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Citations for Roche :
801 - 850 of 2903 citations for 6 Chloro 9 2 C methyl β D riboFuranosyl 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured 6 days post transfection using WST-1 reagent (Roche). Cells were incubated with 10 μl of WST-1 per 100 μl of media for 1 hour and absorbance was read at 450 and 630 nm ...
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bioRxiv - Plant Biology 2021Quote: ... with gene-specific primers (Supplementary Table 6) on a LightCycler 480 system (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Tumors were transferred into 6-well plates containing 0.25mg/mL LiberaseTL (Roche, 5401020001) and 0.33mg/mL DNase (Sigma-Aldrich DN25-10MG ...
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bioRxiv - Neuroscience 2022Quote: Recombinant lentiviruses were produced by transfecting HEK293T cells using FuGENE®6 (Roche) with plasmids encoding viral enzymes and envelope proteins essential for packing of viral particles (pRSV-EV ...
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bioRxiv - Genomics 2023Quote: ... 6 μL of Ligation-1 mix (3.75 U T4 DNA ligase (Roche, 10799009001), 33.3 mM DTT (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... with 6-8 cycles of PCR amplification using KAPA-HiFi (Kapa Biosystems, KK2602). DNA library was sequenced using an Illumina NextSeq 500 75-cycle high output kit.
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Microbiology 2023Quote: ... Pools of 6 ganglia were homogenized in 1 ml TriPure isolation reagent (Roche) using lysing matrix D on a FastPrep24 instrument (3 cycles of 40 seconds at 6 m/s) ...
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bioRxiv - Cancer Biology 2023Quote: ... and recombinant mouse interleukin-2 (mIL-2, 100 U/ml; Roche, Basel, Switzerland), and subjected to flow cytometry as described elsewhere ...
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bioRxiv - Neuroscience 2023Quote: ... 150 μL of RIPA double-detergent buffer (2% deoxycholate, 2% NP-40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with protease inhibitor cocktail (Roche, cat# A32953) was added to the tissue homogenate followed by incubation on a shaker for 1 h at 4ºC ...
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bioRxiv - Microbiology 2022Quote: ... 45 cycles of 95°C for 3 s and 60°C for 30 s using the LightCycler 96 system (Roche, Basel, Switzerland) or the StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... pursued by 95°C for 3 minutes and then 45 cycles at 95°C for 15 seconds and 58°C for 30 seconds using a LightCycler 480 Real-Time PCR system (Roche, Rotkreuz, Switzerland). The primers and the probes were designed against the E gene (20).
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bioRxiv - Immunology 2020Quote: ... cultured in vitro at 30 °C and at 37 °C in EMJH medium was also extracted using a High Pure RNA Isolation kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were incubated overnight at 4°C in Odyssey blocking buffer containing 0.1% Tween-20 at 4°C and 1/1000 dilution of anti-c-myc mouse monoclonal antibody (Catalogue number 11 667 149 007, Roche, Bâle, Switzerland) or anti-β-tubulin mouse antibody (Catalogue number T9026 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted at 500 x g for 5 min at 4°C and resuspended in 10 ml 4 °C RSB supplemented with protease inhibitor (cOmpletetm, EDTA-free protease inhibitor cocktail, Roche, Cat#: 11873580001) and incubated on ice for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... in lysis buffer (50 mM Tris–HCl pH 7.5, 300 mM NaCl, 10 mM imidazole, 5 mM β-mercaptoethanol, 0.5% Triton X-100, Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 mM KCl, 1 mM MgCl2, 0.5 mM β-mercaptoethanol, 0.1 mM GTP, 3 U/ml benzonase, 1X Roche Complete EDTA-free protease inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... β-galactosidase activity was detected in prepared embryos through use of either the X-gal or Salmon-gal (Roche) substrates prepared as follows ...
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bioRxiv - Biochemistry 2021Quote: ... 20 mM imidazole and 5 mM β-mercaptoethanol (BME)) and supplemented with DNase I and protease inhibitor cocktail (Roche). Cells were disrupted by Emulsiflex C3 (Avestin ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin, 1mM sodium pyruvate, 0.1% β-mercaptoethanol, 25mM HEPES, 150μg/ml DNase I [Sigma-Aldrich], 0.5mg/ml Liberase [Roche] and then slow spun at 50xg for 5 mins (11) ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 1% SDS, 0.1 mM dithiothreitol, 20 μM PMSF, 0.5% β-mercaptoethanol, 1X proteinase inhibitor from Roche) and centrifuged for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Ni-NTA agarose was rotated for 30 min at 4°C with 10 mL of elution buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 1.4 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was thawed in two volumes of lysis buffer (3M NaCl, 100mM Tris pH7,5, 10% Glycerol, 0.5mM EDTA, 5 mM β-mercaptoethanol, protease inhibitors (Roche)) and sonicated on ice (10 pulses of 10 seconds) ...
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bioRxiv - Genomics 2020Quote: ... Paraffinized at 72°C with EZ solution (Roche Ventana). Antigen for SOX17 was retrieved via CC1 protocol with prediluted Tris solution ...
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bioRxiv - Cell Biology 2019Quote: ... The anti-c-Myc antibody was purchased from Roche. Restriction enzymes and Gibson assemble master mix was purchased from New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4 °C overnight and protein G-Agarose (Roche) at 4 °C for 3 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 sec at 97°C in a LightCycler96 (Roche).
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bioRxiv - Cancer Biology 2024Quote: ... and Proteinase K (Roche; 1 h at 55 °C) and DNA recovered using PCR purification kit (Qiagen).
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bioRxiv - Developmental Biology 2020Quote: ... iWAT depots were digested in PBS containing collagenase D (Roche, 1.5 U/ml) and dispase II (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lymph nodes were then digested with 0.4 Wunsch Units/ml Collagenase D (Roche) and 250 µg/ml DNAse I (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Lymph nodes were incubated in 400 U/ml collagenase D (Roche Applied Science) for 30 min before filtration through a 70 μm filter ...
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bioRxiv - Developmental Biology 2022Quote: ... Bones and marrow were placed in 3mg/mL of Collagenase D (Roche 11088866001) for 30 minutes in hypoxia ...
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bioRxiv - Neuroscience 2020Quote: ... and probes were labeled with digoxigenin-11-d-UTP (Roche Diagnostics, Indianapolis, IN). Tissue preparation ...
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bioRxiv - Immunology 2020Quote: ... Fat depots were digested in PBS containing collagenase D (Roche, 1.5 U/ml) and dispase II (Sigma ...
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bioRxiv - Immunology 2019Quote: ... chopped finely and digested in 0.5ml pre-warmed collagenase D (1mg/ml; Roche) in RPMI 1640 media supplemented with 2% FCS for 15 minutes in a shaking incubator at 37°C ...
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bioRxiv - Genetics 2020Quote: ... and incubated with 400 U collagenase D (#11-088-858, Roche applied science) for 30 min at 37°C ...
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bioRxiv - Immunology 2019Quote: Duodenal samples were incubated in R10 media with 1mg/ml Collagenase D (Roche) and 100μg/ml DNase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and after mincing incubated with 2.5 mg/mL Collagenase D and DNAse (Roche) for 20 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Tissues were then transferred to a solution of 0.5mg/ml Collagenase D (Roche) and 15μg/ml DNase I (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Small tissue pieces were digested with 0.5 mg/ml collagenase D (Roche 11088882001) and 5 U/ml DNAse 1 (Sigma-Aldrich 10104159001 ...
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bioRxiv - Immunology 2023Quote: ... and Collagenase D (125 μl of 40 mg/mL solution; Roche; Basel, Switzerland) were added to tumor samples prior to incubation on a shaker at 37° C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Tissue was digested in RPMI 1640 containing 1.25 mg/ml collagenase D (Roche), 0.2 mg/ml of Dispase II (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Spleens were disrupted against a nylon screen and treated with Collagenase D (Roche) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The minced muscles were then incubated in 0.1% collagenase D (Roche, Basel, Switzerland) and 0.1% trypsin in Ham’s F12 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (2 mM activated Na3VO4, 2 mM NaF and 1x PhosSTOP (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... plates were fixed with 2% paraformaldehyde/ 2% sucrose and stained with DAPI (#10236276001, Roche). Plates were photographed with the IN Cell Analyzer 2200 high content analyzer (GE Healthcare) ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20] and maintained for 2 hours in 2% blocking reagent (11096176001, Roche) in TNT ...
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bioRxiv - Immunology 2024Quote: ... 2 mM L-Glutamine (R10) (Thermo 25030081) + 10 IU/mL IL-2 (Roche 11011456001) after CD3/CD28 stimulation ...
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bioRxiv - Genomics 2019Quote: ... Index tagged samples were amplified (6 cycles of PCR, KAPA HiFi kit, KAPA Biosystems), quantified (Accuclear dsDNA Quantitation Solution ...