein eluted after immobilized metal affinity chromatography (IMAC) using complete His-tag purification resins (Roche) was concentrated using 10KDa cut-off amicon filters (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was incubated with 2 mL of pre-equilibrated Protein C affinity resin (Roche) for 3 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 5 mM imidazole and 500 μL cOmplete Hig-tag purification Resin (obtained from Roche, ref 05893682001). The mixture was gently shaken at 4°C for 4 h in dark ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysate was added to 1 mL cOmplete His-Tag Purification Resin (COHISR-RO, Roche) that had been pre-washed with buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the supernatant was further incubated with 25 μl of cOmplete His-tag purification resins (Roche, Switzerland) at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche) for 2h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the resin was washed with 20 mL HEGX buffer supplemented with protease inhibitor (1X, Roche #64178100), and then the protein eluted with elution buffer (HEGX buffer with 100 mM DTT [Merck # 1.24511.0005]) ...
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bioRxiv - Biochemistry 2021Quote: ... the presynaptic filament was formed by incubating 5 μl of streptavidin-coated magnetic resin (Roche Molecular Biochemicals) with 5’-biotinylated 80-mer ssDNA oligonucleotide (5 μM ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... HA:SQSopsin was immunoprecipitated from the detergent-soluble fraction using 70 μl of 50% protein G resin (Roche) slurry and anti-HA antibody (12CA5) ...
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bioRxiv - Biochemistry 2024Quote: ... 100 µL of a 50 % slurry of Ni-NTA resin (Roche; pre-equilibrated into low salt buffer) was added to the reaction and incubated for 30 min at 4 °C to capture His-Trx-BirA ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C) and the resulting supernatant was loaded onto 1 mL of cOmplete Ni purification resin (Roche). The resin was washed three times in 10 mL of lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Cell lysates were loaded onto a nickel immobilized metal affinity chromatography column (cOmplete His-Tag Purification Resin, Roche) and eluted with 250 mM imidazole ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 8G8-Fab were expressed with His-tag and purified using cOmplete His-Tag purification resin (Roche, USA) following standard protocols ...
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bioRxiv - Neuroscience 2024Quote: ... The protein was captured from the supernatant by affinity chromatography using cOmplete™ His-Tag Purification Resin (Roche), equilibrated with 50 mM sodium phosphate pH 7.8 ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified cell lysate was bound to 10 mL of cOmplete His-Tag purification resin (Roche, Germany, 30 min), washed with 1 L base buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was eluted with wash buffer containing 10 mM maltose and subsequently bound to c0mplete His tag resin (Roche) for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was then used in a batch purification protocol by addition of cOmplete His-Tag resin (Roche; 5893682001) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Microbiology 2023Quote: ... Expressed proteins in the cell-culture supernatant were purified using a cOmplete His-Tag Purification Resin (Roche, Cat# 5893682001) affinity column ...
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bioRxiv - Biophysics 2024Quote: The purification process began by purifying the His-tagged proteins through gravity open columns using cOmplete resin (Roche, USA), before subjecting them to concurrent dialysis and proteolysis ...
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bioRxiv - Molecular Biology 2021Quote: ... lysate supernatant from cells expressing N-terminal 6-His-tagged proteins were incubated with nickel-nitrilotriacetic acid (Ni-NTA) cOmplete His-tag purification resin (Roche) for 1 h at 4° C ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was loaded on a column filled with cOmplete His-tag Purification Resin (Roche Diagnostics GmbH, Mannheim, Germany) and pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... The His-GB1-tagged fusion TIRMyD88 was first purified with His-tag affinity column chromatography (cOmplete™ His-Tag Purification Resin: Roche). After removal of the His-GB1 tag with GST-HRV3C ...
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bioRxiv - Biophysics 2022Quote: ... and mixed with 1 mL Ni Sepharose 6Fast Flow (Cytiva, Tokyo Japan) (MinD) or cOmplete His-Tag Purification Resin (Roche, Basel, Switzerland) (MinE and msfGFP-MinC) ...
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bioRxiv - Cell Biology 2024Quote: ... Each reaction was then added to Strep-Tactin®XT 4Flow® high capacity resin (IBA # 2-5030-010) pre-equilibrated with XB buffer supplemented with cOmplete™ EDTA-free Protease Inhibitor tablets (Roche #11873580001) and/or PhosSTOP™ Phosphatase Inhibitor Tablets (Roche # 04906845001) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with load dilution buffer (solubilization buffer supplemented with 1% (w/v) sodium cholate) and loaded on a Ni-NTA affinity column (Roche cOmplete Ni-NTA resin) equilibrated with 10 CVs load dilution buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Tau variants were first purified via their N-terminal 6x-His tags by incubating the clarified lysate with cOmplete™ His-Tag Purification Resin (Roche, EDTA-and DTT-compatible) for 1 hour at 4 °C ...