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Citations for Roche :
8401 - 8450 of 10000+ citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 0,1% sodium deoxycholate and 1% NP-40 with Complete EDTA-free protease inhibitors (Roche) and sonicated 10 s on ice ...
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bioRxiv - Plant Biology 2022Quote: ... and 1 μl of water were processed in a LightCycler® 480 instrument (Roche) using the SybrGreen master mix (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... and staining was performed in 1x TNM supplied with 1:50 NBT/BCIP (Roche). The reaction was finally quenched with three baths of 5min in 1x TE buffer.
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bioRxiv - Cancer Biology 2023Quote: ... 1% IGEPAL CA-630) supplemented with complete protease and PhosSTOP phosphatase inhibitor cocktails (Roche). Mouse tissues were mechanically homogenized by manual grinding in liquid nitrogen before lysis in RIPA buffer ...
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bioRxiv - Plant Biology 2023Quote: ... supplemented with 1 μM phenylmethylsulfonyl fluoride and a complete protease inhibitor cocktail (Roche Diagnostic). The digestion process was carried out at 37°C for 1.5 hours with continuous shaking at 50 to 100 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were incubated with anti-DIG-AP Fab fragments antibody (Roche, 11093274910, 1:2000) for overnight ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Cancer Biology 2023Quote: ... 4.5 mg of psPAX2 and 1 mg of pVSVG with X-tremeGENE HP (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mM EDTA) supplemented with 1 mM PMSF and protease inhibitor cocktail (Roche #11836170001). To ensure complete lysis ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM β-mercaptoethanol) and complete EDTA-free protease inhibitor cocktail (Roche; Mannheim, Germany). Cells were lysed with 2 minutes of sonication at 50% power using a Branson Sonifier ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% NP-40) containing protease inhibitor cocktail (cOmplete, EDTA-free Protease Inhibitor Cocktail – Roche). Supernatants were collected after centrifugation at 12000 RPM ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also treated with 100 µM of T20 HIV-1 fusion inhibitor (Roche) or 3 µg/ml EFZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Flow cells were incubated with 1 μl of α-digoxigenin antibody (Roche, 150 U) diluted in 30 μl TB buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM 2-Mercaptoethanol and 6 cOmplete EDTA-free protease inhibitor Cocktail tablets (Roche), pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in alkaline phosphatase-labeled anti-DIG antibody (Roche; 1:600) in TNB for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM tris(2-carboxyethyl)phosphine [TCEP]) supplemented with EDTA-free protease inhibitors (Roche). The cells were lysed via sonication and lysate was clarified by centrifugation (17,000 rpm for 1 hour at 4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... 1% penicillin-streptomycin) and treated with 2 units/mL of DNase I (Roche Diagnostics) for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... after which they were exposed to WST-1 according to the manufacturer’s protocol (Roche). The Formazan absorbance of each sample was measured at a 450 nm wavelength using a Synergy 4 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... and protease inhibitor (1 pill per 10 ml; cOmplete Mini EDTA-free, Roche Diagnostics). The lysates were sonicated and then centrifuged at 14,000 x g for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 h in 1:1500 solution of anti-digoxigenin antibody (Roche, cat no. 11333089001):blocking solution ...
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bioRxiv - Genomics 2024Quote: ... Sections were blocked an incubated in anti-DIG-AP Fab fragments (1:600; Roche) for two hours and incubated in BCIP/NBT chromogen (PerkinElmer ...
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bioRxiv - Plant Biology 2024Quote: ... followed by 1-3 days in an NBT/BCIP (Roche, Cat No./ID: 11681451001) solution ...
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bioRxiv - Cancer Biology 2024Quote: ... at 1:100 for 8 minutes and KI-67 (Roche Tissue Diagnostic, ref. 05278384001) was applied for 20 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Viability was assessed 48 hours later using WST-1 Cell Proliferation Reagent (Roche, 11644807001) as described previously.23
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bioRxiv - Cell Biology 2024Quote: ... 0.1% sodium deoxycholate) supplemented with 1 mM PMSF and 1x protease inhibitor cocktail (Roche). The cells were broken using Zirconia/Silica beads (BioSpec ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 nM ADP-HPD (a PARG inhibitor; sigma) and 1× protease inhibitor cocktail (Roche)] and incubated for 30 min on ice with gentile vortexing and then centrifuge at maximum speed for 15 minutes at 4°C in microcentrifuge to remove the cell debris ...
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bioRxiv - Developmental Biology 2024Quote: ... After a 1-h pre-incubation in MABT buffer containing 10% blocking reagent (Roche) and 10% heat-inactivated lamb serum ...
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bioRxiv - Cell Biology 2024Quote: ... The hybridization result was detected with anti-DIG-AP antibody (1:2000, Roche, 11093274910) and NBT/BCIP (1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... first using MASC PCR (Supplementary Data 1) in KAPA2G Fast Multiplex MasterMix (Roche, USA) and then by validating its sequence using Illumina whole genome sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and sonicated in 1% PBS TritonX-100 (PBSTx) with 1x protease inhibitor (Roche, 04693132001). Debris was then pelleted ...
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bioRxiv - Molecular Biology 2024Quote: ... by incubating 1 μg of linearized plasmids with 1x transcription buffer (Roche Applied Science), 10 mM DTT ...
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bioRxiv - Cancer Biology 2021Quote: ... size and concentration of cDNA libraries were checked using the Standard Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Hiseq 2500 sequencer as single-end 125 base reads ...
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bioRxiv - Cell Biology 2019Quote: ... with ATP standard solutions used at concentrations between 10−8 and 10−5 M (luciferin/luciferase ATP bioluminescence assay kit CLSII, Roche, Basel, Switzerland). Data are expressed as nmol ATP produced/min/106 cells.
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... 4 µg of total RNA was used to generate libraries using of stranded poly(A) mRNA-Seq library with the KAPA stranded mRNA Library prep kit (KAPA Biosystems, KK8421). Libraries concentration was quantified with Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Microbiology 2019Quote: ... separated RNA was transferred to positively charged nylon membranes using overnight capillary transfer in 20X saline-sodium citrate (SSC) buffer as described in the DIG Northern Starter Kit manual (Roche, Cat. 12039672910).
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bioRxiv - Developmental Biology 2021Quote: ... One microgram of total RNA was reverse transcribed to cDNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics, Tokyo Japan) using an anchored-oligo(dT)18 primer and random hexamer primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Digoxigenin (DIG)- and biotin-labelled antisense probes of target IRs were transcribed from linearized pCS2+ vectors with the coding region of the corresponding receptors using the T7 RNA transcription kit (Roche, Mannheim, Germany). Labelled probes were subsequently fragmented to an average length of about 800 bp by incubation in sodium carbonate buffer (80 mM NaHCO3 ...
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bioRxiv - Developmental Biology 2021Quote: ... The digoxigenin (DIG)-labeled sense or antisense RNA probe for full-length Emi2 was synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). No signal was detected with sense probes ...
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bioRxiv - Developmental Biology 2021Quote: ... The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals). Two μg of probes was incubated with purified Flag-tagged proteins for 15 min ...
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bioRxiv - Physiology 2022Quote: ... was performed on the cDNA using the Sybr green method of quantification on a Roche Lightcycler 480 using a SYBR FAST quantitative PCR kit (Kapa Biosystems; KK4611). Gene expression was analyzed for each sample in triplicate using the forward and reverse primers indicated on Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems, catalog #KK4605). Primers (5’3’ ...
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bioRxiv - Genomics 2020Quote: ... a total of 100 ng DNA from each bacterial strain was used according to KAPA Hyper Prep kit (KAPA Biosystems, MA, USA) at the Georgia Genomics and Bioinformatics Core (GGBC) ...
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bioRxiv - Genomics 2020Quote: Each of the 10 plate libraries was quality assessed on an Agilent Bioanalyzer 2100 High Sensitivity chip and quantified by qPCR using the Illumina Kapa Library Quantification Kit (Roche, Cat. #KK4824). The libraries were then normalized to a concentration of 4 nM and pooled for sequencing ...
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bioRxiv - Genomics 2020Quote: ... Hybridization of the digoxegenin (DIG)-labelled 31bp oligonucleotide probe and detection was carried out following the DIG High-Prime Labelling and Detection Starter Kit (Roche SKU 11745832910).
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected 96 hours post-infection (hpi) and RNA was extracted with the High Pure RNA Isolation kit (Roche, cat#11828665001), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... was used to construct DNA libraries for sequencing on the Illumina platform using the KAPA Library Preparation Kit (Kapa Biosystems, Woburn, MA). DNA was fragmented with the Covaris E210 or E220 to ∼200 bp ...
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bioRxiv - Microbiology 2019Quote: The pooled library was prepared with 10 μl of barcoded PCR bead purified product from each individual sample and measured with KAPA qPCR library quantification kit (KAPA Biosystems, USA), before being run on an Illumina Mi-Seq Sequencer using a 600-cycle pair end reagent kit (Mi-Seq Reagent Kits v2 ...
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bioRxiv - Microbiology 2019Quote: ... The DNA from the oral samples and from the skin samples from the back were isolated using the MagnaPure LC DNA Isolation Kit III (Bacteria, Fungi; Roche, Mannheim, Germany) as described by Santigli et al ...
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bioRxiv - Developmental Biology 2019Quote: ... Sample preparation protocol for the RNASeq libraries was following the manufacturer’s recommendations of KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche-Kapa Biosystems). The libraries were sequenced on HiSeq2500 (Illumina ...