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Citations for Roche :
801 - 820 of 820 citations for Recombinant Mouse Epcam His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... tail clips or seminiferous tubules were digested and PCR was performed using KAPA HotStart Mouse Genotyping Kit (KAPA BIOSYSTEMS, Cat No. KK7352). The following primer pairs were used for determining genotype-Sirt1 genotyping ...
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bioRxiv - Genetics 2021Quote: ... extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D) coated with anti-GFP antibodies (Roche ref:11814460001). Proteins extracts were resolved on 4-15% polyacrylamide gels ...
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bioRxiv - Microbiology 2020Quote: ... The alkaline phosphatase conjugated anti-mouse secondary antibodies were used for hybridization and for detecting signals BCIP/NBT substrates (Roche Biochemical, Mannheim) was used.
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bioRxiv - Cell Biology 2021Quote: ... To determine the dimerization of sFeoFL::GFP and sFeoN:GFP a Western Blot was generated from a 4-12% gradient SDS and a Native polyacrylamide gel side-by-side using a mouse anti-GFP antibody (Roche Nr. 11814460001) at 1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: α-GDV1-GFP-DD and α-Pfs16 IFAs were performed with methanol-fixed cells using mouse mAbs α-GFP (1:200) (Roche, #11814460001) and α-Pfs16 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... ChIPs were performed by combining sonicated chromatin (500 ng DNA content) with either 1 μg mouse mAb α-GFP (Roche Diagnostics #11814460001) or 1 μg rabbit α-PfHP1 12 in incubation buffer [(5% Triton-X-100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Clones were screened for knockout of target genes by Western Blot and/or by allelic sequencing with custom primers (Integrated DNA Technologies; Table S2) after processing genomic DNA with the KAPA Mouse Genotyping Kit (KAPA Biosystems KK7352) and TOPO TA Cloning Kit (Thermo Fisher Scientific K457501).
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bioRxiv - Molecular Biology 2020Quote: ... The Dynabeads™ were washed once with 2 ml of PBS and coated with 15 μg of mouse monoclonal anti-GFP antibody (Roche Applied Science). The beads were finally washed three times with 2 ml of lysis buffer and added to the whole cell extract for a 2 h incubation with gentle rotation ...
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bioRxiv - Neuroscience 2019Quote: ... The PVDF membranes were treated and immersed into the rabbit anti-mouse PSD-95 monoclonal antibody (1:1000, Roche Group, Nutley, USA) to incubate at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... A single sample (one mouse) was assessed on each array plate on a Roche Light Cycler 480 II real time PCR thermal cycler (Roche, Basel, Switzerland). All data were analyzed with Qiagen’s Gene Globe online analysis application ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with primary antibodies diluted 1:100 (rabbit pAb α-γH2A.X from SigmaAldrich, H5912 and mouse mAb α-GFP from Roche, 11814460001) in 1% BSA (in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... In addition, the goat polyclonal antibody anti-Cta1p (Kragler et al., 1993) and the mouse monoclonal antibodies anti-GFP (Roche Diagnostics, cat. # 11814460001), anti-Pgk1p (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were blocked in 10% BSA and 0.05% Tween 20 in PBS for 30 minutes before application of one of the following antibodies: mouse anti-human p16INK4a (CINTEC-Roche, cat# 705-4793), rabbit anti-human alpha-smooth muscle actin or aSMA (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... The remaining right lungs and spleens of each A/J mouse treated with PBS or BNF 0.0005mg were macerated with protease inhibitor (complete, EDTA-free, Roche Life Science, IN, USA) and centrifuged ...
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bioRxiv - Cell Biology 2023Quote: RNA was obtained from epithelioids 1 week after medium change to mFAD or from mouse esophageal epithelium peeled and incubated with Dispase I (Roche catalog no. 04942086001) diluted at 1 mg/ml in PBS for 15 min ...
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bioRxiv - Pathology 2023Quote: FDB muscles were harvested bilaterally in sterile mouse ringer and maintained overnight in DMEM (supplier) supplemented with collagenase A (Roche, 0.2 mg/ml) and 1% Penicillin- Streptomycin in a CO2 incubator (37°C ...
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bioRxiv - Pathology 2020Quote: ... were performed at the National Mouse Metabolic Phenotyping Center (MMPC) at the University of Massachusetts Medical School using a Cobas Clinical Chemistry Analyzer (Roche Diagnostics, Indianapolis, IN, USA) while plasma non-esterified fatty acid (NEFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Classically activated macrophages were prepared by stimulating macrophages with 10 ng/mL lipopolysaccharide (LPS) (L5293, SigmalAldrich) and 10 ng/mL mouse interferon-γ (mIFN-γ, 11276905001, Roche, Basel, Switzerland). In loss-of-function experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...