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Citations for Roche :
851 - 900 of 5876 citations for 6 Hydroxy 1 iodonaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 1.5 mM MgCl2) with 1 mM PMSF and 1× EDTA-free protease inhibitor cocktail (PIC, Roche, 11873580001) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... in blocking buffer for 1 hour at room temperature followed by 1 hour of DAPI (Roche, # 33495822) staining ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was then resuspended in HEGX Buffer (20 mM HEPES-KOH, pH 7.2, 0.8M NaCl, 1 mM EDTA, 10% glycerol, 0.2% Triton X-100 and 1 Roche Complete Protease Inhibitor Tablet ...
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bioRxiv - Genomics 2022Quote: ... 1 μL of 1:10 diluted cDNA was used in a 10 μL SYBR Green (Roche, 04887352001) qPCR reaction on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with 1:500 mouse anti-actin26 and 1:1000 rat anti-HA (3F10, Roche) followed by 1:1000 anti-mouse Alexa 488 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 1 % Nonidet-P 40) supplied with 1 tablet/50 mL c0mplete EDTA-free Protease Inhibitor Cocktail (Roche), per mg cell pellet and rotating on a wheel at 4°C for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Purified parasites treated at 12°C for 1 h with freshly prepared 1 mg/mL pronase (Roche)/0.01% saponin/PBS before washing ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100 and 1 tablet of protease inhibitor for every 10ml of lysis buffer (Roche cOmplete ULTRA Tablets ...
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bioRxiv - Neuroscience 2019Quote: ... 1% Triton X-100 and 0.1% SDS) with protease inhibitor (Roche/1 tablet in 10 mL RIPA). Sample homogenates were centrifuged for 5 min at 4°C at 13,000 G ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% NaDOC, 1% Triton X-100, 0.1% SDS, 0.25% sarkosyl, 1 mM DTT, protease inhibitors from Roche). The cells were sonicated on ice to shear DNA to 300-500 bp ...
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bioRxiv - Immunology 2020Quote: Tissue was harvested and incubated in a digestion cocktail containing 1 mg ml−1 collagenase A (Roche) and 30 μg ml−1 DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM EGTA and 1% Triton X-100 supplemented with complete EDTA-free protease inhibitor (Roche, 11873580001) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 1 hour at RT with the secondary antibodies together with DAPI (1:5,000; Roche), all diluted in Dako Antibody Diluent ...
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bioRxiv - Microbiology 2022Quote: ... at a 1:1 ratio in a MagNA Pure 96-well deep-well extraction plate (06241603001, Roche), covered with a MagNA Pure Sealing Foil (06241638001 ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed sections were rehydrated and incubated with primary antibodies against ER (SP1; 1:1; Roche, Basel, Swiss), GATA3 (L50-823 ...
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bioRxiv - Neuroscience 2023Quote: ... The agarose resin was washed once with high salt buffer (50 mM Tris HCl pH 7.4, 1M NaCl, 1 mM EDTA, 1% Nonidet NP-40, 0.1% SDS, 0.5% Sodium deoxycholate, Roche cOmplete EDTA-free Protease Inhibitor Cocktail and RNase Inhibitor ...
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bioRxiv - Physiology 2023Quote: ... pregnant mice were anesthetized (1:1:0.16 Fentannyl [Fentadon, Dechra Vetinary Products UK], Midazolam [Hypnovel®, Roche, UK] and Ketamine [Ketavet ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were extracted into a Triton X-100 buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM EDTA, 1% Triton X-100, supplemented with 1× protease inhibitors - Roche). Extracts were incubated on ice for 30 min then clarified by centrifugation at 12,000g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed 2 x with PBS2+ and 4 ml lysis buffer (50 mM Tris-HCl, pH 7.4, 300 mM NaCl, 1% Triton X-100, 0.1% BSA, 1 x protease inhibitors [Roche]) was added to the washed samples ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was replaced with RIPA buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM MgCl2, 1% NP-40, 0.5% sodium deoxycholate, 1x complete protease inhibitor [Roche]) including 1% SDS ...
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bioRxiv - Developmental Biology 2021Quote: Cell pellets were incubated 20 minutes with RIPA lysis buffer (50 mM Tris-HCl pH7.5, 300 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 1X Roche COMPLETE Mini EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... for Western blot analysis probing for HA and FLAG epitopes using 1° antibody = 1:500 mouse αHA (Roche), or 1:500 mouse αFLAG (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... previously washed three times with RIPA 0.1% SDS (1X PBS, 1% IGEPAL-C630, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA and 1X protease inhibitor cocktail; Roche). Anti-FLAG M2 Magnetic Beads (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with RIPA buffer (25 mM Tris HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche]). The lysates were subjected to SDS-PAGE under denaturing conditions in 12% polyacrylamide gels using Bio-Rad Mini PROTEAN electrophoresis system (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Ground cell pellet was collected and resuspended end-over-end for 1 h in lysis buffer (160 mM HEPES pH 7.5, 400 mM NaCl, 0.25 mM PMSF, 1 Roche cOmplete™ Protease Inhibitor Cocktail tablet ...
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bioRxiv - Immunology 2022Quote: ... and then minced and dissociated in digestion buffer (RPMI containing collagenase (1 mg ml−1 collagenase D; Roche), DNase I (100 μg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... containing Phosphatase (1 tablet per 3.3ml lysis buffer) and Protease (1 tablet per 16.67ml lysis buffer) inhibitor (Roche cOmplete tablets ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 viral load was determined using the COBAS® AmpliPrep/COBAS® TaqMan HIV-1 test (Roche). PBMCs were purified using BD Vacutainer® CPT™ Mononuclear cell preparation tubes (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 1 mM PMSF, Roche protease and phosphate inhibitor cocktails ...
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bioRxiv - Immunology 2020Quote: ... DNAse I (0.2 mg ml−1 dissolved in PBS) and Liberase (0.65 Wuensch units ml−1, both Roche, dissolved in HBSS supplemented with 8 % FCS) ...
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bioRxiv - Physiology 2020Quote: ... 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, protease inhibitors [Roche], phosphatase inhibitors [Roche]). Lysates were incubated for 15 minutes on ice and centrifuged twice at 15,000 x g for 20 minutes at 4°C to remove insoluble material ...
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bioRxiv - Genetics 2019Quote: ... The supernatant was removed and the pelleted cells lysed by 3-cycles of freezing/thawing in 150 μl lysis buffer (20 mM Tris-HCl pH 7.6, 150 mM NaCl, 1 mM EDTA, 0.1% IGEPAL, 1 mM DTT, 1x Complete Protease Inhibitor [Roche]). Cell extracts were centrifuged for 5 min at 12000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet was suspended in 1 mL of cold lysis buffer (50 mM TRIS-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail ...
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bioRxiv - Microbiology 2020Quote: ... 100 mM Tris-HCl pH 8.0, 5% glycerol, 1 mM DTT, 0.1% CHAPS, 1 μg/mL avidin, cOmplete, EDTA-free protease inhibitors [Roche]), rotated at 4°C for 30 min ...
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bioRxiv - Plant Biology 2019Quote: ... Soluble proteins were extracted using 50 μl of protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Tween-20, 1 mM DTT and 1×Complete protease inhibitors [Roche]), incubated on ice for 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-c-Myc primary antibody solution (GenScript, 1: 2,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) overnight at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche]) for 1-2 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100, 0.1% deoxycholate Na, 0.1% SDS, 1 mM AEBSF [Euromedex] and cOmplete EDTA-free [Roche]) with glass beads (diameter ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). 20 µg of GST fusion protein and 20 µl of Glutathione Sepharose 4B Beads (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 150 mM NaCl, 1mM CaCl2, 1% NP40, 0.5% Na Deoxycholate, 0.1% SDS 1% supplemented with protease inhibitor cocktail, Roche). Clarified lysates were incubated with 10 µl of HPC4 coupled beads (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 5mM CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) and 1 tablet of Protease Inhibitor Cocktail (ROCHE, cOmplete™). The preparation was sonicated for 120 s on ice ...
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bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... then resuspended in 4°C homogenization buffer (0.6 M sorbitol, 10 mM Tris pH 7.4, 1 mM EDTA, 1 mM PMSF, 2× Roche cOmplete protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2019Quote: ... Heat induced epitope retrieval was applied for 1 hour using cell conditioning 1 buffer (CC1, Roche, #950-500) followed by the application of anti-CD31 (Histobiotec ...
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bioRxiv - Cell Biology 2022Quote: ... three hearts per experimental group were homogenized into ice-cold isolation buffer (300 mM KCl, 30 mM PIPES pH 6.6, 0.5% NP-40, 1× protease inhibitor [Roche], 1× phos-stop [Roche]), followed by centrifugation at 15,000 rpm for 10 minutes in a tabletop centrifuge at 4°C to remove insoluble proteins ...
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bioRxiv - Plant Biology 2022Quote: ... o,1% SDS and 5mM DTT) supplemented with a 1:100 dilution of plant protease inhibitor cocktail (Roche) was added to the ground samples and centrifuged 20 minutes at 6,000 x g at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche; 3115828001) in lysis buffer (50 mM Tris HCl pH 8 ...