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Citations for Roche :
851 - 900 of 5875 citations for IL 1 beta Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Genetics 2019Quote: ... The supernatant was removed and the pelleted cells lysed by 3-cycles of freezing/thawing in 150 μl lysis buffer (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 1 mM EDTA, 0.1% IGEPAL, 1 mM DTT, 1x Complete Protease Inhibitor [Roche]). Cell extracts were centrifuged for 5 min at 12000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Microbiology 2020Quote: ... 100 mM Tris-HCl pH 8.0, 5% glycerol, 1 mM DTT, 0.1% CHAPS, 1 μg/mL avidin, cOmplete, EDTA-free protease inhibitors [Roche]), rotated at 4°C for 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... Soluble proteins were extracted using 50 μl of protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT and 1×Complete protease inhibitors [Roche]), incubated on ice for 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) for 1-2 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100, 0.1% deoxycholate Na, 0.1% SDS, 1 mM AEBSF [Euromedex] and cOmplete EDTA-free [Roche]) with glass beads (diameter ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). Clarified lysates were incubated with 10 µl of HPC4 coupled beads (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 5mM CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) and 1 tablet of Protease Inhibitor Cocktail (ROCHE, cOmplete™). The preparation was sonicated for 120 s on ice ...
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bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2019Quote: ... Heat induced epitope retrieval was applied for 1 hour using cell conditioning 1 buffer (CC1, Roche, #950-500) followed by the application of anti-CD31 (Histobiotec ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Plant Biology 2022Quote: ... o,1% SDS and 5mM DTT) supplemented with a 1:100 dilution of plant protease inhibitor cocktail (Roche) was added to the ground samples and centrifuged 20 minutes at 6,000 x g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche; 3115828001) in lysis buffer (50 mM Tris HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with maltose (20 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... 2.7 mM KCl, 0.1% NP40, 1 mM EDTA, 1mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) and eluted with glutathione (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with glass beads in 250 µl RIPA buffer (25mM Tris/HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, Roche complete protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 0.1% NP-40, 1 mM DTT, 1 mM PMSF, 1.3 mM benzamidine, protease inhibitor tablets [Roche]) using 300 μg/mL lysozyme for 20 minutes followed by sonication ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 mM CTP, 1 mM GTP, 0.65 mM UTP, 0.35 mM Biotin-16-UTP, pH 7.5; Roche/Sigma) and ∼40 U of either T7 (Roche/Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclear pellet was then resuspended in RIPA buffer (1X PBS, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, supplemented with 1× protease inhibitor mixture Complete (Roche)) and chromatin was sheared to a range of 200-500bp by using a Bioruptor instrument (Diagenode ...
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bioRxiv - Biochemistry 2023Quote: ... and soluble FMS like Tyrosine kinase 1 (sFlt-1) on a Cobas e411 analyser (Roche Diagnostics, Mannheim, Germany).27 The serum hCG concentrations of the samples from the six individuals used in this study were compared against the respective LC-MS/MS derived values.
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 mM MgCl2, 1 mM EDTA, 1 mM DTT, 0.075% NP-40, 1x protease/phosphatase inhibitor cocktails, Roche) and incubated for 10 minutes at 4°C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then washed with ice-cold PBS two times and lysed in 300 μl RIPA buffer (50 mM TRIS pH 8, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, Roche Complete protease inhibitors ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.15M NaCl) for 1 hour at R/T and then incubated with anti-DIG antibody (Roche; 1:5000) overnight ...
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bioRxiv - Plant Biology 2020Quote: ... and 1× protease inhibitor cocktail (Roche, USA)] ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 protease inhibitor tablet (Roche, Cat. 11873580001)) using a dounce homogenizer ...
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bioRxiv - Cancer Biology 2021Quote: ... WST-1 assays (Roche Diagnostics, Mannheim, Germany) were performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM protease inhibitors cocktail (Roche, 04693159001)) on ice for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Fluorescein-POD (1:2000; Roche #11426346910), or anti- Dinitrophenyl-HRP (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (Roche, # 11867431001, 1/1000), mouse anti-GFP (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (Roche, # 11814460001, 1/1000), rabbit anti-calreticulin (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (1:1000, Roche 11814460001), goat anti-SYP-1 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (1:200, 3F10, Roche) followed by anti-Rabbit/Mouse/Rat Alexa-dye coupled antibodies (1:400 ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-HA (1:50; Roche 3F10), rabbit anti-β galactosidase (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-HA (1:300; Roche 3F10). Luminal chitin was detected using the chitin-binding domain from Bacillus circulans chitinase A1 conjugated with SNAP-Surface AlexaFluor 488 or 563 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 cOmplete Protease Inhibitor Cocktail tablet (Roche), 1 mg/mL lysozyme ...
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bioRxiv - Immunology 2019Quote: ... collagenase (1 mg/ml collagenase II; Roche) and DNase I (100 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... or anti-digoxigenin-AP (1:4000, Roche/Sigma-Aldrich 11093274910 ...
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bioRxiv - Microbiology 2021Quote: ... and/or 1:500 anti-HA (Roche), then 1:1000 Alexa Fluor 488-conjugated anti-rat IgG (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µl proteinase K (Roche, Cat 10910000) was then added and the mix was incubated at 55℃ for 90 min to elute DNA from beads.
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bioRxiv - Molecular Biology 2022Quote: ... with 1× cOmplete protease inhibitor cocktail (Roche). Supernatant after centrifugation was incubated with Ni-NTA Agarose (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... containing collagenase A (1 mg/ml, Roche), DNase I (20 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... and Dispase II (1 mg/ml, Roche) overnight at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... anti-HA rat (Roche, 11867423001; 1:100), anti-Brp mouse (nc82 ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-HA (1:500, 11867423001, Roche) or monoclonal mouse anti-GFP antibody (1:1000 ...