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Citations for Roche :
901 - 950 of 6235 citations for Adrenocorticotropic Hormone ACTH ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... followed by multiplex immunofluorescence staining with a U DISCOVERY 5 plex immunofluorescence (Roche Diagnostics, Meylan, France). Three sequential rounds of staining were performed each including heat deactivation step ...
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bioRxiv - Developmental Biology 2022Quote: ... rinsed 2 times for 5 minutes with alkaline phosphatase buffer and stained with NBT/BCIP (Roche) as described previously (Kraus et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 mM EDTA) in the presence of phosphatase and protease inhibitors (4906837001 and 4693159001, Roche). Cell lysate was subjected to immunoprecipitation with the related antibody and agarose beads ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM KCl) supplemented with protease and phosphatase inhibitor cocktails (Roche, cat#05892791001 and cat#04906837001), was used to homogenize brain tissue as described previously 55 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... At the desired treatment end-point 10 µl of 5 mg/ml MTT labelling Reagent (Roche) was applied per well and plates incubated at 37°C (5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 mM mgCl2 x 6 H2O and 5 mg/mL glucose-6-phosphate dehydrogenase (Roche Diagnostics) in 0.1 M potassium phosphate buffer pH 7.4 ...
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bioRxiv - Developmental Biology 2019Quote: ... 10% glycerol and 5% CHAPS) in the presence of protease inhibitors (cOmpleteTM Protease Inhibitor Cocktail, Roche). Immunoprecipitation of protein extracts was performed using a monoclonal anti-Flag antibody covalently attached to agarose (Anti-FLAG M2 Affinity gel ...
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bioRxiv - Molecular Biology 2020Quote: ... 9 µl of qPCR reaction mix containing 5 ul LightCycler 480 Probes Master (Roche, Cat#04707494001), 3.4 ul of Nuclease Free H2O (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5% v/v glycerol) supplemented with HP plus protease inhibitor mix (Serva) and DNase I (Roche). Cell lysates were prepared by sonication and cleared by centrifugation at 80000g at 4°C for 30-45 minutes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the medium was added with 10 μL of 5 mg/mL MTT solution (Roche, Basel, Switzerland), followed by adding the formazan diluted in about 200 μL of dimethylsulfoxide (Genview ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 mM EDTA) containing 1x protease inhibitor cocktail (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Roche). Cell debris was removed by centrifugation (8,000 g ...
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bioRxiv - Cell Biology 2019Quote: ... 5’UTRs and 3’UTRs amplifications were performed using either Expand High Fidelity PCR System (Roche) or KAPA HiFi PCR system (KAPA Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... the bill-skin preparation was treated for 5 minutes with 2 mg/mL collagenase P (Roche) in Krebs solution containing (in mM ...
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bioRxiv - Biophysics 2022Quote: ... 2 mM β-ME) supplemented with 5 μM pepstatin A and complete protease inhibitor tablets (Roche). All purification steps were carried out at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 5 mM imidazole and 500 μL cOmplete Hig-tag purification Resin (obtained from Roche, ref 05893682001). The mixture was gently shaken at 4°C for 4 h in dark ...
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bioRxiv - Molecular Biology 2023Quote: ... or pre-extracted for 5 minutes on ice with CSK/0.5% Triton buffer (supplemented with Roche Complete Protease inhibitor and PhosSTOP Phosphatase inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... After the membrane was blocked with 5% BSA (Bovine Serum Albumin Fraction V, Roche Diagnostics, Germany) in TBST buffer (1X Tris-Buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked for 1 hour at room temperature with 5% blocking agent (Roche Diagnostic) in TBST (100 rpm shaker) ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 mM EDTA) containing a protease inhibitor cocktail (cOmplete™, Mini Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and filtered prior to loading onto a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HBS ...
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bioRxiv - Microbiology 2023Quote: ... resuspendend in 10 ml of PBS buffer with 5 μg/ml DNaseI (from bovine pancreas, Roche) and 10 μg/ml lysostaphin and incubated for 20 min at 37°C to allow cell lysis ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... animals in PBST were rocked in 2% blocking buffer (5 g of blocking reagent, Roche, 11096176001) in 1X maleate buffer (150mM maleic acid ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2 μl (5 U/μl) FastStart™ Taq DNA Polymerase (Roche Diagnostics GmbH, Mannheim, Germany) in a total volume of 25 μl ...
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bioRxiv - Immunology 2023Quote: ... and equal amounts of protein from lysate were incubated with 5 μg anti-FLAG antibody (Roche) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... and 63 mM adenosine 5’-triphosphate disodium salt trihydrate brought to pH 7.0 (Roche: ATPD-RO), were stored at −20°C.
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bioRxiv - Cell Biology 2023Quote: ... Each RT-qPCR reaction (10 μl) contained 5 μl of SYBR Green Select Master Mix (Roche), 300 nM of the corresponding forward and reverse primers ...
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bioRxiv - Genomics 2023Quote: ... final extension 5 min at 62 °C) using the KAPA HiFi HotStart Ready Mix (Kapa Biosystems). Amplified libraries were purified ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5 supplemented with 5 mM dithiothreitol (DTT) and 1 x cOmplete protease inhibitor cocktail (Roche)) per gram of wet cell paste and incubated for 1 h at 4°C under stirring ...
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bioRxiv - Neuroscience 2023Quote: ... and nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrate (Roche Diagnostics). The color was allowed to develop for 5 hours ...
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bioRxiv - Immunology 2023Quote: ... ‘reverse’ 5’-GGGTACTTGATTTCATAGACTTTA-3’) were used in a qRT-PCR on a LightCycler 480 II (Roche). The data were analysed with LightCycler® 96 SW 1.1 (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT) substrate (Roche Diagnostics) for chromogenic detection ...
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bioRxiv - Cell Biology 2023Quote: ... 12.5% glycerol) containing 5 mM DTT and protease inhibitor cocktail tablets (PIs, cOmplete EDTA-free, Roche). Cells were lysed by sonication for 3 min (ON 3 sec ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM EDTA pH 8.0) with 1X complete protease inhibitors (cOmplete Protease Inhibitor Cocktail, Roche 11697498001) by vortexing ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μl of 10% SDS and 5 μl of 10 mg/ml Proteinase K (Roche, #31158) were added and incubated at 50 °C for 1 h ...
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bioRxiv - Physiology 2024Quote: ... with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrate (Roche Diagnostics). The color was allowed to develop for 1 hour (gal) ...
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bioRxiv - Physiology 2024Quote: ... and visualized using 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium substrate (Roche Diagnostics). After color development for 15 min (gal) ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 μL of 5 μM reverse primer and 10 μL of KAPA SYBR FAST (KAPA Biosystems). The qRT-PCR was done in CFX96 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM imidazole] containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease inhibitor cocktail (Complete™; Roche), and recombinant proteins were purified by immobilized metal affinity chromatography (TALON ...
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bioRxiv - Biophysics 2024Quote: ... 5% glycerol) per 1 L of cells with one EDTA-free protease inhibitor cocktail tablet (Roche). Cells were lysed using a Misonix Sonicator 3000 (110 W for 2 min total ON-time ...
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bioRxiv - Biochemistry 2024Quote: ... in 5 % BSA in room temperature for 1 hour followed by 2.5 ug/ml DAPI (Roche) for 20 minutes ...
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bioRxiv - Genomics 2024Quote: ... We reconstituted 5 mg of the enzyme Liberase™ (Thermolysin Medium) Research Grade (LIBTM-RO Roche) in 2 mL of PBS (to give a stock concentration of 2.5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed in 96-well plates on a Roche LC 480 or z480 light cycler (Roche, Basel, Switzerland) according to the CDC procedure including a previously negative patient specimen (extraction and RNaseP internal controls) ...
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bioRxiv - Biochemistry 2019Quote: ... and MePCE overexpression in MEF cells were grown on 10 cm plates to be harvested and lysed using a standard RIPA buffer mixed with cOmplete Protease inhibitor cocktail (Roche). MEFs were generated from Jmjd6tm1.1Gbf knockout mice as previously described (PMID ...
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bioRxiv - Plant Biology 2019Quote: ... PCR was performed using 96-well white PCR plates using the LightCycler® 480 II system (Roche Applied Science, Germany). The amplification started with an initial denaturation step at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Following the preparation of plates an incubation at 4°C for at least 60 min was performed before the plates were analyzed on a LightCycler 480 II (Roche) using 45 cycles and a temperature profile recommended by the manufacturer for miRCURY SYBR® Green mastermix ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were prepared as previously described (Plate et al., 2016) in RIPA buffer with fresh protease inhibitor cocktail (Roche). Total protein concentration in cellular lysates was normalized using the Bio-Rad protein assay ...
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bioRxiv - Biophysics 2020Quote: HeLa cells were plated in 6-well plates and infected with virus at equal amounts of p24 after treatment with DNaseI (Roche) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells in culture were seeded into 96 well plates and transfected in suspension using X-tremeGENE HP DNA Transfection Reagent (Roche). Transfection complexes were prepared such that each reaction contained 11 μL of Opti-MEM ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed using a 96-well plate in a pre-heated LightCycler® 480 Instrument II (Roche, Basel, Switzerland). Each well contained a 10 μl reaction mixture comprising 1 μl DNA template ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...