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Citations for Roche :
9201 - 9250 of 10000+ citations for Cow Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... For enteric glial cell experiments were performed by hypotonic lysis in 200 µl of buffer A (20 mM HEPES, pH 8.0, 1 mM DTT, 0.1% NP-40 and a proteinase inhibitor cocktail from Roche). The cell lysates were incubated on ice for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Fab fragments diluted 1:10,000 in the blocking buffer for 30 minutes at room temperature (Roche, Cat#11093274910) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in homogenization buffer (0.32 M sucrose, 5 mM HEPES pH 7.4, 1 mM EDTA) containing protease inhibitor Complete (Roche) and then homogenized by multiple passing through a 0.24 mm syringe.
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bioRxiv - Microbiology 2022Quote: ... were incubated at 4°C with hypotonic lysis buffer (10 mM Tris–HCl pH 8.0, 1 mM KCl, 1.5 mM MgCl2, with protease inhibitors (ROCHE)) for 2 hours while rotating ...
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bioRxiv - Plant Biology 2022Quote: ... Cell pellets were thawed on ice and resuspended in lysis buffer (20 mM sodium phosphate pH 6.8, 10 mM DTT, 1 mM EDTA, and 0.25× protease inhibitor cocktail [Roche]). Cells were sonicated on ice and centrifuged for 10 min at 500 g and 4 °C to remove intact cells and cell debris ...
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bioRxiv - Epidemiology 2020Quote: ... Real-time PCR assays were performed with LightCycler® 480 Probe Master Mix 1× (Roche Applied Science, Germany), using 200 nM of primers and probes in a final volume of 12 µl and 2 µl of control DNA were added ...
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bioRxiv - Microbiology 2019Quote: ... and HIV-1 p24 levels in supernatants were measured on the HIV COBAS 8000 platform (Roche Diagnostics, Switzerland). Viral growth kinetics (VGK ...
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bioRxiv - Developmental Biology 2019Quote: ... fetal or newborn stage thymi were dissected and digested in 1 mg/mL collagenase/dispase (Roche, Basel, Switzerland), and passed through a 100-μm mesh to remove debris ...
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bioRxiv - Pathology 2019Quote: ... Lungs were excised and homogenized in 1 mL PBS containing cOmplete Proteinase Inhibitor Cocktail (Roche Diagnostics, Mannheim, Germany) then centrifuged at 4°C to remove cell debris ...
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.3 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc., A Roche Company, Canada) and 0.75 μL of both forward and reverse 10 μM oligos ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.3 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc., A Roche Company, Canada) and 0.75 μL of both forward and reverse 10 μM oligos ...
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bioRxiv - Molecular Biology 2019Quote: Crosslinked nuclei were extracted in 0.8ml modified RIPA lysis buffer (50mM Tris, 150mM NaCl, 0.5% sodium deoxycholate, 0.2% SDS, 1% NP-40, supplemented with: 1x antiprotease (Roche), RNasin (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was diluted 1:10 before usage and amplified on a LightCycler 480 (Roche Diagnostics, Basel, Switzerland) using the Applied Biosystems SYBR Green Master Mix (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Whole cell extracts were prepared in HEPES whole cell lysis buffer (20 mM HEPES, pH 7.4, 1% TX-100,1x phosphoSTOP complete inhibition cocktail tablets [Roche] ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and incubated with G6B antibody diluted 1:100 in Ventana’s DISCOVERY antibody diluent (Roche Cat#760-108) for 60 minutes at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... After a series of washes the probes were detected using FITC conjugated anti-Digoxigenin antibody (1:20, Roche) amplified with anti-sheep Alexa Fluor 488 (1:100 ...
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bioRxiv - Genetics 2019Quote: ... 150 µl of zirconia beads and 150 µl of lysis buffer (50 mM Tris [pH 7.5], 1 mM EDTA, 2.75 mM DTT, 1x PhosSTOP (Roche), 0.48 µg/ml Pefabloc SC (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in wash buffer [(10 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, protease inhibitor (Roche)] and rotated for 10 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... for Quantitative RT-PCR (Supplemental Table 1) and amplified in a Roche Light Cycler 480 (Roche, Basel, Switzerland). Relative expression was quantified using Ct values ...
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bioRxiv - Microbiology 2019Quote: ... The total protein pellets were resuspended in 1 mL NP-40 with protease inhibitor cocktail (Roche Diagnostics GmbH). Samples were pre-cleared using Protein A/G plus agarose beads (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h with rat anti-HA high affinity monoclonal antibodies (Roche Applied Science, Penzberg, Germany) at 1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA and 0.5% NP-40 supplemented with Complete Mini EDTA-free Protease inhibitor cocktail tablets (Roche)) and left to incubate for 20 minutes on ice ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche). 150 ul of sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 3 µg of antibody conjugated to 10 µl of Dynabeads ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 1ml of cold Lysis Buffer 1 (10mM Tris pH 8.0, 10mM NaCl, 0.2% IGE-PAL, Protease inhibitor (Roche)) and incubated on ice for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Biochemistry 2020Quote: ... PFN1-KO or PFN2-KO) were split 1:3 and the next day transfected using XtremeGene 9 (Roche) with 5 μg V5-NAA80 (M23L ...
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bioRxiv - Molecular Biology 2019Quote: ... High Salt buffer (50 mM HEPES pH 7.5, 500 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF)) supplemented with EDTA-free protease inhibitor cocktail and phosphatase inhibitors cocktail (Roche). Tissue lysates were centrifuged at 14,000 rpm for 20 min at 4°C twice before using the supernatant for western blot studies ...
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bioRxiv - Developmental Biology 2019Quote: ... All three vectors were transfected into DLD-1 TIR1 cells using Xtreme Gene 9 DNA transfection reagent (Roche). Cells were sorted based on their YFP fluorescence and single clones were isolated ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, Roche mini-tablet protease inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4 °C with alkaline phosphatase–conjugated anti-digoxigenin antibodies in TBTX (1:2000; Roche). Following extensive washing in TBTX ...
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bioRxiv - Cell Biology 2019Quote: Control BMSCs were lysed by incubation with RIPA buffer (Pierce) and 1 mM protease inhibitors (Complete Mini, Roche). Samples were incubated with 5X Lane Marker Reducing sample buffer (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... Plasma viral load in ART-treated PLWH was measured using the Amplicor HIV-1 monitor ultrasensitive method (Roche).
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1 × protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 μl/10 mg) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extraction buffer (60 mM Tris-HCl [pH 8.8], 2% [v/v] glycerol, 0.13 mM EDTA [pH 8.0], and 1× protease inhibitor cocktail complete from Roche) was added to the homogenized tissues (100 µl/10 mg) ...
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bioRxiv - Microbiology 2021Quote: ... and polymerized by UV light prior to labeling with anti-HA antibody (Roche, clone 3F10, 1:40 dilution) and 10nm gold bead conjugated goat anti-rat (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells are pelleted and resuspended in 1 mL of Lysis Buffer (200 mM NaCl, 25 mM HEPES, 1 mM MgCl2, 0.2 mM EDTA, 0.5% NP-40, 1X Roche complete inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2021Quote: Lysates for Western-blot were made in a 1% triton buffer including cOmplete protease inhibitor cocktail (Roche 11697498001) and Western-Blot and flow cytometric staining was performed as described previously (41),(42) ...
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bioRxiv - Microbiology 2020Quote: ... 0.05 μL of 20 μg/μL (1 μg BSA/reaction) of nonacetylated bovine serum albumin (BSA, 10711454001, Roche), 1 μL SuperScript™ III RT/Platinum Taq Mix and 1.85 μL of primer/probe mix (2019-nCoV CDC EUA Kit ...
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bioRxiv - Microbiology 2021Quote: ... A 1 uL aliquot was used to amplify the V1-V3 region using HiFi HotStart ReadyMix (KAPA BioSystems) and the Illumina PCR protocol ...
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bioRxiv - Genomics 2019Quote: ... Probes were bound to 2 µg nuclear extracts pre-incubated with 1 µg poly d(I-C) (Roche) or 100-750ng YY1 full-length recombinant protein (31332 ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of hybridized probes was performed with anti-DIG antibodies AP fragments (Roche, Basel Switzerland; dilution 1:1000) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and lysed by resuspension in lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 0.5% NP-40, and protease inhibitor [Roche]) with rotation at 4°C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed twice with PBS before lysis in 0.1ml lysis buffer (1% Triton X-100, 0.1% SDS, PBS, Complete EDTA-free protease inhibitor [Roche]). Total protein concentration of lysates was quantitated using the Pierce BCA assay (cat no 23225 ...
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bioRxiv - Immunology 2019Quote: ... and cells were lysed in plate in RIPA buffer (50 mM Tris, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1x Roche protease inhibitor tablet [no EDTA] ...