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Citations for Roche :
901 - 950 of 7679 citations for 7 Bromoacetyl 6 ethyl 1 2 3 4 tetrahydro 1 1 4 4 tetramethylnaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the mPFC tissues of CamK2A-Cre;Gpr158fl/fl mice and the controls (n = 4) at the age of 8 weeks with Tripure Isolation Reagent (Roche, Mannheim, Germany). RNA-sequencing (RNA-seq ...
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bioRxiv - Systems Biology 2023Quote: ... Then the pulverized samples were solubilized with 1000 μL extraction buffer (4% SDS, 100 mM Tris pH 8 supplemented with a cocktail of 1X protease inhibitors (Complete mini, Roche Diagnostics GmbH), 5 mM tris(2-carboxyethyl)phosphine (TCEP) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell pellets were lysed in 300 µL of lysis buffer (10 mL BugBuster® [Millipore 70584-4], ¼ tablet cOmplete™ Protease Inhibitor Cocktail EDTA-Free [Roche]) for 20 min at room temperature and centrifuged at 13,000 rpm at 4℃ for 30 min to clear all cell debris ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
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bioRxiv - Developmental Biology 2024Quote: ... The pIEx-4-BmU6-Cad96ca-gRNA-Cas9-GFP-P2A-Puro or pIEx-4-BmU6-Fgfr1-gRNA-Cas9-GFP-P2A-Puro recombinant vectors were transfected into HaEpi cells with transfection reagent (Roche, Basel, Switzerland). After 48 h of vector transfection (cells can be observed to express green fluorescent protein) ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Immunology 2021Quote: ... 20 mM Tris pH 7.5, 1% Triton X-100 supplemented with 1× protease inhibitor and 1× phosphatase inhibitor; Roche, Basel, Switzerland). Lysates were cleared by centrifugation at 10,000 r.p.m ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...
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bioRxiv - Biochemistry 2019Quote: ... 1 % Tween-20 and 1 mM β-mercaptoethanol supplemented with 1 mg/mL lysozyme and protease inhibitor cocktail tablet (Roche, Switzerland) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Tween 20) for 20 min followed by 1 h incubation with alkaline phosphatase-anti-digoxigenin antibody (1:500, Roche, 1093274). Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in 1 ml of blocking solution (1:1 (v/v) of Western Blocking Reagent (Roche, Cat#11921681001) and PBT ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, 1x Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 0.5 mM PMSF, 1 mM DTT and 1×Complete inhibitor tabs, Roche, for 15 min), then treated with 10% NP40 and vortexed ...
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bioRxiv - Developmental Biology 2022Quote: ... 5x SSC, 0.05mg/ml Heparin, 0.25% Tween20, 1% SDS, 1 mg/ml Salmon Sperm DNA, 1 mg/ml Roche blocking buffer powder) containing oligo probes at a concentration of 1μg/120μl and hybridized for two days at 43°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated with the secondary antibodies 1:1000 in PBST supplemented with 1 % DMSO and 1 % blocking reagent (Roche, #1096176) overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... If stained with phalloidin (1/500, Scientific Laboratory Supplies P1951) and DAPI (1/1000 in PBS from 1 mg/ml stocks, Roche 10236276001), or just DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 μl of the lysis buffer was added to each 6-well plate along with 1×Protease Inhibitor Cocktail (PIC, Roche cOmplete). The cells were incubated in a rocker at 4°C for 1hr ...
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bioRxiv - Cell Biology 2019Quote: ... ∼100 µl glass beads and 100 µl of lysis buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 15 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to each dried pellet ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were blocked with 3% skim milk in TBS for 30 minutes and then probed with mouse anti-GFP (1:1,000, Roche) or rabbit anti-aldolase (Mesén-Ramírez et al. ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... and 100 µL protein breakage buffer (50 mM Tris-HCl pH 8.0, 1 mM EDTA, 20 mM Tris pH 9.5, 3 mM DTT, 1X cOmplete EDTA-free inhibitor cocktail [Roche]) were added to the samples ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected according to the manufacturer’s protocol at a µL lipofectamine: µg plasmid ratio of 3:1 (X-tremeGENE 9, Roche). After 48 hours ...
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bioRxiv - Physiology 2023Quote: ... or 50 Tris-HCl pH 7.5, 1 phenylmethylsulfonyl fluoride, 3% SDS (RyR, SERCA, CaMKIIδ-C273S) and supplemented with cOmplete protease inhibitor (Roche). Lysates were then incubated on ice for 15 min and centrifuged for 15 min at 15000 rcf at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... pellets were resuspended in 100 μl of lysis buffer (50 mM Tris, pH 7.5, 1 mM EDTA, 3 mM DTT, and 1X cOmplete protease inhibitor cocktail [11836145001, Roche]) and lysed on a beadbeater for 5 min at room temperature with 100 μl of acid-washed glass beads ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM magnesium chloride) supplemented with 1 cOmplete protease inhibitor cocktail tablet per 50 ml of lysis buffer (Roche) and mechanically disrupted in a Dounce homogenizer ...
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bioRxiv - Plant Biology 2022Quote: ... 1 X protease inhibitor cocktail and 1 X phosphatase inhibitor cocktail from Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... reaction buffer containing 1 μL lambda phosphatase plus 1×phosphatase inhibitors cocktail (Roche) in at 30°C for 30 min with gently shaking ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mM EGTA and 1 mM DTT) supplemented with cOmplete protease inhibitors (Roche), benzonase (Merck ...
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bioRxiv - Immunology 2021Quote: ... and 10% glycerol) containing 1 mM PMSF and 1 × protease inhibitor cocktail (Roche). Then ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mM EGTA and 1 mM DTT) supplemented with complete protease inhibitors (Roche), benzonase (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EGTA and 1% Triton X-100 with protease/phosphatase inhibitor (Roche) mixture ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) with 1 mM PMSF and protease inhibitor cocktail tablets (Roche) by vigorous shaking in a fast prep cell breaker (Bio 101 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Fifty micrograms of total protein were loaded onto SDS-PAGE gels and separated by electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA and supplemented with 1× Complete protease inhibitor cocktail (Roche) by using a Teflon pestle (Schuett Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 mM EDTA and 1×Complete EDTA free-tablet (Roche Diagnostics, Basel, Switzerland) in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
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bioRxiv - Microbiology 2024Quote: ... Lysozyme 1 mg/ml and 1 tablet of EDTA-free protease inhibitor (ROCHE). Cells were broken passing twice through an Emulsiflex C5 (ATA scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... using a 1:1 mixture of oligo dT and random hexamer primers (Roche). Quantification was performed on a LightCycler 480 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was lysed with Lysis buffer (50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton, 1 × Roche inhibitor tablet) on ice for 15 minutes and centrifuged at 8000 rpm for 5 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissues were minced into 1×1 mm fragments and enzymatically dissociated in HBSS containing 1 mg/ml collagenase A (#11088793001; Roche, Basel, Switzerland) and 1 μg/ml DNase I (#07900 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in 1500 μl ice cold RIPA buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM EDTA, Roche cOmplete and 1 mM PMSF). Cell lysates were clarified by centrifugation (13000 x g at 4°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... The cross-linking reaction was quenched by incubating the cells with 0.125 M glycine for 5 min with mild agitation at room temperature followed by centrifugation at 3,000 rpm for 5 min at 4°C with a subsequent PBS wash (containing 0.01X protease inhibitor cocktail or PIC; #11836170001, Roche Applied Science, Indianapolis, USA). Following the complete removal of PBS ...