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Citations for Roche :
801 - 850 of 8327 citations for 5 Methoxy 1 Triisopropylsilyl 1H Pyrrolo 2 3 B Pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM ATP (99% Roche) and 20 mM MgCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 5% Normal Goat Serum) for 3 h at room temperature and then incubated overnight with alkaline phosphatase-conjugated anti-DIG Fab fragments (Roche, 1: 2,000) at 4°C on a shaking platform ...
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bioRxiv - Neuroscience 2020Quote: ... 1996) on 16 µm cryosections at post-natal day 3 (P3) using anti-digoxigenin antibody (Sheep polyclonal, 1:1000, Roche, 11093274910, RRID:AB_514497). Two days exposure to alkaline phosphatase (AP ...
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bioRxiv - Physiology 2021Quote: ... a 6.7 kb DNA fragment starting 1 kb upstream of murine Gnrhr exon 3 was amplified by PCR using the Expand Long Template PCR System (Roche, Basel, Switzerland) from 129SvEv genomic DNA using primers incorporating 5’ XmaI and 3’ NotI restriction enzyme sites (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were lysed in 50 µl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 1× Roche Complete protease inhibitors cocktail) by pipetting up and down ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed with the same cold nuclei extraction buffer (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 3% triton X-100, 1% Roche protease inhibitor cocktail tablet) and then resuspended in nuclei purification buffer (20 mM MOPS pH 7.0 ...
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bioRxiv - Plant Biology 2024Quote: ... were finely chopped into pieces in cold nuclei extraction buffer (10 mM Tris–HCl pH 8.0, 10 mM MgCl2, 0.25 M sucrose, 3% triton X-100, 1% Roche protease inhibitor cocktail tablet). The resulting mixture was filtered through a 40 μm cell sieve into a collection tube and centrifuged at 1,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Plant Biology 2020Quote: ... 0.5 mM PMSF) supplemented with 5 mg L−1 DNAse I and 0.5 g/L lysozyme (Roche, Mannheim, Germany). Cells were disrupted by sonication (3×2 min ...
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bioRxiv - Plant Biology 2021Quote: ... 150 mM NaCl, 10% glycerol, 0.2% NP-40, 1 mM EDTA, 5 mM DTT, and EDTA-free protease inhibitor [Roche]). Anti-GFP agarose resin (Chromotek ...
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bioRxiv - Cancer Biology 2020Quote: ... 150 mM NaCl, 50 mM Tris pH 8.5, 40 mM chloroacetamide, 5 mM TCEP; freshly added: 1 tablet Phosphostop [Roche, 4906845001] and 1 tablet cOmplete Protease inhibitor cocktail [Roche ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5, 400 mM NaCl, 5% glycerol, 20 mM imidazole, 1 Mm DTT, Complete EDTA-free protease inhibitor [Roche]) and the cleared lysate was incubated with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then blocked for an hour in 5% milk powder/PBS solution and probed with 1:2,500 rat anti-HA mAb 3F10 antibody (Roche) in 5% milk powder/PBS solution overnight ...
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bioRxiv - Microbiology 2020Quote: ... 300 mM NaCl, 30 mM imidazole, 1 μM DNases, 0.75% CHAPS, 5[mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]), and lysed with high pressure cell homogenizer (Union-Biotech ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% milk and probed overnight at 4°C with 1:2,500 rat anti-HA mAb 3F10 (Roche). They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... diluted 10 times and 1 μl was used in 5-μl PCR reaction on a LightCycler 480 (Roche Diagnostics) with Luna Master Mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting organoids were further digested with warm 0.25% trypsin for 1 min with constant pipetting followed by treatment with 5 mg/mL Dispase (Roche) for 5 min in at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... bacterial pellets were resuspended using a high salt buffer (25 mM Tris pH7.5, 1 M NaCl, 10 mM imidazole, 5% glycerol) supplemented with protease inhibitor tablets (Roche), 0.5 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested by centrifugation and resuspended in lysis buffer (50 mM HEPES pH7.5, 2M NaCl, 5 mM MgCl2, 1 tablet of Complete Inhibitor cocktail EDTA Free (Roche) per 50◻ml ...
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bioRxiv - Genomics 2021Quote: ... containing 25 Abs260 ribosome units (1 A260 = 12 μg/μL) [5] were digested with 60 U of MNase (Roche) and 60 U of SUPERase·In (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-stimulated with 5 μg/mL CTH522 or 1 μg/mL phytohemagglutinin (PHA; Roche, cat.: 11249738001) as positive control ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 8 M urea buffer (8 M urea, 1 M Tris pH 7.4, 5 M NaCl) containing protease and phosphatase inhibitors (Roche) followed by sonication at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 1x PLB (10x PLB: 1 M KCl, 50 mM MgCl2, 100 mM HEPES-NaOH pH 7.5, 5% NP-40, Roche protease and phosphatase inhibitors (1 tab each per 10 mL) ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were then ground to a fine powder in a mortar and pestle over liquid nitrogen and resuspended in freshly prepared extraction buffer (2mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% v/v NP40, 5% v/v glycerol, 1mM PMSF, 1ml Roche Protease Inhibitor cocktail per 30g input tissue ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Cell Biology 2024Quote: Cells or tissues were washed with PBS and lysed in an appropriate amount of protein lysis buffer (TritonX100 -1%, Tris 7.4 -50mM, EDTA -5mM, NaCl -150mM, and glycerol -5%) containing Protease Inhibitor Cocktail (Roche). We used Phosphatase Inhibitor Cocktail (Millipore ...
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bioRxiv - Genomics 2022Quote: ... 25 mM potassium chloride, 5 mM magnesium chloride, 10 mM Tris-HCl pH 8.0, 1 μM DTT, 1x Roche Protease Inhibitor Cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... ground in a fine powder with liquid nitrogen and resuspended with 1 ml of Guanidine hydrochloride extraction buffer (6M GuaHCl, HEPES 1 M, EDTA: 5 Mm, adjusted to pH 7.5) supplemented with protease inhibitor cocktail (Roche). Homogenates were filtered through a 100 µm cotton mesh ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biophysics 2022Quote: ... 5 EDTA and 1% Triton X-100) supplemented with cOmplete™ Mini-EDTA free protease inhibitor cocktail (Roche, Germany). Lysates were incubated 5 min on ice and centrifuged 10 min - 14000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed 1x with PBS and lysed with lysis buffer (25 mM Tris-HCl at pH 7.5, 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001] ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...