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Citations for Roche :
801 - 850 of 6368 citations for 7 Hydroxy 4 methoxy 1 indanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 1× protease inhibitor (Roche) and 10 U/mL SUPERase-In RNase Inhibitor) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× protease inhibitor (Roche)) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1× Protease Inhibitor (Roche) and 1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... with 1% BSA (Roche) and 0.2% skimmed-milk (Easis ...
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bioRxiv - Biochemistry 2024Quote: ... and 1× PhosSTOP (Roche). Sonication was performed with a Bioruptor (Diagenode ...
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bioRxiv - Immunology 2021Quote: ... 20 mM Tris pH 7.5, 1% Triton X-100 supplemented with 1× protease inhibitor and 1× phosphatase inhibitor; Roche, Basel, Switzerland). Lysates were cleared by centrifugation at 10,000 r.p.m ...
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bioRxiv - Neuroscience 2021Quote: ... or precleared for 1 hr using 30 μl dry volume of a 1:1 mixture of protein-A and protein-G agarose (Roche Diagnostics). Solubilized membranes (3 mg of total membrane protein ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Tween 20) for 20 min followed by 1 h incubation with alkaline phosphatase-anti-digoxigenin antibody (1:500, Roche, 1093274). Alkaline phosphatase activity was detected by incubating slides with Fast Red substrate (DAKO ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 pseudotyped virus was generated by co-transfection of the pCAGGS-S with the viral packaging plasmid psPAX2 and pLenti-EGFP/luciferase-expressing plasmid as a proportion of 1:1:1 into HEK293T cells using the X-tremeGENE HP DNA transfection reagent (Roche, 06366546001) according to the manufacturer′s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were subsequently incubated in 1 ml of blocking solution (1:1 (v/v) of Western Blocking Reagent (Roche, Cat#11921681001) and PBT ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, 1x Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Developmental Biology 2022Quote: ... 5x SSC, 0.05mg/ml Heparin, 0.25% Tween20, 1% SDS, 1 mg/ml Salmon Sperm DNA, 1 mg/ml Roche blocking buffer powder) containing oligo probes at a concentration of 1μg/120μl and hybridized for two days at 43°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated with the secondary antibodies 1:1000 in PBST supplemented with 1 % DMSO and 1 % blocking reagent (Roche, #1096176) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... If stained with phalloidin (1/500, Scientific Laboratory Supplies P1951) and DAPI (1/1000 in PBS from 1 mg/ml stocks, Roche 10236276001), or just DAPI ...
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bioRxiv - Plant Biology 2023Quote: ... 0.2 mM EDTA, 0.3 M NaCl, 1 M Urea, 1% IGEPAL CA-630, 1 mM PMSF, and Roche protease inhibitor cocktail) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail). The beads were eluted with 1xSDS sample buffer and subjected to SDS–PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% glycerol, 1 mM EDTA, 1.5 mM MgCl2, 300 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) for 30 min on ice to isolate the nuclear fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... pH7.9, 1 mM EDTA, 1.5 mM MgCl2, 150 mM NaCl, 0.5% NP 40, 1 mM dithiothreitol, 1 × Roche protease inhibitor cocktail) and eluted with 30 µl of 2 × SDS sample buffer and subjected to western blot (WB ...
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bioRxiv - Plant Biology 2022Quote: ... 1 X protease inhibitor cocktail and 1 X phosphatase inhibitor cocktail from Roche) ...
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bioRxiv - Immunology 2021Quote: ... and 10% glycerol) containing 1 mM PMSF and 1 × protease inhibitor cocktail (Roche). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EGTA and 1% Triton X-100 with protease/phosphatase inhibitor (Roche) mixture ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) with 1 mM PMSF and protease inhibitor cocktail tablets (Roche) by vigorous shaking in a fast prep cell breaker (Bio 101 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mM DTT and 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche). Fifty micrograms of total protein were loaded onto SDS-PAGE gels and separated by electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1 mM EDTA and 1×Complete EDTA free-tablet (Roche Diagnostics, Basel, Switzerland) in distilled water ...
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bioRxiv - Microbiology 2024Quote: ... Lysozyme 1 mg/ml and 1 tablet of EDTA-free protease inhibitor (ROCHE). Cells were broken passing twice through an Emulsiflex C5 (ATA scientific ...
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bioRxiv - Neuroscience 2023Quote: ... in a 1:1 ratio and added to white 384 well plates (Roche). Plates were run on a 45-cycle protocol using the LC 480 II system (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM EDTA and supplemented with 1× Complete protease inhibitor cocktail (Roche) by using a Teflon pestle (Schuett Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM DTT and 1 tablet of complete EDTA-free (Roche, Basel Switzerland)] ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM Na3VO4 and 1 mM NaF) supplemented with protease inhibitor cocktail (Roche) and incubated on ice for 30-60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 25mM NaCl, 1 mM EGTA, 1.5 mM MgCl2, 1 mM DTT, 1% Triton TX-100, 10% Glycerol, 1X Roche Complete protease inhibitors) during 1h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissues were minced into 1×1 mm fragments and enzymatically dissociated in HBSS containing 1 mg/ml collagenase A (#11088793001; Roche, Basel, Switzerland) and 1 μg/ml DNase I (#07900 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets were lysed in 1500 μl ice cold RIPA buffer (50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP40, 0.5% Na-deoxycholate, 0.1% SDS, 1 mM EDTA, Roche cOmplete and 1 mM PMSF). Cell lysates were clarified by centrifugation (13000 x g at 4°C for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 mM EDTA) supplemented with 1 mM PMSF and 1 tablet of cOmplete Protease inhibitor (Roche, cat. No. 11 873 580 001) per 50 ml using a MP Biomedical Fast-Prep-24 5G bead beater ...
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bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 200 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... volume of lysis buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 1 μg ml−1 benzonase, 1 mM PMSF and Roche protease inhibitor tablets). Cells were lysed by dounce homogenization and the NALCN complex was subsequently solubilized by addition of 2% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting pellet was washed four times with buffer II (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 1% triton X-100, 1 mM DTT, 0.1mM PMSF, 1% Roche protease inhibitor cocktail tablet) until the green color was completely removed ...
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bioRxiv - Microbiology 2020Quote: ... The cross-linking reaction was quenched by incubating the cells with 0.125 M glycine for 5 min with mild agitation at room temperature followed by centrifugation at 3,000 rpm for 5 min at 4°C with a subsequent PBS wash (containing 0.01X protease inhibitor cocktail or PIC; #11836170001, Roche Applied Science, Indianapolis, USA). Following the complete removal of PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-bromo-4-chloro-3-indolylphosphate (BCIP) and nitroblue tetrazolium chloride (NBT) according to the manufacturer’s protocol (Roche Applied Science, Indianapolis, IN, USA). The sections were then mounted ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by incubation (16 h at 4 °C) in the presence of 10 pM TBST containing complete TM protease inhibitor cocktail (Roche Pharma, Basel, Switzerland). The membranes were washed three times with TBS (150 mM NaCl ...
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bioRxiv - Developmental Biology 2020Quote: ... for 16-20h incubation at room temperature and colorimetric signals were detected using Nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphase (NBT-BCIP; Roche Applied Sciences 11383221001). RNAScope ISH was conducted for FGF15 and Ptch1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were washed and the secondary antibodies applied (dilutions listed below) with 4′-6-diamidino-2-phenylindole (DAPI: Cat # 10-236-276-001, Roche Diagnostics, Indianapolis, IN) at 1:1,000 for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The band corresponding to the crosslinked nanobody-antigen complex was then excised from the gel and subjected to in-gel digestion with trypsin (Roche, 1μg, 4 h) or chymotrypsin (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Antimouse secondary antibodies conjugated with alkaline phosphatase was used to detect color signal formed by BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) substrates (Roche Biochemical, Mannheim, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Cells at 70% confluence were harvested by trypsinization (after 3-4 hours treatment with 100 ng/mL colcemid [Roche #10295892001] for metaphase spreads), washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Oocytes were then incubated for 60 min at 4 °C with 100 μg/ml rat monoclonal anti-HA antibody (clone 3F10, Roche Pharmaceuticals, Basel, Switzerland) in 1% BSA/ND96 ...
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bioRxiv - Cancer Biology 2024Quote: ... Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment) using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, MA, USA). Poly-A mRNAs were purified from 100 ng of total RNA using poly-T-oligo-magnetic beads (Kapa Biosystems ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... 1% NP-40, 0.5% sodium deoxycholate, 1 mM EDTA pH 8 and 1 tablet of protease inhibitor cocktail Roche cOmplete (Roche, Basel, Switzerland) and 1 tablet of phosphatase inhibitor PhosSTOP (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the primary antibodies diluted in blocking solution (H3K9me3: ab8898, 1:500, HP1α: CST #2616, 1:200, HA: Roche 3F10, 1:250) for 1h at room temperature ...