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Citations for Roche :
801 - 850 of 2352 citations for 6 Methyl 8 2 deoxy b D ribofuranosyl isoxanthopterin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein translation was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, 11411446001, Roche Diagnostics) and cultures were incubated overnight at 18 °C ...
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bioRxiv - Plant Biology 2021Quote: ... collected and the enzymatic assay was performed using red-β-D-galactopyranoside (CPRG, Roche Applied Science) as substrate as described (Yeast Protocols Handbook ...
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bioRxiv - Immunology 2022Quote: ... CNS was minced with a razor blade and digested with collagenase D (2.5mg/ml, Roche Diagnostics) at 37°C for 20 min ...
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bioRxiv - Physiology 2022Quote: ... the spleen was additionally digested in HBSS medium containing 1 mg/ml Collagenase D (Roche, 11088882001) and 50 U/ml DNase I for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% n-dodecyl β-D-maltoside [DDM] and 1X cOmplete™ EDTA-free protease inhibitor (Roche)) and incubated at 4°C for 30 mins ...
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bioRxiv - Immunology 2022Quote: ... disrupted in 10 ml of RPMI (1%FBS) in the presence of Collagenase D (1X) (Roche) using a MACs dissociator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... inguinal adipose tissue was digested in a PBS buffer containing 10 mg/mL collagenase D (Roche), 2.4 U/mL dispase II ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissue fragments were digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... skeletal muscle tissue was enzymatically digested with 1.5 U mL−1 collagenase D (Roche, Ref. 11088882001), 2.4 U mL−1 dispase II (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: The vaccine-draining popliteal LN was incubated with 3ml of filtered 1mg/ml collagenase D (Roche) for 30 minutes at 37 C’ ...
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bioRxiv - Immunology 2022Quote: Tumor draining lymph nodes (tdLNs) were minced and digested with Collagenase D (1 mg/mL, Roche) and DNAse I (50 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... It was then minced and digested in HBSS containing 10 mg ml-1 Collagenase D (Roche), 3 U ml-1 Dispase II (Roche) ...
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Inhaled CpG increases survival and synergizes with checkpoint inhibition in lymphangioleiomyomatosisbioRxiv - Immunology 2023Quote: ... Left lung lobes were dissociated by enzymatic digestion with 1 mg/mL collagenase D (Roche, 11088882001), and 1 mg/mL collagenase IV (Worthington Biochemical ...
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bioRxiv - Immunology 2023Quote: ... tumors were cut into small pieces and enzymatically digested using 1mg/ml Collagenase D (#11088882001, Roche), and 0.1mg/ml DNase I (#101104159001 ...
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bioRxiv - Immunology 2023Quote: ... kidneys were then incubated for 15 minutes in DMEM containing 1 mg/ml Collagenase D (Roche), 100U/ml DNAse I (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... fresh sinonasal tissue samples underwent enzymatic digestion with a mixture of 25mg/mL collagenase D (Roche Diagnostics GmbH ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 1% n-dodecyl β-D-maltoside (DDM) and protease inhibitor cocktail (4693116001, Roche, USA). Homogenization was achieved by gently mixing on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in digestion solution (3 mg/mL collagenase D [Roche] in DMEM/F-12 [Gibco]) for 45 min at 37 °C with intermittent vortex mixing to dislodge soft tissues ...
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bioRxiv - Molecular Biology 2020Quote: Frozen extracted mitochondria were lysed by sonication in 300 μl of lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1X protease inhibitors (Complete mini EDTA-free mixture [Roche Applied Science] ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed in TNT buffer (1% Triton X-100, 150 mM NaCl, 50 mM Tris HCl, pH 8) with protease inhibitors (Roche). 5 uL of whole cell lysate (approximately 40 μg ...
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bioRxiv - Plant Biology 2020Quote: ... Cells were then harvested and resuspended in 8 mL of Native Purification Buffer (50 mM NaH2PO4, 500 mM NaCl) supplemented with 8 mg lysozyme and a protease inhibitor tablet (Roche). The suspension was then incubated on ice for thirty minutes ...
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bioRxiv - Cell Biology 2019Quote: 10 heads from animals at 8 DAE were resuspended in 100 μl of Hepes/NaOH 50mM with proteases and phosphatases inhibitors (Roche), samples were disrupted using a mechanical tip following two rounds of sonication of 10 sec each ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, 1 mM TCEP, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested 48h after transfection and lysed in lysis buffer (50mM Tris-Cl pH 8, 150mM NaCl, 1% Triton, 1x Roche Complete Mini Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2019Quote: ... The cells were washed twice with PBS and lysed with 6 ml of lysis buffer (8 M urea, 150 mM NaCl, 100 mM ammonium bicarbonate, pH 8; added per 10 ml of buffer: 1 tablet of Roche mini-complete protease inhibitor EDTA free and 1 tablet of Roche PhosSTOP tablet ...
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bioRxiv - Cell Biology 2021Quote: ... air-dried and resuspended in 50 µl of 8 M urea in 50 mM triethylammoniumbicarbonate including protease inhibitor cocktail (Roche). Afterwards proteins were reduced with 5 mM dithiothreitol for 1h at 25°C and alkylated with 40 mM chloroacetamide for 30 min in the dark ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and resuspended in Wash Buffer (150mM NaCl, 100mM Tris-HCl, pH 8) and a Complete protease inhibitor tablet was added (Roche). Cells were lysed by sonication (25% amplitude 1 sec on ...
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bioRxiv - Biochemistry 2021Quote: ... Then qPCR was performed in triplicate using 4 μl sample in 8-9 μl reaction with FastStart Universal SYBR Green Master Mix (Roche) and primers flanking each MseI cassette (Table S3).
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bioRxiv - Cell Biology 2021Quote: U2OS cells were seeded into 8-well Imaging Chamber CG (Zell-Kontakt) and transfected 48-72 h prior to imaging using XtremeGene HP (Roche) according to the manufacture’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 300 μL of Nuclei Lysis Buffer (Tris-HCl pH 8 50 mM, EDTA 10 mM, SDS 0.5% and protease inhibitor (Roche-04693132001). Nuclei were then sonicated (Diagenode bioruptor plus sonicatore ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by centrifugation at 7000g at 4°C for 10 minutes and resuspended in 25 mL of cold TBS buffer (50 mM Tris-HCl, 500 mM NaCl, pH 8) with 5% glycerol and EDTA-free protease inhibitors cocktail (Roche). Cells were lysed by sonication and purified using Glutathione-Sepharose 4B (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... cells were recovered and lysed in 500 µl hypotonic lysis buffer (10 mM Tris-HCl pH 8, 10 mM KCl, 1× complete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2022Quote: ... collected and lysed with NP-40 extract buffer (50 mM Tris, pH 8, 150 mM NaCl, 1 % NP-40) containing protease inhibitors (#11873580001 Roche) and 30 µg of lysate was migrated in 10 % SDS-PAGE gels (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... the culture medium was replaced with serum-free medium 8 h prior to addition of IFN-γ (1000 IU; Roche). In experiments with Tofacitinib ...
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bioRxiv - Plant Biology 2021Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, CA) to reduce the likeliness of multiple identical reads due to preferential amplification ...
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bioRxiv - Plant Biology 2021Quote: ... For IPs 0.3 g of flower buds were ground in 1.5 ml of ice-cold lysis buffer (50 mM Tris-HCl pH 8, 50 mM NaCl, 1 % Triton X-100, supplemented with Roche cOmplete ...
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bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
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bioRxiv - Cancer Biology 2019Quote: ... 2 μl of the purified cDNA was mixed with 8 μl of a qPCR mixture [1.25x KAPA HiFi HotStart Readymix (KAPA Biosystems), 0.625 μM PCR lnPE1 primer ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were washed three times with PBS and harvested directly in 8 M Urea lysis buffer supplemented with phosphatase inhibitor (PhosSTOP, Roche) and protease inhibitor (cOmplete mini EDTA-free ...
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bioRxiv - Genetics 2021Quote: ... Probes were generated using primers listed in Supplementary Table 1 from the G135P65476A4 BAC as described in [8] and were labeled using the DIG-Nick Translation Mix (Roche) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Harvested cells were washed once with PBS and resuspended in His-Trap binding buffer (50 mM Tris, pH 8; 150 mM NaCl; 20 mM imidazole; protease inhibitor cocktail (Roche) and 50 μM NBQX to displace glutamate and promote dimerization) ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
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bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mM EDTA at pH 8, 10% glycerol, 1 mM DTT, 0.5 mM PMSF, 0.1 mM sodium orthovanadate, and 1X Roche protease inhibitors). Collected nuclear pellets were lysed in in 1× RIPA buffer (10 mM Tris-Cl at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: The HEK293 cell pellet was resuspended in 25 mL IEX buffer A (50 mM Tris, pH 8) with protease inhibitors (cOmplete, EDTA-free cocktail, Roche) and sonicated 20 s on ...
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bioRxiv - Cell Biology 2019Quote: ... The qPCR reaction was carried out in 8 μl with a primer concentration of 1 μM and SYBR Green Master mix (Roche) in a Roche LightCycler 480 system ...
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bioRxiv - Microbiology 2020Quote: ... coli cells were resuspended in 200 mL of cold Lysis buffer (50 mM HEPES pH 8, 300 mM NaCl, 1 mM EDTA, 5 % glycerol, 4 tablets of protease inhibitors, Roche). The cells were lysed and the membrane fraction was collected as described in the YukC purification protocol below ...