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Citations for Roche :
851 - 900 of 6830 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... diluted in 100 mM ammonium acetate (pH = 4) and digested with Glu-C protease (Roche) at 10:1 protein:enzyme for 1 h at room temperature prior to mass spectrometric analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µM MgSO4 and the protease inhibitor cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Cells were then sonicated and the mixture was clarified by centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were subjected to end-on rotation at 4 °C with anti-GFP (Roche, 11814460001) or anti-FLAG (Merck ...
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bioRxiv - Biophysics 2024Quote: ... PCR reactions were performed as follows: 4 µL of KAPA HiFi HiFi HotStart ReadyMix (Roche) was assembled with 1.6 µL of 1:10 bacteria dilution ...
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bioRxiv - Cell Biology 2024Quote: ... All steps were performed at 4°C and in the presence of Protease inhibitors (Roche) and 1mM TSA in every buffer to prevent protease and HDAC activity ...
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bioRxiv - Genomics 2020Quote: ... 2 g of DUX4 expression constructs were transfected into RD cells with 6 L of the X-treme GENE 9 DNA transfection reagent (cat. XTG9-RO, Roche Diagnostics, Indianapolis, USA) diluted in 100 L of Opti-MEM I (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and 5 µg of a WEAU gp160 plasmid (pcDNA3.1-WEAU gp160) into exponentially dividing 293T/17 cells using FuGENE 6 (Roche Applied Science, Indianapolis, IN) or into 293F cells using 293fectin (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Dynamic monitoring of cell growth was determined every 12 hours during 6 days using the impedance-based xCELLigence system (Roche Applied Science, Germany). The cell index was derived from measured cell-electrode impedance that correlates with the number of viable cells.
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 1 mM EGTA, 10% glycerol, 1% Triton X-100, 1 mM DTT, 1 mM PMSF, 1× Roche protease inhibitor cocktail) and about 500 μL of 0.5-mm-diameter glass beads ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Biophysics 2020Quote: ... The 3′ end of the 1,882-nt DNA handle was labeled with dig-ddUTP using terminal transferase (Roche), and the 798-nt DNA handle of the transcript was functionalized with biotin on the 5′ end of the PCR primer ...
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bioRxiv - Cell Biology 2022Quote: ... The sonicated sample was layered over 3 ml of S3 solution (0.88 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) in a new Falcon tube and centrifuged at 3000 × g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... 5 µL of 20 mM siRNAs and 3 µL of X-tremeGENE HP DNA Transfection Reagent (Roche, #6366546001) were diluted in 200 µL Opti-MEM (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... purified GST-RomA was incubated with equal amounts of HIS6-LphD or HIS6-LphD Y392F in protein binding buffer (25 mM Tris pH 8.0, 140 mM NaCl, 3 mM KCl, 0.1% NP40 with protease inhibitors (ROCHE)) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The 2nd strand was synthesized using 2nd primer (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNNNNGTGGTATCAACGCAGAGTAC -3) with KAPA HiFi HS mix (KAPA Biosystems). The double stranded cDNAs were amplified using Illumina adapter-specific primers and LongAmp Taq DNA polymerase (NEB) ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Microbiology 2022Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 x g for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and single-cell suspensions of lung mononuclear cells were prepared by Liberase Blendzyme 3 (70 ug/ml, Roche) digestion containing DNaseI (30 µg/ml ...
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bioRxiv - Genetics 2022Quote: ... Precise knock-in of 3’ homologous arm was evaluated by PCR using KAPA2G Fast HotStart ReadyMix (KAPA Biosystems) with a primer set (5’-CCACTAGTTCTAGAGCGGC-3’ and 5’-GAACTGTTGGCTACTAACACTAATACTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were then prepared from 3 ng of CUT&RUN DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... The DNA probes were 3′ end-labelled using digoxigenin (DIG)-11-ddUTP and terminal transferase (Roche, Basel, Switzerland). Binding of TFRs to DNA was analyzed using the DIG Gel Shift Kit ...
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bioRxiv - Plant Biology 2024Quote: ... and the reducing agent 1,4-dithiothreitol (DTT, 10 mM; CAS#: 3483-12-3, Roche Diagnostics GmbH, Basel, Switzerland). The algae were incubated in ASW in the absence or presence of either one of the reagents for 30 min in the dark prior to the high light treatment.
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 3 min with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8 ...
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bioRxiv - Microbiology 2024Quote: ... and PcAEV5-3’ (nt 11,547-12,331) were prepared with specific primer pairs (Table S1) using PCR DIG Labeling Mix (Roche). Riboprobes were also prepared using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Immunology 2024Quote: ... Mouse lungs were excised and lightly homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...
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bioRxiv - Physiology 2024Quote: ... then diluted 10x in protein lysis buffer (3% SDS, 50 mM HEPES pH 8.5, 75 mM NaCl + Roche complete cocktail of protease inhibitors) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mM Tris pH 7.4, 0.5 mM EDTA, 5 mM KCl, 3 mM MgCl2, protease inhibitor, and phosphatase inhibitor cocktail from Roche). A 500 µl aliquot of the lysate was reserved for total liver proteome analysis ...
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bioRxiv - Cell Biology 2024Quote: ... the glutathione beads were washed 3 times with 1X MAPK Lysis Buffer with 1X protease inhibitor (Complete, Roche). Proteins bound were eluted using 1xSDS loading buffer and boiled at 70°C for 10 minutes before SDS-PAGE using a stain-free gel (1610183 ...
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bioRxiv - Biochemistry 2020Quote: ... 1% NP-40, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 1 mM sodium orthovanadate, 1 mM PMSF and Roche’s protease inhibitor tablet (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were dissected using the reference mask image from serial section 6 to collect regions of interest using medium or large AVENIO Millisect milling tips (Roche Sequencing Solutions, Pleasanton, CA), collected with Molecular Grade Mineral Oil (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4°) in lysis buffer (50 Tris-HCl, 150 mM NaCl, Roche complete EDTA-free protease inhibitor); centrifugation for 30 min at 4° and 21,000xg allowed isolation of the soluble fraction supernatant.
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bioRxiv - Plant Biology 2021Quote: ... After 4 weeks of culture on MSD medium supplemented with 50 mg/L hygromycin (Roche, Germany), the resistant callus lines were transferred onto RM plates to generate transgenic rice seedlings ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg/ml AEBSF [4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride] and protease inhibitors cocktail (Complete, Roche). Cells were cracked by multiple passages through a microfluidizer system using a pressure of 18’000 psi ...
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bioRxiv - Genomics 2021Quote: ... The resulting libraries were amplified with 4 PCR cycles using KAPA HiFi Uracil+ PCR enzyme (Roche). Libraries were quality controlled on a TapeStation 2200 HSD1000.
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bioRxiv - Neuroscience 2023Quote: ... PH 7.5)) and then incubated overnight at 4 °C in anti-DIG POD antibody (Roche, 11207733910) diluted 1:300 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were incubated overnight at 4°C in horseradish peroxidase-coupled anti-FITC antiserum (11426346910, Roche) diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were incubated overnight at 4°C in alkaline phosphatase-coupled anti-FITC antiserum (11426338910, Roche) diluted 1:5000 in blocking buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The membranes were probed overnight at 4°C with monoclonal anti-GFP primary antibodies (Roche, 11814460001) at 1:1,000 and then with anti-mouse peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2022Quote: ... Then the samples were incubated overnight at 4 °C with proliferation marker Ki67 (790–4286, Roche). After 3 washes with PBS ...
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bioRxiv - Immunology 2022Quote: ... were coated O/N at 4 °C with 50 μl of fibronectin (50 μg/ml; Roche) or VCAM-1-Fc (10 μg/ml R&D System) ...
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bioRxiv - Cell Biology 2024Quote: ... human skin was cut into thin pieces and incubated overnight at 4°C with dispase (Roche) at 2.4 U/ml ...
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bioRxiv - Microbiology 2024Quote: Cells (4 mg of lysates) lysed by RIPA lysis buffer (Pierce) containing protease inhibitor cocktail (Roche) were immunoprecipitated with pre-incubated mixture of 50 µL of protein A/G magnetic beads (Pierce ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.3% Tween-20 – pH7.5) for 4 min and in blocking solution (DIG Northern starter kit, Roche, #12039672910 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were centrifuged (10 min, 200 g, +4°C) and measured on a BioHT (Roche, Switzerland), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% P/S and 1 mg ml-1 collagenase A (Roche) for 90 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... + 1 mg ml-1 collagenase (Roche) + 100 U ml−1 hyaluronidase (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... GATA3 (L50-823; 1:1; Roche), E-cadherin (612130 ...