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Citations for Roche :
901 - 950 of 1330 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: MYOrganoids proteins were extracted in RIPA buffer supplemented with Protease Inhibitor Cocktail EDTA-free (Roche) and Benzonase by homogenization ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were then electrotransferred onto a polyvinylidene fluoride (PVDF) membrane (Roche, Basel, Switzerland). Immunoblotting was conducted using a commercial HRP-conjugated Mouse anti GST-Tag antibody (Abclonal ...
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bioRxiv - Molecular Biology 2024Quote: Protein extraction was performed using RIPA buffer (Beyotime) supplemented with protease and phosphatase inhibitors (Roche). The protein concentration was determined using the BCA Protein Assay Kit (Epizyme Biomedical) ...
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bioRxiv - Immunology 2024Quote: ... Protein was then harvested after lysis in buffer containing a protease inhibitor (Sigma, 1183617001 Roche). Cell lysates were immunoblotted for β-actin and SCNN1γ ...
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bioRxiv - Plant Biology 2024Quote: ... The following antibodies were used for protein biochemistry experiments: αHA-Hrp (12013819001, Roche, 1/5000), αFlag-Hrp (A8592 ...
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bioRxiv - Molecular Biology 2024Quote: ... then mixed with 10 µL of protein G-agarose beads (PROTGA-RO, Roche, Basel, Switzerland) and incubated for 15 minutes on ice to capture proteins that non-specifically bind to the beads ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then treated with biotinylated Osteocalcin (Biotin Protein Labeling Kit, Cat# 11418165001, Roche, USA) with different concentration for 30 min at 37°C.
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bioRxiv - Cancer Biology 2024Quote: ... Protein - expression was detected with an OptiView DAB IHC Detection Kit (Roche Diagnostics, 760-700), optimized for automated immunohistochemistry (Benchmark GX stainer ...
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bioRxiv - Cell Biology 2024Quote: Immunoprecipitation (IP) of HA-tagged proteins was performed using Anti-HA Affinity matrix (1181501600, Roche) under denaturing conditions as described 16 ...
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bioRxiv - Developmental Biology 2024Quote: Proteins were collected in ice-cold IPLS lysis buffer including protease inhibitor cocktail (#11873580001, Roche) 48 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were used: anti-GFP mouse monoclonal at 1:250 (Roche, 11814460001), Alexa-647 conjugated HRP polyclonal at 1:500 (Jackson) ...
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bioRxiv - Microbiology 2020Quote: ... Infected mice were genotyped after experiments using the KAPA HotStart Mouse Genotyping Kit (KAPA Biosystems) and primers for the floxed IFNAR1 allele and the Lyve1-Cre gene from the Jackson Laboratory website.
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibodies used were anti-GFP from mouse (1:500 dilution, Roche, CAT# 11814460001) and anti-COXIV from rabbit (1:250 ...
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibodies used were anti-GFP from mouse (1:500 dilution, Roche, CAT# 11814460001) and an endogenous compartment marker antibody from rabbit (refer to Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Cell Biology 2022Quote: ... For analysis of TopBP1-YFP-AID mouse anti-GFP monoclonal (1:500, Cat#11814460001, Roche) and anti-mouse (IRDye® 800CW Goat anti-Mouse IgG ...
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bioRxiv - Microbiology 2020Quote: ... The isotype of antibodies was determined using the IsoStrip Mouse Monoclonal Antibody Isotyping Kit (Roche).
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bioRxiv - Cell Biology 2020Quote: The following primary antibodies were used: anti-GFP mouse monoclonal at 1:250 (Roche, 11814460001), anti-Cnn Rabbit polyclonal raised against first 660aa of Cnn-P1 (which includes amino acids 35-632 of Cnn-P2 ...
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bioRxiv - Cell Biology 2020Quote: ... The anti-GFP mouse monoclonal antibody mix (ref. 11814460001, 1:1000) was obtained from Roche. The rabbit anti-PKCζ (ref ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies used were: mouse monoclonal (mixture of clones 7.1 and 13.1) anti-GFP (Roche), 1:500 ...
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bioRxiv - Genetics 2021Quote: Samples of cDNA prepared from mouse tissues were analyzed either with a LightCycler instrument (Roche) with glass capillaries ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study include mouse αGFP (1:1000, 0.4 mg/ml, Roche), rabbit αGFP (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibodies used in this study were mouse αGFP (1:2000, 0.4 mg/ml Roche), rabbit αREX1-repeat (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse sterna and ribs from P3 neonates were cleaned and digested by pronase (Roche, 11459643001) at 2 mg/ml PBS at 37°C with constant agitation for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections (∼100 nm) were labelled using mouse anti-GFP antiserum (1:300, Roche 11814460001) and 18 nm Colloidal Gold AffiniPure Goat Anti-Mouse (Jackson ...
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bioRxiv - Cell Biology 2023Quote: ... washed and probed with antibodies against the GFP-epitope (dilution 1:2500, mouse, Roche 181446001), α-Tubulin (dilution 1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... All genotyping experiments were performed using the KAPA Hotstart Mouse Genotyping Kit (KAPA Biosystems, KK7352) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blotted with anti-GFP monoclonal primary antibody (mouse monoclonal, Sigma- Roche #11814460001) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... the nitrocellulose membrane was incubated with mouse anti-GFP antibodies (1∶1000, Roche Molecular Biochemicals) and rabbit anti-mKate2 antibodies (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from tail tissue using the KAPA Mouse Genotyping Kit (KAPA Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a mouse monoclonal anti-GFP antibody (Roche, 11 814 460 001, RRID:AB_390913), and anti-cdc2 (PSTAIR) ...
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bioRxiv - Genetics 2022Quote: ... Mouse exome libraries were constructed using the KAPA HyperPrep Kit (Roche Sequencing and Life Science) and SureSelect XT Community Design Mouse All Exon v2 Target Enrichment System (Agilent Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... and immunoprecipitated with 5 μg Mouse monoclonal anti-GFP antibody (clone 9E10, IgG, Roche, Switzerland). About 200∼300 bp of ChIP DNA and input DNA were recovered and dissolved in water for further qPCR analysis [82] ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse livers were perfused through the inferior vena cava with warm collagenase (Roche, Basel, Switzerland), and the portal vein was sectioned ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies used in this study: mouse monoclonal anti-GFP at 1:250 (Roche, 1814460), chicken polyclonal anti-GFP 1:40 (Abcam Ab13970) ...
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bioRxiv - Cell Biology 2020Quote: Protein was harvested in urea ammonium bicarbonate lysis buffer with complete EDTA free protease inhibitor (Roche) and phosphatase inhibitor (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with 60 µg of Protein A- or G-agarose bead slurry (Roche, Germany) for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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bioRxiv - Physiology 2021Quote: ... triglycerides and total protein were quantified using the Cobas c111 analyzer (Roche diagnostics GmbH, Mannheim Germany) as previously described by Segev-Hadar et al.38 ...
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bioRxiv - Molecular Biology 2022Quote: ... cell culture supernatants were harvested and proteins were purified from the supernatants using tandem Ni2+ (Roche) and Strep-Tactin (IBA ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were harvested in lysis buffer (RIPA buffer supplemented with protease inhibitor (Roche), sodium vanadate and sodium molybdate ...
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bioRxiv - Biochemistry 2020Quote: ... coli cell pellets were resuspended in protein buffer containing cOmplete protease inhibitor cocktail (Roche, Basel, Switzerland), lysed by sonication and centrifuged for 45 min at 38,000 g to remove cell debris ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were subsequently detected with HRP-conjugated anti-HA antibody (rat monoclonal, Roche, catalog number 12013819001) or mouse monoclonal anti-GFP antibody (Novus Biologicals ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein extraction was performed by adding 10 M urea containing complete protease inhibitor cocktail (Roche) and Halt™ Phosphatase Inhibitor (Thermo ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were incubated in presence of 20 µl of protein G agarose beads (Roche, 11243233001) for 3 h at 4°C on a rotor to reduce unspecific binding ...
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bioRxiv - Biophysics 2020Quote: ... The expressed protein is first purified by affinity chromatography using Ni-NTA coated agarose beads (Roche). His6-tagged proteins are eluted using 250 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche) at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized in a T-PER tissue protein extraction reagent supplemented with protease (Roche Applied Science) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... and 250 mg or 500mg protein was digested with 20 µg/ml proteinase K (PK) (Roche) for PrPSc blots for 1 hour at 37C ...