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Citations for Roche :
801 - 819 of 819 citations for Recombinant Bovine FGF2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... were purified over a 1-mL column of anti-protein C affinity matrix according to the manufacturer’s protocol (Roche, cat. no. 11815024001). 1 mM CaCl2 was added to the supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... 500,000 HUVEC were washed with CUT&RUN wash buffer (20 mM HEPES pH 7.9, 150 mM NaCl, 500 nM spermidine, 1X Roche Protein Inhibitor Cocktail) at RT ...
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bioRxiv - Microbiology 2024Quote: ... respectively) was split in two samples with similar protein concentration of which one was mixed with Complete Protease Inhibitor (Roche, Mannheim, Germany) and the other one with ddH2O as a control ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Basel, Switzerland), and obtained by centrifugation at 13,000 g for 20 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... Whole cell extracts were prepared from cultured cells lysed at 4°C in RIPA protein buffer containing protease and phosphatase inhibitors (ref 11697498001, Roche, Bâle, Switzerland), and obtained by centrifugation at 13,000 × g for 20 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce, Rockford, ILL) with Complete Protease Inhibitor Cocktail Tablets (Roche, Indianapolis, IN). Extracts were cleared by centrifugation ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated human MIF was produced using D-biotinoyl- ε -aminocaproic acid-N-hydroxy-succinimide ester (Biotin-7-NHS) with the Biotin Protein Labeling Kit from Roche (Mannheim, Germany). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect electroporation-competent cells ...
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bioRxiv - Plant Biology 2021Quote: ... total protein was isolated from Agrobacterium infiltrated leaves using extraction buffer (50mM Tris-Cl, 1% β-Mercaptoethanol and protease inhibitor cocktail (Roche, Basel, Switzerland)) ...
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bioRxiv - Biochemistry 2020Quote: ... the expression medium containing the secreted fusion protein was supplemented with 1/4 tablet of protease inhibitor (complete EDTA-free protease inhibitor cocktail tablets, Roche Diagnostic GmbH, 45148300) and transferred to a 15 ml Falcon tube containing 200 μl of washed ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg of proteins from the microsomal membrane pellet (P13) were incubated with 30 μg/ml of proteinase K (Roche Diagnostics, Mannheim, Germany) for 30 min on ice ...
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bioRxiv - Developmental Biology 2022Quote: Testes from adult mice were cut into ~20 mg pieces and transferred into ~200 μl pre-cooled denaturing buffer from the Minute Total Protein Extraction Kit (SD-001, Invent Biotech, Plymouth, MN, USA) containing protease inhibitors (CO-RO, Roche, Indianapolis, IN, USA) and phosphatase inhibitors (PHOSS-RO ...
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bioRxiv - Cell Biology 2023Quote: ... were used to remove salts from purified protein and to exchange to freezing buffer (20mM HEPES, 100mM NaCl, 0.5mM EDTA, TCEP 0.5mM and Roche cOmplete™ Protease Inhibitor Cocktail), using the gravity protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The elute was neutralized with a PBS-diluted protein inhibitor cocktail (cOmplete, EDTA-free, protease inhibitor cocktail tablets: Roche Diagnostics GmbH, Mannheim, Germany) and used to infect TG1 cells ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue extracts were subjected to TCA precipitation as previously described53.Washed protein pellets were solubilized in 900 μl of urea buffer (8M urea, 150mM NaCl, 50mM Tris pH8, 1X Roche complete protease inhibitor). Protein quantitation was performed using Qubit fluorometry (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: Synaptosomes were prepared using the Procedure for Synaptic Protein Extraction from Neuronal Tissue” and Syn-PER Synaptic Protein Extraction Reagent (Thermo Fisher, #87793, containing a half tablet of cOmplete™ Protease Inhibitor Cocktail (Roche, #CO-RO) per 12.5 ml Syn-PER reagent ...
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bioRxiv - Plant Biology 2022Quote: ... mCIT tagged proteins were revealed by using respectively GFP monoclonal antibody (anti-GFP mouse monoclonal, Roche; at 1/1000 in 5 % milk over-night) as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unreacted compound was removed via centrifugation using a molecular weight cutoff filter or G-25 Sephadex Quick Spin Protein columns (Roche Applied Science, Indianapolis, IN).
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bioRxiv - Biochemistry 2023Quote: ... 100 ng of recombinant PfCDPK1 protein was taken in a kinase assay buffer (50 mM Tris, 50 mM MgCl2, 1× phosphatase inhibitor cocktail (Roche Life Science, Indianapolis, IN), 1 mM DTT ...