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Citations for Roche :
851 - 900 of 8781 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Tween-20 0.3%) for 1 hour and finally incubated overnight at 4°C with anti-Dig-POD antibody (Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tissues were washed in three changes of 0.2× SSC at 44°C for 15 minutes each and blocked in 4×SSC containing 1% blocking reagent (Roche) in 4× SSC for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... were homogenised with 1:4 weigth per volume diethylamine (DEA) buffer (50 mM NaCl, 0.2% diethylamine, pH 10, cOmplete Protease InhibitorsTM, Roche). After 60 min at 130000xg and 4°C ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then rinsed with PBS and lysed at 4°C in Buffer A (1% Triton X-100, 0.5% NaDOC and 1X cOmplete Protease Inhibitor Cocktail (Roche) in PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Washed three times for 30 minutes each in TNTT at 4 ℃ to remove methanol.Blocked with 1% blocking reagents (Roche) in TNTT buffer for 2 hours at 4 ℃ to prevent no specific binding ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 5 mM imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 5 mM imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 5 mM imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol and 5 mM Imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and 5 mM imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and 5 mM Imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Biochemistry 2021Quote: ... 5% glycerol and 5 mM imidazole supplemented with EDTA-free protease inhibitor (Roche) and lysed by ultrasonication ...
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bioRxiv - Developmental Biology 2021Quote: ... Planarians were blocked in Blocking Solution (5% heat inactivated horse serum, 5% Roche Western Blocking Buffer in TNTx [0.1 M Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... transferrin (5 μg/mL) and sodium selenite (5 ng/mL) (Roche Diagnostics, Germany), 100 units/mL of penicillin ...
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bioRxiv - Developmental Biology 2023Quote: ... Planarians were blocked in Blocking Solution (5% heat inactivated horse serum, 5% Roche Western Blocking Buffer in TNTx [0.1 M Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight at 4°C in primary antibody dilutions in freshly prepared BBT+ buffer (PBST + 1% BSA + 0.5 mM Spermidine + 2 mM EDTA + 1 large Roche complete EDTA-free tablets). Primary antibody was replaced with BBT+ buffer and quickly washed twice ...
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bioRxiv - Cancer Biology 2020Quote: ... grade 2 (Roche), homogenizing for 20min with a plastic Pasteur pipette ...
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bioRxiv - Genomics 2020Quote: ... washed with 500 μL buffer MB#1 (10 mM Tris-HCl, pH 7.5, 50 nM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche complete EDTA-free (Roche diagnostics ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed embryos were placed into hybridization buffer (50% formamide, 5×SSC, 1 mg/ml yeast tRNA (Roche, 10109223001), 100 μg/ml heparin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked with 5% non-fat milk TBST and probed with primary anti-GFP (Roche, 1:1000) and secondary HRP- conjugated anti-mouse antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: Adherent cells were washed twice with PBS and then scraped and digested on ice for 30 min with a homemade RIPA (50 mM Tris-HCl pH 7.5, 15 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS, supplemented with Protease Inhibitors [Roche] and Phosphatase Inhibitors [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 500 mM NaCl, 0.4% SDS, 5 mM EDTA, 1 mM DTT, and 1x complete protease inhibitor [Roche]) and sonicated ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3% (v/v) Tween-20) for 5 min under agitation and next blocked in 1× blocking buffer (Roche) for 30 min at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were blocked with 5% milk dissolved in TBST and probed with primary anti-HA (Roche, 1:2000), anti-Flag (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... 1.5 mM magnesium acetate] containing 5 mM dithiothreitol (DTT) and 1 × EDTA-free Complete Protease Inhibitor tablets (Roche) by tapping and inverting the tubes ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in homogenization buffer (0.32 M sucrose, 5 mM HEPES pH 7.4, 1 mM EDTA) containing protease inhibitor Complete (Roche) and then homogenized by multiple passing through a 0.24 mm syringe.
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Genomics 2022Quote: ... incubated with 1ml MB#1 buffer (10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mM CaCl2, 0.2% NP-40, 1x Roche cOmplete EDTA-free (Roche diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 mM NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM DTT, 5% glycerol, 1x protease/phosphatase inhibitor cocktails, Roche). Nuclear lysates were diluted with 2 volumes of dilution buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM MgCl2, 0.25% IGEPAL CA-630, 1 mM DTT, cOmplete protease inhibitor [Roche]) and mixed with 200 µL of 3 µg µL−1 nuclear extract and 500 µL PBB+ buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 8.0, 0.25% IGEPAL CA-630, 1 mM DTT, 5 mM MgCl2, cOmplete protease inhibitor by Roche). Per replicate and construct ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM Tris-HCl pH 7.5, 0.5% IGEPAL CA-630, 5 mM MgCl2, 1 mM DTT, 1x protease inhibitor EDTA free [Roche]) and mixed with 50 µL PBB-buffer-equilibrated MyOne Streptavidin C1 Dynabeads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Bacterial pellets were collected via centrifugation at 4°C and 5,000g and resuspended in ice-cold lysis buffer (50 mM MES pH 6.8, 1 mM EGTA, 0.2 mM MgCl2, 5 mM DTT, Roche complete protease inhibitor ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortor and pestle and resuspended in 30 mL extraction buffer 1 (10 mM Tris-HCl, pH 8.0, 5 mM βmercaptoethanol, 0.4 M sucrose, protease inhibitor cocktail (cOmplete, Roche), vortexed ...
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bioRxiv - Molecular Biology 2024Quote: ... 1mM MgCl2, 0.1% NP-40, 5 mM DTT, 0.5 mM PMSF, 1× EDTA-free protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 0.5 % SDS, 1#x00D7; protease inhibitor cocktail from Roche). The resulting nuclei were spun down ...
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bioRxiv - Cell Biology 2022Quote: ... The HEK 293T cells were mixed with 1 μg of mouse Ano9 cDNA (mAno9-pEGFP-N1) and 3 μl FuGene HD (Roche Diagnostics). The transfected cells were plated onto glass coverslips that were kept in a 35-mm round Petri dish ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested either for 1 hour at 37°C in a digestion mix of 3 mg/ml collagenase type A (Roche, 11088793001) and 25 μg/ml DNAse (Invitrogen ...
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bioRxiv - Genetics 2019Quote: ... Coverslips were blocked in 3% BSA (wt/vol) and digoxigenin was detected with sheep anti-digoxigenin FITC 1/50 (Roche, 11207741910) followed by rabbit anti–sheep FITC 1/100 (Vector Laboratories ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each eluate (3 µl) was subjected to PCR amplification in 50-μl reactions containing 1 KAPA HiFi HotStart Uracil+ReadyMix (Kapa Biosystems) and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Molecular Biology 2024Quote: Transfected COS-7 cells were scraped in ice cold PBS containing Complete protein inhibitor cocktail tablets (Roche), centrifuged at 200 x g for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The region around the E-Box was amplified with primers outside of the homology regions using Kappa HiFi HotStart ReadyMix (Roche) and cloned into pCR-Blunt II-TOPO (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Real time quantitative PCR was performed on the E gene as described recently (Corman et al., 2020) on a Lightcycler LC480 (Roche).