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Citations for Roche :
851 - 900 of 10000+ citations for Mouse EGF Latrophilin Seven Transmembrane Domain Containing Protein 1 ELTD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... the tissue was finely chopped on ice for approximately 1 minute using 600 μL of pre-chilled Nuclei Isolation Buffer containing 0.4U/μL RNase inhibitor (Roche, Protector RNase Inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2024Quote: Cells were harvested and protein was extracted using lysis buffer (20mM HEPES pH7.4, 2mM MgCl2, 40mM NaCl and 1% Triton-X) containing complete mini EDTA protease inhibitor (Roche) at 1 in 50 concentration ...
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bioRxiv - Biochemistry 2024Quote: Homogenized lungs were 10-fold diluted starting 1:10 in infection media (DMEM containing 0.1% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Plant Biology 2022Quote: ... Protein detection was carried out using peroxidase-coupled anti-HA-Peroxidase antibodies (Roche, dilution 1/4000), peroxidase-coupled anti-GFP antibodies (Milteneyi ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... Cell lysates (1 mg) were pre-cleared with Protein-G–agarose beads (Roche, catalog number 11719416001) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins with HA-tag(s) were detected using rat mAb-anti-HA 3F10 (Roche, 1:1000) as primary antibody and IRDye® 800CW Goat anti-Rat IgG (Licor ...
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bioRxiv - Neuroscience 2019Quote: ... The aqueous liquid phase containing the nucleic acids was removed and added to the columns of the High Pure RNA tissue kit (Roche Diagnostics, UK). RNA was purified using repeated wash steps and DNAse treatment ...
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bioRxiv - Biochemistry 2023Quote: ... LDH kit and WST-1 reagent were purchased from Roche. Bleomycin sulphate was purchased from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... Protein G-agarose and protein A-agarose beads were from Roche. GFP-Trap®-A beads were from Chromotek (Planneg-Martinsried ...
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bioRxiv - Developmental Biology 2020Quote: ... and sequential incubation with an anti-GFP primary antibody (mouse anti-GFP, 1:200, 11814460001, Roche) and a corresponding secondary antibody (goat anti-mouse Alexa Fluor 488) ...
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bioRxiv - Plant Biology 2019Quote: ... Jas9-VENUS and ACTIN were detected using mouse monoclonal antibodies anti-GFP 1:1,000 (11814460001, Roche) or anti-actin 1:2,000 (A0480 ...
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bioRxiv - Biophysics 2021Quote: ... The following primary antibodies were used at the indicated dilutions: mouse anti-Myc (Roche, 1:3000), goat anti-p75NTR (Neuromics ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used in this study: mouse monoclonal anti-GFP (1:2’000; Roche), anti-HA (clone 16B12 ...
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bioRxiv - Molecular Biology 2019Quote: ... DIG-labeled probes were detected using mouse monoclonal anti-DIG primary antibody (Roche; 1:100 dilution) and a Cy3-labeled goat anti-mouse IgG secondary antibody (Roche ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Western blot analysis was followed with an antibody against GFP (1:1000, mouse anti-GFP, Roche) and goat anti-Mouse IgG-HRP (1:3000 ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... The primary and secondary antibodies used in this analysis were: mouse anti-GFP (1:1000, Roche), rabbit anti-PLK4 (1:500) ...
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bioRxiv - Bioengineering 2023Quote: ... We also switched from mouse anti-HA to rat anti-HA (Roche catalog #3F10, 1:1000) to circumvent antibody cross reactivity issues observed with Olig2 and other cell type markers ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies used for immunoblotting were anti-GFP mouse monoclonal antibody (1:3,000 dilution, Roche, 11814460001) and anti-mCherry mouse monoclonal antibody (1:3,000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... GFP (mouse, Roche, 11814460001 ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA prepared using the KAPA Express reagent (a component of the KAPA Mouse Genotyping Kit from Roche, 07961804001) and analyzed for both CRISPR lesions and repeat size as described below.
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bioRxiv - Plant Biology 2019Quote: ... 4 °C) the cleared supernatants were diluted 1 time using water containing 1x cOmplete ™ EDTA-free protease inhibitor (Roche). Protein extract was incubated overnight with anti-HA antibodies (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... monocytes were harvested using ice-cold lysis buffer (1% Triton X-100, 2% SDS, 150 mM NaCl, 10 mM HEPES, 2 mM EDTA containing protease inhibitor cocktail - Roche). Cell lysates were heated at 100 °C for 5 min in the presence of Laemmli buffer (20% β-mercaptoethanol ...
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bioRxiv - Genetics 2021Quote: ... livers were perfused at low flow for approximately 10 min with Digestion Buffer (low glucose DMEM supplemented with 1 mM HEPES) containing freshly added Liberase TM (32 μg/mL; Roche). Digestion was stopped using Isolation Buffer (low glucose DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: Cell pellets of 1 × 106 cells were resuspended in 100 μl RIPA lysis buffer containing a protease inhibitor cocktail (Roche). Samples were centrifuged for 5 min to remove cell debris and stored at −20°C until use for Western blot analysis.
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bioRxiv - Genomics 2020Quote: ... cell pellets of approximately 1-5 million cells digested overnight at 50C in 500 μl lysis buffer containing 100 μg/ml proteinase K (Roche), 10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100, pH 7.4, containing phosphatase inhibitors (10 mM NaF, 1 mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Immunology 2022Quote: ... In short lungs were minced with scissors and incubated in a digestion cocktail containing 1 mg/mL collagenase A (Roche) and 30 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using RIPA buffer (50 mMTris–HCl, 10 mM MgCl2, 20% Glycerol and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche). Protein concentration was determined by BCA assay (Pierce ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended in ice-cold NP-40 Buffer (150mM NaCl, 1% NP-40, 50mM Tris pH8) containing EDTA free protease inhibitors (complete cocktail, Roche) and lysed using a 1ml tissue homogenizer (Fisher) ...
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bioRxiv - Immunology 2022Quote: One million cells were collected by centrifugation and resuspended in cold lysis buffer (1% Triton X-100 in PBS) containing protease inhibitors (Complete protease inhibitor cocktail, Roche) and was incubated on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed with PBS and crosslinked for 30 min in 2 ml ice cold 1% formaldehyde containing 2X Complete EDTA-free Protease Inhibitor Cocktail (Roche). Cross-linking was stopped by the addition of glycine to 333 mM ...
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bioRxiv - Cell Biology 2022Quote: ... collected and lysed with NP-40 extract buffer (50 mM Tris, pH 8, 150 mM NaCl, 1 % NP-40) containing protease inhibitors (#11873580001 Roche) and 30 µg of lysate was migrated in 10 % SDS-PAGE gels (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 30 min at 37 °C in a solution (1 ml per well) containing 10 U/ml of dispase II (Roche) and 125 U/ml of collagenase type IV (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
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bioRxiv - Neuroscience 2022Quote: ... Hippocampi were isolated and stored in 10 volumes of sucrose buffer 1 (320mM sucrose, 1mM NaHCO3, 1mM MgCl2, 0.5mM CaCl2, 1µM PMSF) containing protease inhibitor cocktail (Roche, Mannheim, Germany). Tissues were homogenized for 10 strokes using a motor-driven 2 ml Potter-Elvehjem homogenizer fitted with a Teflon pestle ...
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bioRxiv - Neuroscience 2019Quote: ... Remaining pellets were washed with PBS and resuspended in 1% sarkosyl in PBS containing complete mini protease inhibitor cocktail (Roche), vortexed for 30 sec and incubated at room temperature for 1 hr ...
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bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
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bioRxiv - Molecular Biology 2020Quote: MtDNA copy number was analyzed in duplicate by quantitative real-time PCR using 2 μl of 1/400-diluted SacI-treated total DNA in a 10 μl reaction containing 0.2 μM forward and reverse primers and 1× KAPA SYBR FAST qPCR Master Mix for LightCycler 480 (KAPA Biosystems) in a LightCycler 96 instrument (Roche) ...
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bioRxiv - Genetics 2021Quote: ... cells were collected in lysis buffer (TBS + 1% Triton X-100) containing protease inhibitors (Complete Mini EDTA-Free, Roche 04693159001), briefly sonicated ...
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bioRxiv - Immunology 2020Quote: Cell lysates from flow-sorted bone marrow osteoclast progenitors grown in presence of M-CSF and RANKL over time were harvested in 1% NP40 lysis buffer containing 1X cOmplete Protease Inhibitor cocktail (Roche). Samples were separated by SDS-PAGE and transferred to nitrocellulose membrane ...
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bioRxiv - Microbiology 2021Quote: ... the parasites were re-suspended in 1xPBS containing 1 mg/ml soybean trypsin inhibitor and 25x protease inhibitor cocktail (Roche) before the parasites were pelleted and lysed in lysis buffer (4% SDS ...